Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,077,011	G→A	L32L (CTG→CTA)	yeeR →	predicted membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,077,011	0	G	A	100.0%	88.4 / NA	27	L32L (CTG→CTA)	yeeR	predicted membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/18);  total (9/18)

AGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGCCTTACTGGCTG  >  W3110S.gb/2076954‑2077057                                                          |                                               aGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTAGCAggg                                          <  1:359122/64‑1 (MQ=255)                  gCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTAGCAGGGCTCATATTGCTGTGTCTg                        >  1:1514819/1‑65 (MQ=255)                  gCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTAGCAGGGCTCATATTGCTGTGTCTg                        >  1:362235/1‑65 (MQ=255)                  gCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTAGCAGGGCTCATATTGCTGTGTCTg                        >  1:2566841/1‑65 (MQ=255)                  gCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTAGCAGGGCTCATATTGCTGTGTCTg                        >  1:2536927/1‑65 (MQ=255)                  gCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTAGCAGGGCTCATATTGCTGTGTCTg                        >  1:238517/1‑65 (MQ=255)                  gCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTAGCAGGGCTCATATTGCTGTGTCTg                        >  1:1986497/1‑65 (MQ=255)                  gCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTAGCAGGGCTCATATTGCTGTGTCTg                        >  1:1930863/1‑65 (MQ=255)                  gCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTAGCAGGGCTCATATTGCTGTGTCTg                        >  1:1884365/1‑65 (MQ=255)                  gCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTAGCAGGGCTCATATTGCTGTGTCTg                        >  1:1303968/1‑65 (MQ=255)                                        cAGCACGGCTTCTGCGCTAGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGCCTTActggctg  <  1:766754/65‑1 (MQ=255)                                        cAGCACGGCTTCTGCGCTAGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGCCTTActggctg  <  1:2471343/65‑1 (MQ=255)                                        cAGCACGGCTTCTGCGCTAGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGCCTTActggctg  <  1:2704663/65‑1 (MQ=255)                                         aGCACGGCTTCTGCGCTAGCAGGGCTCATATTGCTGTGTCTGTTcggcccg               <  1:771330/51‑1 (MQ=255)                                         aGCACGGCTTCTGCGCTAGCAGGGCTCATATTGCTGTGTCTGTTcggcccg               <  1:683990/51‑1 (MQ=255)                                         aGCACGGCTTCTGCGCTAGCAGGGCTCATATTGCTGTGTCTGTTcggcccg               <  1:630456/51‑1 (MQ=255)                                         aGCACGGCTTCTGCGCTAGCAGGGCTCATATTGCTGTGTCTGTTcggcccg               <  1:533990/51‑1 (MQ=255)                                         aGCACGGCTTCTGCGCTAGCAGGGCTCATATTGCTGTGTCTGTTcggcccg               <  1:844315/51‑1 (MQ=255)                                         aGCACGGCTTCTGCGCTAGCAGGGCTCATATTGCTGTGTCTGTTcggcccg               <  1:2952801/51‑1 (MQ=255)                                         aGCACGGCTTCTGCGCTAGCAGGGCTCATATTGCTGTGTCTGTTcggcccg               <  1:2723479/51‑1 (MQ=255)                                         aGCACGGCTTCTGCGCTAGCAGGGCTCATATTGCTGTGTCTGTTcggcccg               <  1:2680691/51‑1 (MQ=255)                                         aGCACGGCTTCTGCGCTAGCAGGGCTCATATTGCTGTGTCTGTTcggcccg               <  1:2596477/51‑1 (MQ=255)                                         aGCACGGCTTCTGCGCTAGCAGGGCTCATATTGCTGTGTCTGTTcggcccg               <  1:2568495/51‑1 (MQ=255)                                         aGCACGGCTTCTGCGCTAGCAGGGCTCATATTGCTGTGTCTGTTcggcccg               <  1:2179979/51‑1 (MQ=255)                                         aGCACGGCTTCTGCGCTAGCAGGGCTCATATTGCTGTGTCTGTTcggcccg               <  1:2009180/51‑1 (MQ=255)                                         aGCACGGCTTCTGCGCTAGCAGGGCTCATATTGCTGTGTCTGTTcggcccg               <  1:1852035/51‑1 (MQ=255)                                          gCACGGCTTCTGCGCTAGCAGGGCTCATATTGCTGTGTCTGTTcggcccg               <  1:2961602/50‑1 (MQ=255)                                                          |                                               AGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGCCTTACTGGCTG  >  W3110S.gb/2076954‑2077057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑