Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,426,403	G→A	G113R (GGG→AGG)	yfcG →	predicted glutathione S‑transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,426,403	0	G	A	100.0%	92.2 / NA	30	G113R (GGG→AGG)	yfcG	predicted glutathione S‑transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/1);  total (29/1)

TGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTAC  >  W3110S.gb/2426364‑2426450                                        |                                                tGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGc                        >  1:2143141/1‑65 (MQ=255) tGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGc                        >  1:548929/1‑65 (MQ=255) tGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGc                        >  1:805733/1‑65 (MQ=255) tGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGc                        >  1:819459/1‑65 (MQ=255) tGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGc                        >  1:93073/1‑65 (MQ=255) tGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGc                        >  1:2003207/1‑65 (MQ=255) tGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGc                        >  1:1458491/1‑65 (MQ=255) tGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGc                        >  1:1360738/1‑65 (MQ=255) tGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGc                        >  1:1319578/1‑65 (MQ=255)         cTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGcccacc                <  1:2328/65‑1 (MQ=255)                       gACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCtt    >  1:897510/1‑63 (MQ=255)                       gACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTa   >  1:1904080/1‑64 (MQ=255)                       gACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTAc  >  1:3031896/1‑65 (MQ=255)                       gACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTAc  >  1:903731/1‑65 (MQ=255)                       gACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTAc  >  1:896420/1‑65 (MQ=255)                       gACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTAc  >  1:886458/1‑65 (MQ=255)                       gACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTAc  >  1:941724/1‑65 (MQ=255)                       gACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTAc  >  1:574349/1‑65 (MQ=255)                       gACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTAc  >  1:984848/1‑65 (MQ=255)                       gACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTAc  >  1:374229/1‑65 (MQ=255)                       gACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTAc  >  1:3092361/1‑65 (MQ=255)                       gACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTAc  >  1:1127933/1‑65 (MQ=255)                       gACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTAc  >  1:3000197/1‑65 (MQ=255)                       gACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTAc  >  1:2668607/1‑65 (MQ=255)                       gACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTAc  >  1:2414861/1‑65 (MQ=255)                       gACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTAc  >  1:2314134/1‑65 (MQ=255)                       gACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTAc  >  1:2101358/1‑65 (MQ=255)                       gACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTAc  >  1:135305/1‑65 (MQ=255)                       gACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTAc  >  1:1268037/1‑65 (MQ=255)                       gACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCATTAc  >  1:41296/1‑65 (MQ=255)                                        |                                                TGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTAC  >  W3110S.gb/2426364‑2426450

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑