Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,298,568 T→C E49E (GAA→GAG ccmE ← periplasmic heme chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,298,5680TC100.0% 248.0 / NA 67E49E (GAA→GAGccmEperiplasmic heme chaperone
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/48);  total (19/48)

TCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTTTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGAGCGCAG  >  W3110S.gb/2298522‑2298627
                                              |                                                           
tCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAgaga                                           >  1:2722619/1‑65 (MQ=255)
tCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAgaga                                           >  1:2444896/1‑65 (MQ=255)
tCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAgaga                                           >  1:1918909/1‑65 (MQ=255)
tCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAgaga                                           >  1:2471291/1‑65 (MQ=255)
tCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAgaga                                           >  1:1837711/1‑65 (MQ=255)
tCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAgaga                                           >  1:2041350/1‑65 (MQ=255)
tCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAgaga                                           >  1:1825923/1‑65 (MQ=255)
tCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAgaga                                           >  1:2574241/1‑65 (MQ=255)
tCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAgaga                                           >  1:1536603/1‑65 (MQ=255)
tCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAgaga                                           >  1:2171900/1‑65 (MQ=255)
tCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAgaga                                           >  1:2736850/1‑65 (MQ=255)
tCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAgaga                                           >  1:3015017/1‑65 (MQ=255)
tCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAgaga                                           >  1:3105688/1‑65 (MQ=255)
tCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAgaga                                           >  1:364408/1‑65 (MQ=255)
tCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAgaga                                           >  1:226559/1‑65 (MQ=255)
tCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAgaga                                           >  1:440379/1‑65 (MQ=255)
tCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAgaga                                           >  1:729554/1‑65 (MQ=255)
tCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCAATTGCTGAGTCTCACGCTTGCCGTAgaga                                           >  1:2296740/1‑65 (MQ=255)
tCCCGCCAACGCGCAGACGCAGACCGACTTCCGGCATTTGCTGAGTCTCACGCTTg                                                    >  1:246844/1‑56 (MQ=255)
                        cGACTTCCGGCATTTGCTGAGTCTCACGTTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga                   <  1:773839/65‑1 (MQ=255)
                        cGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga                   <  1:2286230/65‑1 (MQ=255)
                        cGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga                   <  1:2314744/65‑1 (MQ=255)
                        cGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga                   <  1:2375466/65‑1 (MQ=255)
                        cGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga                   <  1:1039771/65‑1 (MQ=255)
                        cGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga                   <  1:2514717/65‑1 (MQ=255)
                        cGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga                   <  1:2651474/65‑1 (MQ=255)
                        cGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga                   <  1:2720282/65‑1 (MQ=255)
                        cGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga                   <  1:3019469/65‑1 (MQ=255)
                        cGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga                   <  1:3107112/65‑1 (MQ=255)
                        cGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga                   <  1:389723/65‑1 (MQ=255)
                        cGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga                   <  1:681137/65‑1 (MQ=255)
                        cGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga                   <  1:2247837/65‑1 (MQ=255)
                        cGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga                   <  1:1289575/65‑1 (MQ=255)
                        cGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga                   <  1:1143484/65‑1 (MQ=255)
                        cGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga                   <  1:1490407/65‑1 (MQ=255)
                        cGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga                   <  1:1832801/65‑1 (MQ=255)
                        cGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga                   <  1:1900848/65‑1 (MQ=255)
                        cGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga                   <  1:2162807/65‑1 (MQ=255)
                        cGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga                   <  1:2246869/65‑1 (MQ=255)
                                      tGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGt                             <  1:78869/41‑1 (MQ=255)
                                      tGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGt                             <  1:802103/41‑1 (MQ=255)
                                      tGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGt                             <  1:1056186/41‑1 (MQ=255)
                                      tGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGt                             <  1:567725/41‑1 (MQ=255)
                                      tGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGt                             <  1:552388/41‑1 (MQ=255)
                                      tGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGt                             <  1:42749/41‑1 (MQ=255)
                                      tGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGt                             <  1:413546/41‑1 (MQ=255)
                                      tGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGt                             <  1:1128302/41‑1 (MQ=255)
                                      tGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGt                             <  1:337409/41‑1 (MQ=255)
                                      tGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGt                             <  1:1204118/41‑1 (MQ=255)
                                      tGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGt                             <  1:1302575/41‑1 (MQ=255)
                                      tGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGt                             <  1:1860953/41‑1 (MQ=255)
                                      tGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGt                             <  1:1499048/41‑1 (MQ=255)
                                      tGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGt                             <  1:1771860/41‑1 (MQ=255)
                                      tGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGt                             <  1:3089163/41‑1 (MQ=255)
                                       gCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGt                             <  1:1319306/40‑1 (MQ=255)
                                          gAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGagcgcag  <  1:1109374/64‑1 (MQ=255)
                                          gAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGagcgcag  <  1:965107/64‑1 (MQ=255)
                                          gAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGagcgcag  <  1:2034980/64‑1 (MQ=255)
                                          gAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGagcgcag  <  1:1014178/64‑1 (MQ=255)
                                          gAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGagcgcag  <  1:2537029/64‑1 (MQ=255)
                                          gAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGagcgcag  <  1:1086075/64‑1 (MQ=255)
                                          gAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGagcgcag  <  1:182222/64‑1 (MQ=255)
                                          gAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGagcgcag  <  1:2681593/64‑1 (MQ=255)
                                          gAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGagcgcag  <  1:1423970/64‑1 (MQ=255)
                                          gAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGagcgcag  <  1:2795752/64‑1 (MQ=255)
                                          gAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGagcgcag  <  1:2272597/64‑1 (MQ=255)
                                           aGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGagcgcag  <  1:2641987/63‑1 (MQ=255)
                                              |                                                           
TCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTTTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGAGCGCAG  >  W3110S.gb/2298522‑2298627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: