Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,096,084	G→A	E160E (GAG→GAA)	hisB →	fused histidinol‑phosphatase and imidazoleglycerol‑phosphate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,096,084	0	G	A	97.2%	107.7 / ‑6.0	36	E160E (GAG→GAA)	hisB	fused histidinol‑phosphatase and imidazoleglycerol‑phosphate dehydratase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (27/8);  minor base C (1/0);  total (28/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGT  >  W3110S.gb/2096038‑2096119                                               |                                    gTTTACGCTACGCCCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGAc                   >  1:1572317/1‑65 (MQ=255) gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGACCAACTCACCAGACGTGAc                   >  1:1320248/1‑65 (MQ=255) gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACa                                   >  1:2999978/1‑49 (MQ=255) gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACGAGACGTGAc                   >  1:1413472/1‑65 (MQ=255) gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGAc                   >  1:39995/1‑65 (MQ=255) gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGAc                   >  1:2401555/1‑65 (MQ=255) gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGAc                   >  1:2461209/1‑65 (MQ=255) gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGAc                   >  1:2976044/1‑65 (MQ=255) gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGAc                   >  1:3010520/1‑65 (MQ=255) gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGAc                   >  1:373294/1‑65 (MQ=255) gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGAc                   >  1:385284/1‑65 (MQ=255) gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGAc                   >  1:1027593/1‑65 (MQ=255) gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGAc                   >  1:402421/1‑65 (MQ=255) gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGAc                   >  1:610742/1‑65 (MQ=255) gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGAc                   >  1:639643/1‑65 (MQ=255) gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGAc                   >  1:836678/1‑65 (MQ=255) gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGAc                   >  1:879771/1‑65 (MQ=255) gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGAc                   >  1:894762/1‑65 (MQ=255) gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGAc                   >  1:2255052/1‑65 (MQ=255) gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGAc                   >  1:1002320/1‑65 (MQ=255) gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGAc                   >  1:1331050/1‑65 (MQ=255) gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGAc                   >  1:1434053/1‑65 (MQ=255) gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGAc                   >  1:1486825/1‑65 (MQ=255) gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGAc                   >  1:1655985/1‑65 (MQ=255) gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGAc                   >  1:1741674/1‑65 (MQ=255) gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGAc                   >  1:2096389/1‑65 (MQ=255) gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGAc                   >  1:2160703/1‑65 (MQ=255) gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGAc                   >  1:2378752/1‑65 (MQ=255)                  gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:1822010/65‑1 (MQ=255)                  gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:2954696/65‑1 (MQ=255)                  gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:405386/65‑1 (MQ=255)                  gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:1357682/65‑1 (MQ=255)                  gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:2658624/65‑1 (MQ=255)                  gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:1305800/65‑1 (MQ=255)                  gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:944757/65‑1 (MQ=255)                                    aTGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:1235653/47‑1 (MQ=255)                                               |                                    GTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGT  >  W3110S.gb/2096038‑2096119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑