Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	376,160	G→T	Q55H (CAG→CAT)	yaiL →	nucleoprotein/polynucleotide‑associated enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	376,160	0	G	T	100.0%	40.6 / NA	13	Q55H (CAG→CAT)	yaiL	nucleoprotein/polynucleotide‑associated enzyme
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (13/0);  total (13/0)

GCGGCGGTAGAAGAAAATAAAAAGGCACAGCTTGAGCGTGATAAACAGCTTAGCGAACAGCAAAAACAAGCGGCGTTGGCGAAAGAATATAAAGCTCAG  >  W3110S.gb/376113‑376211                                                |                                                    gcggcgGTAGAAGAAAATAAAAAGGCACAGCTTGAGCGTGATAAACATCTTAGCGAACAGCaaa                                     >  1:167153/1‑64 (MQ=255) gcggcgGTAGAAGAAAATAAAAAGGCACAGCTTGAGCGTGATAAACATCTTAGCGAACAGCaaa                                     >  1:2357619/1‑64 (MQ=255) gcggcgGTAGAAGAAAATAAAAAGGCACAGCTTGAGCGTGATAAACATCTTAGCGAACAGCaaa                                     >  1:2409247/1‑64 (MQ=255) gcggcgGTAGAAGAAAATAAAAAGGCACAGCTTGAGCGTGATAAACATCTTAGCGAACAGCaaa                                     >  1:2549808/1‑64 (MQ=255) gcggcgGTAGAAGAAAATAAAAAGGCACAGCTTGAGCGTGATAAACATCTTAGCGAACAGCaaa                                     >  1:487221/1‑64 (MQ=255)                                    gCGTGATAAACATCTTAGCGAACAGCAAAAACAAGCGGCGTTGGCGAAAGAATATAAAGCTCAg  >  1:1107991/1‑64 (MQ=255)                                    gCGTGATAAACATCTTAGCGAACAGCAAAAACAAGCGGCGTTGGCGAAAGAATATAAAGCTCAg  >  1:1180591/1‑64 (MQ=255)                                    gCGTGATAAACATCTTAGCGAACAGCAAAAACAAGCGGCGTTGGCGAAAGAATATAAAGCTCAg  >  1:1590426/1‑64 (MQ=255)                                    gCGTGATAAACATCTTAGCGAACAGCAAAAACAAGCGGCGTTGGCGAAAGAATATAAAGCTCAg  >  1:2210648/1‑64 (MQ=255)                                    gCGTGATAAACATCTTAGCGAACAGCAAAAACAAGCGGCGTTGGCGAAAGAATATAAAGCTCAg  >  1:271443/1‑64 (MQ=255)                                    gCGTGATAAACATCTTAGCGAACAGCAAAAACAAGCGGCGTTGGCGAAAGAATATAAAGCTCAg  >  1:2795392/1‑64 (MQ=255)                                    gCGTGATAAACATCTTAGCGAACAGCAAAAACAAGCGGCGTTGGCGAAAGAATATAAAGCTCAg  >  1:3048988/1‑64 (MQ=255)                                    gCGTGATAAACATCTTAGCGAACAGCAAAAACAAGCGGCGTTGGCGAAAGAATATAAAGCTCAg  >  1:605804/1‑64 (MQ=255)                                                |                                                    GCGGCGGTAGAAGAAAATAAAAAGGCACAGCTTGAGCGTGATAAACAGCTTAGCGAACAGCAAAAACAAGCGGCGTTGGCGAAAGAATATAAAGCTCAG  >  W3110S.gb/376113‑376211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑