Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,440,837	C→T	A82A (GCG→GCA)	truA ←	pseudouridylate synthase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,440,837	0	C	T	100.0%	52.2 / NA	18	A82A (GCG→GCA)	truA	pseudouridylate synthase I
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/11);  total (7/11)

TTTTAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGCGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGT  >  W3110S.gb/2440779‑2440892                                                           |                                                        ttttAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCATGCcgcg                                                   >  1:1517118/1‑65 (MQ=255) ttttAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCATGCcgcg                                                   >  1:488785/1‑65 (MQ=255) ttttAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCATGCcgcg                                                   >  1:2797288/1‑65 (MQ=255) ttttAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCATGCcgcg                                                   >  1:2442245/1‑65 (MQ=255) ttttAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCATGCcgcg                                                   >  1:2377759/1‑65 (MQ=255) ttttAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCATGCcgcg                                                   >  1:23748/1‑65 (MQ=255) ttttAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCATGCcgcg                                                   >  1:1603300/1‑65 (MQ=255)                                                cccAAGGTCCATGCCGCGTCTTTGCGCAGCGCGGTTGTTTCGAAATGCACAACCTGCCCGGTAc     <  1:216784/64‑1 (MQ=255)                                                cccAAGGTCCATGCCGCGTCTTTGCGCAGCGCGGTTGTTTCGAAATGCACAACCTGCCCGGTAc     <  1:2145863/64‑1 (MQ=255)                                                cccAAGGTCCATGCCGCGTCTTTGCGCAGCGCGGTTGTTTCGAAATGCACAACCTGCCCGGTAc     <  1:2408650/64‑1 (MQ=255)                                                cccAAGGTCCATGCCGCGTCTTTGCGCAGCGCGGTTGTTTCGAAATGCACAACCTGCCCGGTAc     <  1:2662357/64‑1 (MQ=255)                                                cccAAGGTCCATGCCGCGTCTTTGCGCAGCGCGGTTGTTTCGAAATGCACAACCTGCCCGGTAc     <  1:2680237/64‑1 (MQ=255)                                                cccAAGGTCCATGCCGCGTCTTTGCGCAGCGCGGTTGTTTCGAAATGCACAACCTGCCCGGTAc     <  1:1590642/64‑1 (MQ=255)                                                cccAAGGTCCATGCCGCGTCTTTGCGCAGCGCGGTTGTTTCGAAATGCACAACCTGCCCGGTAc     <  1:3090217/64‑1 (MQ=255)                                                cccAAGGTCCATGCCGCGTCTTTGCGCAGCGCGGTTGTTTCGAAATGCACAACCTGCCCGGTAc     <  1:377905/64‑1 (MQ=255)                                                cccAAGGTCCATGCCGCGTCTTTGCGCAGCGCGGTTGTTTCGAAATGCACAACCTGCCCGGTAc     <  1:1374010/64‑1 (MQ=255)                                                cccAAGGTCCATGCCGCGTCTTTGCGCAGCGCGGTTGTTTCGAAATGCACAACCTGCCCGGTAc     <  1:824547/64‑1 (MQ=255)                                                  cAAGGTCCATGCCGCGTCTTTGCGCAGCGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCgt  <  1:522432/65‑1 (MQ=255)                                                           |                                                        TTTTAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGCGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGT  >  W3110S.gb/2440779‑2440892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑