Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,271,945	A→G	R261R (CGA→CGG)	yeiR →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,271,945	0	A	G	100.0%	57.5 / NA	17	R261R (CGA→CGG)	yeiR	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/0);  total (17/0)

AATGGGCGCGACTTGCACCGGTGGAACGCGTCAAAGGCGTGCTGCGTATTCCCGAAGGGCTGGT  >  W3110S.gb/2271935‑2271998           |                                                      aaTGGGCGCGGCTTGCACCGGTGGAACGCGTCAAAGGCGTGCTGCGTATTCCCGAAGGGCTgg   >  1:1300565/1‑63 (MQ=255) aaTGGGCGCGGCTTGCACCGGTGGAACGCGTCAAAGGCGTGCTGCGTATTCCCGAAGGGCTGGt  >  1:2860728/1‑64 (MQ=255) aaTGGGCGCGGCTTGCACCGGTGGAACGCGTCAAAGGCGTGCTGCGTATTCCCGAAGGGCTGGt  >  1:943687/1‑64 (MQ=255) aaTGGGCGCGGCTTGCACCGGTGGAACGCGTCAAAGGCGTGCTGCGTATTCCCGAAGGGCTGGt  >  1:780282/1‑64 (MQ=255) aaTGGGCGCGGCTTGCACCGGTGGAACGCGTCAAAGGCGTGCTGCGTATTCCCGAAGGGCTGGt  >  1:769670/1‑64 (MQ=255) aaTGGGCGCGGCTTGCACCGGTGGAACGCGTCAAAGGCGTGCTGCGTATTCCCGAAGGGCTGGt  >  1:588483/1‑64 (MQ=255) aaTGGGCGCGGCTTGCACCGGTGGAACGCGTCAAAGGCGTGCTGCGTATTCCCGAAGGGCTGGt  >  1:48045/1‑64 (MQ=255) aaTGGGCGCGGCTTGCACCGGTGGAACGCGTCAAAGGCGTGCTGCGTATTCCCGAAGGGCTGGt  >  1:291213/1‑64 (MQ=255) aaTGGGCGCGGCTTGCACCGGTGGAACGCGTCAAAGGCGTGCTGCGTATTCCCGAAGGGCTGGt  >  1:2883633/1‑64 (MQ=255) aaTGGGCGCGGCTTGCACCGGTGGAACGCGTCAAAGGCGTGCTGCGTATTCCCGAAGGGCTGGt  >  1:2812381/1‑64 (MQ=255) aaTGGGCGCGGCTTGCACCGGTGGAACGCGTCAAAGGCGTGCTGCGTATTCCCGAAGGGCTGGt  >  1:2641181/1‑64 (MQ=255) aaTGGGCGCGGCTTGCACCGGTGGAACGCGTCAAAGGCGTGCTGCGTATTCCCGAAGGGCTGGt  >  1:1822238/1‑64 (MQ=255) aaTGGGCGCGGCTTGCACCGGTGGAACGCGTCAAAGGCGTGCTGCGTATTCCCGAAGGGCTGGt  >  1:1799362/1‑64 (MQ=255) aaTGGGCGCGGCTTGCACCGGTGGAACGCGTCAAAGGCGTGCTGCGTATTCCCGAAGGGCTGGt  >  1:178333/1‑64 (MQ=255) aaTGGGCGCGGCTTGCACCGGTGGAACGCGTCAAAGGCGTGCTGCGTATTCCCGAAGGGCTGGt  >  1:1576907/1‑64 (MQ=255) aaTGGGCGCGGCTTGCACCGGTGGAACGCGTCAAAGGCGTGCTGCGTATTCCCGAAGGGCTGGt  >  1:1313100/1‑64 (MQ=255) aaTGGGCGCGGCTTGCACCAGTGGAACGCGTCAAAGGCGTGCTGCGTATTCCCGAAGGGCTGGt  >  1:340083/1‑64 (MQ=255)           |                                                      AATGGGCGCGACTTGCACCGGTGGAACGCGTCAAAGGCGTGCTGCGTATTCCCGAAGGGCTGGT  >  W3110S.gb/2271935‑2271998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑