Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,307,310	C→T	V24V (GTC→GTT)	eco →	ecotin, a serine protease inhibitor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,307,310	0	C	T	100.0%	28.0 / NA	10	V24V (GTC→GTT)	eco	ecotin, a serine protease inhibitor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/2);  total (8/2)

CTGCAGTATTGTTTGCCGCTTTCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTCCAGCCACTGGAAAAAATCGCGCCTTATCCACAAGCTGA  >  W3110S.gb/2307255‑2307348                                                        |                                       cTGCAGTATTGTTTGCCGCTTTCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTTCAGCCACt                                >  1:1424839/1‑64 (MQ=255) cTGCAGTATTGTTTGCCGCTTTCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTTCAGCCACt                                >  1:1758874/1‑64 (MQ=255) cTGCAGTATTGTTTGCCGCTTTCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTTCAGCCACt                                >  1:1866619/1‑64 (MQ=255) cTGCAGTATTGTTTGCCGCTTTCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTTCAGCCACt                                >  1:2605723/1‑64 (MQ=255) cTGCAGTATTGTTTGCCGCTTTCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTTCAGCCACt                                >  1:530702/1‑64 (MQ=255)             ttGCCGCTTTCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTTCAGCCACTGGAAAAAATcgcg                   >  1:1433222/1‑65 (MQ=255)             ttGCCGCTTTCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTTCAGCCACTGGAAAAAATcgcg                   >  1:2047881/1‑65 (MQ=255)             ttGCCGCTTTCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTTCAGCCACTGGAAAAAATcgcg                   >  1:49958/1‑65 (MQ=255)                              aCTTCCGCCTGGGCGGCAGAAAGCGTTCAGCCACTGGAAAAAATCGCGCCTTATCCACAAGCTGa  <  1:2149383/65‑1 (MQ=255)                              aCTTCCGCCTGGGCGGCAGAAAGCGTTCAGCCACTGGAAAAAATCGCGCCTTATCCACAAGCTGa  <  1:766239/65‑1 (MQ=255)                                                        |                                       CTGCAGTATTGTTTGCCGCTTTCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTCCAGCCACTGGAAAAAATCGCGCCTTATCCACAAGCTGA  >  W3110S.gb/2307255‑2307348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑