Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,065,027	G→T	A145D (GCT→GAT)	erfK ←	conserved hypothetical protein with NAD(P)‑binding Rossmann‑fold domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,065,027	0	G	T	100.0%	91.2 / NA	29	A145D (GCT→GAT)	erfK	conserved hypothetical protein with NAD(P)‑binding Rossmann‑fold domain
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (22/7);  total (22/7)

CCCCTCGTTTCGCATATTCGCGCCGAGTGTTCGGCGTTGGCGTCCAGGTTGGAGCTTCTTGTTTACGTTCAACGGTAGTCACCCAGTTACGCGGGGTTTCTCGCCCAGCCTGG  >  W3110S.gb/2064974‑2065086                                                      |                                                            ccccTTGTTTCGCATATTCGCGCCGAGTGTTCGGCGTTGGCGTCCAGGTTGGATCTTCTTGTTTa                                                  >  1:875158/1‑65 (MQ=255) ccccTCGTTTCGCATATTCGCGCCGAGTGTTCGGCGTTGGCGTCCAGGTTGGATcttctt                                                       >  1:1099271/1‑60 (MQ=255) ccccTCGTTTCGCATATTCGCGCCGAGTGTTCGGCGTTGGCGTCCAGGTTGGATCTTCTTGttt                                                   >  1:1756591/1‑64 (MQ=255) ccccTCGTTTCGCATATTCGCGCCGAGTGTTCGGCGTTGGCGTCCAGGTTGGATCTTCTTGatt                                                   >  1:269434/1‑64 (MQ=255) ccccTCGTTTCGCATATTCGCGCCGAGTGTTCGGCGTTGGCGTCCAGGTTGGATCTTCTTGTTTa                                                  >  1:2688445/1‑65 (MQ=255) ccccTCGTTTCGCATATTCGCGCCGAGTGTTCGGCGTTGGCGTCCAGGTTGGATCTTCTTGTTTa                                                  >  1:610541/1‑65 (MQ=255) ccccTCGTTTCGCATATTCGCGCCGAGTGTTCGGCGTTGGCGTCCAGGTTGGATCTTCTTGTTTa                                                  >  1:417170/1‑65 (MQ=255) ccccTCGTTTCGCATATTCGCGCCGAGTGTTCGGCGTTGGCGTCCAGGTTGGATCTTCTTGTTTa                                                  >  1:3071676/1‑65 (MQ=255) ccccTCGTTTCGCATATTCGCGCCGAGTGTTCGGCGTTGGCGTCCAGGTTGGATCTTCTTGTTTa                                                  >  1:2947503/1‑65 (MQ=255) ccccTCGTTTCGCATATTCGCGCCGAGTGTTCGGCGTTGGCGTCCAGGTTGGATCTTCTTGTTTa                                                  >  1:2818197/1‑65 (MQ=255) ccccTCGTTTCGCATATTCGCGCCGAGTGTTCGGCGTTGGCGTCCAGGTTGGATCTTCTTGTTTa                                                  >  1:2801197/1‑65 (MQ=255) ccccTCGTTTCGCATATTCGCGCCGAGTGTTCGGCGTTGGCGTCCAGGTTGGATCTTCTTGTTTa                                                  >  1:2766919/1‑65 (MQ=255) ccccTCGTTTCGCATATTCGCGCCGAGTGTTCGGCGTTGGCGTCCAGGTTGGATCTTCTTGTTTa                                                  >  1:2762857/1‑65 (MQ=255) ccccTCGTTTCGCATATTCGCGCCGAGTGTTCGGCGTTGGCGTCCAGGTTGGATCTTCTTGTTTa                                                  >  1:2323761/1‑65 (MQ=255) ccccTCGTTTCGCATATTCGCGCCGAGTGTTCGGCGTTGGCGTCCAGGTTGGATCTTCTTGTTTa                                                  >  1:2250923/1‑65 (MQ=255) ccccTCGTTTCGCATATTCGCGCCGAGTGTTCGGCGTTGGCGTCCAGGTTGGATCTTCTTGTTTa                                                  >  1:2019225/1‑65 (MQ=255) ccccTCGTTTCGCATATTCGCGCCGAGTGTTCGGCGTTGGCGTCCAGGTTGGATCTTCTTGTTTa                                                  >  1:1744458/1‑65 (MQ=255) ccccTCGTTTCGCATATTCGCGCCGAGTGTTCGGCGTTGGCGTCCAGGTTGGATCTTCTTGTTTa                                                  >  1:1708381/1‑65 (MQ=255)      cGTTTCGCATATTCGCGCCGAGTGTTCGGCGTTGGCGTCCAGGTT‑GATCTTCTTGTTTACGTTCa                                            <  1:768019/65‑1 (MQ=255)         ttCGCATATTCGCGCCGAGTGTTCGGCGTTGGCGTCCAGGTTGGATCTTCTTGTTTACGTTCa                                            <  1:2497113/63‑1 (MQ=255)         ttCGCATATTCGCGCCGAGTGTTCGGCGTTGGCGTCCAGGTTGGATCTTCTTGTTTACGTTCa                                            <  1:2993146/63‑1 (MQ=255)         ttCGCATATTCGCGCCGAGTGTTCGGCGTTGGCGTCCAGGTTGGATCTTCTTGTTTACGTTCa                                            <  1:1414881/63‑1 (MQ=255)         ttCGCATATTCGCGCCGAGTGTTCGGCGTTGGCGTCCAGGTTGGATCTTCTTGTTTACGTTCa                                            <  1:457173/63‑1 (MQ=255)         ttCGCATATTCGCGCCGAGTGTTCGGCGTTGGCGTCCAGGTTGGATCTTCTTGTTTACGTTCa                                            <  1:1375908/63‑1 (MQ=255)                           gtgtTCGGCGTTGGCGTCCAGGTTGGATCTTCTTGTTTACGTTCa                                            <  1:1501359/45‑1 (MQ=255)                                                 ttGGATCTTCTTGTTTACGTTCAACGGTAGTCACCCAGTTACGCGGGGTTTCTCGCCCAGCCTgg  >  1:1312563/1‑65 (MQ=255)                                                 ttGGATCTTCTTGTTTACGTTCAACGGTAGTCACCCAGTTACGCGGGGTTTCTCGCCCAGCCTg   >  1:2492241/1‑64 (MQ=255)                                                 ttGGATCTTCTTGTTTACGTTCAACGGTAGTCACCCAGTTACGCGGGGTTTCTCGCCCAGCCTg   >  1:222078/1‑64 (MQ=255)                                                 ttGGATCTTCTTGTTTACGTTCAACGGTAGTCACCCAGTTACGCGGGGTTTCTCGCCCAGCCTg   >  1:545938/1‑64 (MQ=255)                                                      |                                                            CCCCTCGTTTCGCATATTCGCGCCGAGTGTTCGGCGTTGGCGTCCAGGTTGGAGCTTCTTGTTTACGTTCAACGGTAGTCACCCAGTTACGCGGGGTTTCTCGCCCAGCCTGG  >  W3110S.gb/2064974‑2065086

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑