Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,387,662	T→C	Y94H (TAT→CAT)	elaD →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,387,662	0	T	C	100.0%	106.0 / NA	29	Y94H (TAT→CAT)	elaD	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/13);  total (16/13)

ACCCTATCGATTATCCGACTTATCAAAAATGCTTGTTTAAAATTATATAATCTGGCACATATCGCAACCAACTCCCCCCTCAAATCACATGATTCAGATGATCTCC  >  W3110S.gb/2387617‑2387722                                              |                                                             aCCCTATCGATTATCCGACTTATCAAAAATGCTTGTTTAAAATTACATAATCTGGCACATATCGc                                           <  1:1098818/65‑1 (MQ=255) aCCCTATCGATTATCCGACTTATCAAAAATGCTTGTTTAAAATTACATAATCTGGCACATATCGc                                           <  1:696733/65‑1 (MQ=255) aCCCTATCGATTATCCGACTTATCAAAAATGCTTGTTTAAAATTACATAATCTGGCACATATCGc                                           <  1:2727622/65‑1 (MQ=255) aCCCTATCGATTATCCGACTTATCAAAAATGCTTGTTTAAAATTACATAATCTGGCACATATCGc                                           <  1:2504917/65‑1 (MQ=255)  cccTATCGATTATCCGACTTATCAAAAATGCTTGTTTAAAATTACATAATCTGGCACATATCGc                                           <  1:2215620/64‑1 (MQ=255)         gATTATCCGACTTATCAAAAATGCTTGTTTAAAATTACATAATCTGGCACATATCGcaaccaa                                     <  1:2387443/63‑1 (MQ=255)          aTTATCCGACTTATCAAAAATGCTTGTTTAAAATTACATAATCTGGCACATATCGc                                           <  1:2284954/56‑1 (MQ=255)          aTTATCCGACTTATCAAAAATGCTTGTTTAAAATTACATAATCTGGCACATATCGCAACCAACTc                                  >  1:1916028/1‑65 (MQ=255)           ttATCCGACTTATCAAAAATGCTTGTTTAAAATTACATAATCTGGCACATATCGCAACCAACTcc                                 >  1:1107543/1‑65 (MQ=255)                    ttATCAAAAATGCTTGTTTAAAATTACATAATCTGGCGCATATCGCAACCAACTCCCCCCTCaa                         >  1:707612/1‑64 (MQ=255)                    ttATCAAAAATGCTTGTTTAAAATTACATAATCTGGCACATATCGCAACCAACTCCCCCCTCaa                         >  1:2266655/1‑64 (MQ=255)                    ttATCAAAAATGCTTGTTTAAAATTACATAATCTGGCACATATCGCAACCAACTCCCCCCTCaa                         >  1:2687710/1‑64 (MQ=255)                            aaTGCTTGTTTAAAATTACATAATCTGGCACATATCGCAACCAACTCCCCCCTCAAATCACATg                 >  1:2073925/1‑64 (MQ=255)                            aaTGCTTGTTTAAAATTACATAATCTGGCACATATCGCAACCAACTCCCCCCTCAAATCACATGa                >  1:2087581/1‑65 (MQ=255)                            aaTGCTTGTTTAAAATTACATAATCTGGCACATATCGCAACCAACTCCCCCCTCAAATCACATGa                >  1:1504647/1‑65 (MQ=255)                               gCTTGTTTAAAATTACATAATCTGGCACATATCGCAACCAACTCCCCCCTC‑AATCACATGATTCa            <  1:712001/65‑1 (MQ=255)                                cTTGTTTAAAATTACATAATCTGGCACATATCGCAACCAACTCCCCCCTCAAATCATATGATTCa            <  1:724053/65‑1 (MQ=255)                                cTTGTTTAAAATTACATAATCTGGCACATATCGCAACCAACTCCCCCCTCAAATCACATGATTCa            <  1:857606/65‑1 (MQ=255)                                cTTGTTTAAAATTACATAATCTGGCACATATCGCAACCAACTCCCCCCTCAAATCACATGATTCa            <  1:131209/65‑1 (MQ=255)                                cTTGTTTAAAATTACATAATCTGGCACATATCGCAACCAACTCCCCCCTCAAATCACATGATTCa            <  1:1871262/65‑1 (MQ=255)                                cTTGTTTAAAATTACATAATCTGGCACATATCGCAACCAACTCCCCCCTCAAATCACATGATTCa            <  1:1096664/65‑1 (MQ=255)                                          aTTACATAATCTGGCACATATCGCAACCAACTCCCCCCTCAAATCACATGATTCAGATGATCTcc  >  1:1833237/1‑65 (MQ=255)                                          aTTACATAATCTGGCACATATCGCAACCAACTCCCCCCTCAAATCACATGATTCAGATGATCTcc  >  1:2615034/1‑65 (MQ=255)                                          aTTACATAATCTGGCACATATCGCAACCAACTCCCCCCTCAAATCACATGATTCAGATGATCTcc  >  1:351016/1‑65 (MQ=255)                                          aTTACATAATCTGGCACATATCGCAACCAACTCCCCCCTCAAATCACATGATTCAGATGATCTcc  >  1:1145697/1‑65 (MQ=255)                                          aTTACATAATCTGGCACATATCGCAACCAACTCCCCCCTCAAATCACATGATTCAGATGATCTcc  >  1:1890685/1‑65 (MQ=255)                                          aTTACATAATCTGGCACATATCGCAACCAACTCCCCCCTCAAATCACATGATTCAGATGATCTcc  >  1:724311/1‑65 (MQ=255)                                          aTTACATAATCTGGCACATATCGCAACCAACTCCCCCCTCAAATCACATGATTCAGATGATCTcc  >  1:2260907/1‑65 (MQ=255)                                          aTTACATAATCTGGCACATATCGCAACCAACTCCACCCTCAAATCACATGATTCAGATGATCTcc  >  1:2785197/1‑65 (MQ=255)                                              |                                                             ACCCTATCGATTATCCGACTTATCAAAAATGCTTGTTTAAAATTATATAATCTGGCACATATCGCAACCAACTCCCCCCTCAAATCACATGATTCAGATGATCTCC  >  W3110S.gb/2387617‑2387722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑