Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,268,596	T→C	T12A (ACT→GCT)	yeiW ←	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,268,596	0	T	C	100.0%	206.8 / NA	55	T12A (ACT→GCT)	yeiW	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (43/12);  total (43/12)

CGGGCTTGCCATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCGCAACCCGGACGGCATTCCATACGTTGTTCTCTTAAGGTT  >  W3110S.gb/2268545‑2268648                                                    |                                                     cGGGCTTGCCATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGCGCAACACGcccc                                          >  1:1069656/1‑64 (MQ=255) cGGGCTTGCCATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGCGCAACACGcccc                                          >  1:2516732/1‑64 (MQ=255) cGGGCTTGCCATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGCGCAACACGcccc                                          >  1:2395241/1‑64 (MQ=255) cGGGCTTGCCATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGCGCAACACGcccc                                          >  1:2369075/1‑64 (MQ=255) cGGGCTTGCCATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGCGCAACACGcccc                                          >  1:2345076/1‑64 (MQ=255) cGGGCTTGCCATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGCGCAACACGcccc                                          >  1:1225780/1‑64 (MQ=255) cGGGCTTGCCATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGCGCAACACGcccc                                          >  1:1925318/1‑64 (MQ=255) cGGGCTTGCCATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGCGCAACACGcccc                                          >  1:2922661/1‑64 (MQ=255) cGGGCTTGCCATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGCGCAACACGcccc                                          >  1:1680948/1‑64 (MQ=255) cGGGCTTGCCATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGCGCAACACGcccc                                          >  1:1622059/1‑64 (MQ=255) cGGGCTTGCCATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGCGCAACACGcccc                                          >  1:2697156/1‑64 (MQ=255) cGGGCTTGCCATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGCGCAACACGcccc                                          >  1:1448798/1‑64 (MQ=255) cGGGCTTGCCATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGCGCAACACGccc                                           >  1:254315/1‑63 (MQ=255) cGGGCTTGCCATCTGGCATACCGGGAATAGGGCTGGAAAATGAAGGGGCAGCGCAACACGcccc                                          >  1:2845467/1‑64 (MQ=255) cGGGCTTGCCATATGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGCGCAACACGcccc                                          >  1:712830/1‑64 (MQ=255)         ccATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCTGCGCAACACGCCCCGCAACCCgg                                 >  1:1978094/1‑65 (MQ=255)         ccATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGCGCAacac                                               >  1:1342020/1‑51 (MQ=255)         ccATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGCGCAACACg                                              >  1:1556933/1‑52 (MQ=255)         ccATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGCGCAACACGCCCCGCAACCCgg                                 >  1:2178075/1‑65 (MQ=255)         ccATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGCGCAACACGCCCCGCAACCCgg                                 >  1:2535689/1‑65 (MQ=255)         ccATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGCGCAACACGCCCCGCAACCCgg                                 >  1:2520451/1‑65 (MQ=255)         ccATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGCGCAACACGCCCCGCAACCCgg                                 >  1:1181081/1‑65 (MQ=255)         ccATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGCGCAACACGCCCCGCAACCCgg                                 >  1:2420794/1‑65 (MQ=255)         ccATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGCGCAACACGCCCCGCAACCCgg                                 >  1:1392035/1‑65 (MQ=255)         ccATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGCGCAACACGCCCCGCAACCCgg                                 >  1:2354127/1‑65 (MQ=255)         ccATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGCGCAACACGCCCCGCAACCCgg                                 >  1:503930/1‑65 (MQ=255)         ccATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGCGCAACACGCCCCGCAACCCgg                                 >  1:2277528/1‑65 (MQ=255)         ccATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGCGCAACACGCCCCGCAACCCgg                                 >  1:2273944/1‑65 (MQ=255)         ccATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGCGCAACACGCCCCGCAACCCgg                                 >  1:2263058/1‑65 (MQ=255)         ccATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGCGCAACACGCCCCGCAACCCgg                                 >  1:2209525/1‑65 (MQ=255)         ccATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGCGCAACACGCCCCGCAACCCgg                                 >  1:1334850/1‑65 (MQ=255)         ccATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGCGCAACACGCCCCGCAACCCgg                                 >  1:209953/1‑65 (MQ=255)         ccATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGCGCAACACGCCCCGCAACCCgg                                 >  1:1341392/1‑65 (MQ=255)         ccATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGCGCAACACGCCCCGCAACCCgg                                 >  1:255484/1‑65 (MQ=255)         ccATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGCGCAACACGCCCCGCAACCCgg                                 >  1:195884/1‑65 (MQ=255)         ccATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGCGCAACACGCCCCGCAACCCgg                                 >  1:346207/1‑65 (MQ=255)         ccATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGCGCAACACGCCCCGCAACCCgg                                 >  1:1915520/1‑65 (MQ=255)         ccATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGCGCAACACGCCCCGCAACCCgg                                 >  1:1909317/1‑65 (MQ=255)         ccATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGCGCAACACGCCCCGCAACCCgg                                 >  1:1869336/1‑65 (MQ=255)         ccATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGCGCAACACGCCCCGCAACCCgg                                 >  1:1810322/1‑65 (MQ=255)         ccATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGCGCAACACGCCCCGCAACCCgg                                 >  1:255734/1‑65 (MQ=255)         ccATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGCGCAACACGCCCCGCAACCCgg                                 >  1:2617654/1‑65 (MQ=255)         ccATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGCGCAACACGCCCCGCAACCCg                                  >  1:741491/1‑64 (MQ=255)                                      aaTTGAAGGGGCAGCGCAACACGCCCCGCAACCCGGACGGCATTCCATACGTTGTTCTCTTAAg     <  1:878255/64‑1 (MQ=255)                                      aaTTGAAGGGGCAGCGCAACACGCCCCGCAACCCGGACGGCATTCCATACGTTGTTCTCTTAAg     <  1:1801886/64‑1 (MQ=255)                                        ttGAAGGGGCAGCGCAACACGCCCCGCAACCCGGACGGCATTCCATACGTTGTTCTCTTAAGGtt  <  1:1960506/65‑1 (MQ=255)                                        ttGAAGGGGCAGCGCAACACGCCCCGCAACCCGGACGGCATTCCATACGTTGTTCTCTTAAGGtt  <  1:1711816/65‑1 (MQ=255)                                        ttGAAGGGGCAGCGCAACACGCCCCGCAACCCGGACGGCATTCCATACGTTGTTCTCTTAAGGtt  <  1:1525088/65‑1 (MQ=255)                                        ttGAAGGGGCAGCGCAACACGCCCCGCAACCCGGACGGCATTCCATACGTTGTTCTCTTAAGGtt  <  1:1244884/65‑1 (MQ=255)                                        ttGAAGGGGCAGCGCAACACGCCCCGCAACCCGGACGGCATTCCATACGTTGTTCTCTTAAGGtt  <  1:1240845/65‑1 (MQ=255)                                        ttGAAGGGGCAGCGCAACACGCCCCGCAACCCGGACGGCATTCCATACGTTGTTCTCTTAAGGtt  <  1:122717/65‑1 (MQ=255)                                        ttGAAGGGGCAGCGCAACACGCCCCGCAACCCGGACGGCATTCCATACGTTGTTCTCTTAAGGtt  <  1:1210499/65‑1 (MQ=255)                                        ttGAAGGGGCAGCGCAACACGCCCCGCAACCCGGACGGCATTCCATACGTTGTTCTCTTAAGGtt  <  1:1186448/65‑1 (MQ=255)                                        ttGAAGGGGCAGCGCAACACGCCCCGCAACCCGGACGGCATTCCATACGTTGTTCTCTTAAGGtt  <  1:1185829/65‑1 (MQ=255)                                        ttGAAGGGGCAGCGCAACACGCCCCGCAACCCGGACGGCATTCCATACGTTGTTCTCTTAAGGtt  <  1:1027210/65‑1 (MQ=255)                                                    |                                                     CGGGCTTGCCATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCGCAACCCGGACGGCATTCCATACGTTGTTCTCTTAAGGTT  >  W3110S.gb/2268545‑2268648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑