Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,274,249	A→G	T64A (ACT→GCT)	rtn →	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,274,249	0	A	G	100.0%	201.8 / NA	54	T64A (ACT→GCT)	rtn	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/34);  total (20/34)

CACACTGATTACCGACGTACAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCACTACTGACCGGCTCCAGCCGCTGACCTTAGATACCTGCCAGCAAGCT  >  W3110S.gb/2274191‑2274296                                                           |                                                cacacTGATTACCGACGTACAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCgctact                                            >  1:345955/1‑64 (MQ=255) cacacTGATTACCGACGTACAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTg                                           >  1:1052736/1‑65 (MQ=255) cacacTGATTACCGACGTACAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTg                                           >  1:2642483/1‑65 (MQ=255) cacacTGATTACCGACGTACAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTg                                           >  1:1962785/1‑65 (MQ=255) cacacTGATTACCGACGTACAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTg                                           >  1:721892/1‑65 (MQ=255) cacacTGATTACCGACGTACAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTg                                           >  1:67672/1‑65 (MQ=255) cacacTGATTACCGACGTACAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTg                                           >  1:1539468/1‑65 (MQ=255)         ttACCGACGTACAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCgctact                                            >  1:1610458/1‑56 (MQ=255)         ttACCGACGTACAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGc                                     >  1:568812/1‑63 (MQ=255)         ttACCGACGTACAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGc                                     >  1:2509895/1‑63 (MQ=255)         ttACCGACGTACAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGc                                     >  1:2393749/1‑63 (MQ=255)         ttACCGACGTACAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGc                                     >  1:2576639/1‑63 (MQ=255)         ttACCGACGTACAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGc                                     >  1:26523/1‑63 (MQ=255)         ttACCGACGTACAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGc                                     >  1:2861945/1‑63 (MQ=255)         ttACCGACGTACAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGc                                     >  1:587875/1‑63 (MQ=255)         ttACCGACGTACAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGc                                     >  1:1478571/1‑63 (MQ=255)         ttACCGACGTACAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGc                                     >  1:767155/1‑63 (MQ=255)         ttACCGACGTACAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGc                                     >  1:783406/1‑63 (MQ=255)                    cAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGAc                        <  1:945645/65‑1 (MQ=255)                    cAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGAc                        <  1:936878/65‑1 (MQ=255)                    cAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGAc                        <  1:92261/65‑1 (MQ=255)                    cAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGAc                        <  1:2587992/65‑1 (MQ=255)                    cAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGAc                        <  1:870939/65‑1 (MQ=255)                    cAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGAc                        <  1:83668/65‑1 (MQ=255)                    cAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGAc                        <  1:2812418/65‑1 (MQ=255)                    cAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGAc                        <  1:2816520/65‑1 (MQ=255)                    cAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGAc                        <  1:2945682/65‑1 (MQ=255)                    cAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGAc                        <  1:302027/65‑1 (MQ=255)                    cAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGAc                        <  1:3051442/65‑1 (MQ=255)                    cAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGAc                        <  1:756843/65‑1 (MQ=255)                    cAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGAc                        <  1:1043216/65‑1 (MQ=255)                    cAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGAc                        <  1:2343609/65‑1 (MQ=255)                    cAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGAc                        <  1:1025895/65‑1 (MQ=255)                    cAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGAc                        <  1:1108470/65‑1 (MQ=255)                    cAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGAc                        <  1:111694/65‑1 (MQ=255)                    cAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGAc                        <  1:1187381/65‑1 (MQ=255)                    cAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGAc                        <  1:1214174/65‑1 (MQ=255)                    cAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGAc                        <  1:1451785/65‑1 (MQ=255)                    cAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGAc                        <  1:1455382/65‑1 (MQ=255)                    cAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGAc                        <  1:1484891/65‑1 (MQ=255)                    cAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGAc                        <  1:1493936/65‑1 (MQ=255)                    cAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGAc                        <  1:1523335/65‑1 (MQ=255)                    cAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGAc                        <  1:1559527/65‑1 (MQ=255)                    cAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGAc                        <  1:1582178/65‑1 (MQ=255)                    cAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGAc                        <  1:167889/65‑1 (MQ=255)                    cAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGAc                        <  1:1724622/65‑1 (MQ=255)                    cAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGAc                        <  1:2039891/65‑1 (MQ=255)                    cAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGAc                        <  1:2074348/65‑1 (MQ=255)                    cAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGAc                        <  1:2128322/65‑1 (MQ=255)                    cAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGAc                        <  1:2142797/65‑1 (MQ=255)                    cAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGAc                        <  1:2146949/65‑1 (MQ=255)                    cAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGAc                        <  1:2214937/65‑1 (MQ=255)                          tATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGACCTTAGa                  >  1:1939845/1‑65 (MQ=255)                                          ttGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGACCTTAGATACCTGCCAGCAAGCt  >  1:529414/1‑65 (MQ=255)                                          ttGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGACCTTAGATACCTGCCAGCAAGCt  >  1:271593/1‑65 (MQ=255)                                          ttGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGACCTTAGATACCTGCCAGCAAGCt  >  1:2386867/1‑65 (MQ=255)                                                           |                                                CACACTGATTACCGACGTACAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCACTACTGACCGGCTCCAGCCGCTGACCTTAGATACCTGCCAGCAAGCT  >  W3110S.gb/2274191‑2274296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑