Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,215,186	A→G	V115V (GTT→GTC)	yehS ←	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,215,186	0	A	G	100.0%	56.4 / NA	16	V115V (GTT→GTC)	yehS	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/16);  total (0/16)

TTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAAACGCGGAACTGCTGTTCGG  >  W3110S.gb/2215141‑2215205                                              |                    tttATGATCCGGTGCGCGCATCATCGCTTTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCgg  <  1:3064022/65‑1 (MQ=255) tttATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCgg  <  1:1040518/65‑1 (MQ=255) tttATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCgg  <  1:1051204/65‑1 (MQ=255) tttATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCgg  <  1:1175513/65‑1 (MQ=255) tttATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCgg  <  1:147561/65‑1 (MQ=255) tttATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCgg  <  1:2397775/65‑1 (MQ=255) tttATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCgg  <  1:2503212/65‑1 (MQ=255) tttATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCgg  <  1:253201/65‑1 (MQ=255) tttATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCgg  <  1:2958127/65‑1 (MQ=255) tttATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCgg  <  1:45205/65‑1 (MQ=255) tttATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCgg  <  1:580067/65‑1 (MQ=255) tttATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCgg  <  1:792773/65‑1 (MQ=255) tttATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCgg  <  1:89911/65‑1 (MQ=255) tttATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCgg  <  1:993537/65‑1 (MQ=255) tttATGATCCGGAGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCgg  <  1:1262209/65‑1 (MQ=255)  ttATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCgg  <  1:1325761/64‑1 (MQ=255)                                              |                    TTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAAACGCGGAACTGCTGTTCGG  >  W3110S.gb/2215141‑2215205

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑