Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,452,377	C→T	P181P (CCG→CCA)	aroC ←	chorismate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,452,377	0	C	T	100.0%	138.5 / NA	40	P181P (CCG→CCA)	aroC	chorismate synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (40/0);  total (40/0)

CGTCTAACGCGTCGATTTTGTCGGGGTCCGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAGTCTTT  >  W3110S.gb/2452349‑2452418                             |                                          cGTCTAACGCGTCGATTTTGTCTGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAg       >  1:592953/1‑65 (MQ=255) cGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTTGACCTGCGACCAg       >  1:166568/1‑65 (MQ=255) cGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAg       >  1:2999006/1‑65 (MQ=255) cGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAg       >  1:2213510/1‑65 (MQ=255) cGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAg       >  1:2357893/1‑65 (MQ=255) cGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAg       >  1:2487815/1‑65 (MQ=255) cGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAg       >  1:2630754/1‑65 (MQ=255) cGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAg       >  1:271175/1‑65 (MQ=255) cGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAg       >  1:2736976/1‑65 (MQ=255) cGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAg       >  1:293525/1‑65 (MQ=255) cGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAg       >  1:118180/1‑65 (MQ=255) cGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAg       >  1:414897/1‑65 (MQ=255) cGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAg       >  1:596174/1‑65 (MQ=255) cGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAg       >  1:650654/1‑65 (MQ=255) cGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAg       >  1:72946/1‑65 (MQ=255) cGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAg       >  1:737713/1‑65 (MQ=255) cGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAg       >  1:783402/1‑65 (MQ=255) cGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAg       >  1:2168570/1‑65 (MQ=255) cGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAg       >  1:1977104/1‑65 (MQ=255) cGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAg       >  1:1005042/1‑65 (MQ=255) cGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAg       >  1:1195604/1‑65 (MQ=255) cGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAg       >  1:1940500/1‑65 (MQ=255) cGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAg       >  1:1324732/1‑65 (MQ=255) cGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAg       >  1:1331007/1‑65 (MQ=255) cGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAg       >  1:1406730/1‑65 (MQ=255) cGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAg       >  1:1786620/1‑65 (MQ=255) cGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAg       >  1:1541993/1‑65 (MQ=255) cGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAg       >  1:1513217/1‑65 (MQ=255)                ttttGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGc             >  1:2992146/1‑44 (MQ=255)                ttttGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAGTCttt  >  1:819619/1‑55 (MQ=255)                ttttGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAGTCttt  >  1:660018/1‑55 (MQ=255)                ttttGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAGTCttt  >  1:1443295/1‑55 (MQ=255)                ttttGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAGTCttt  >  1:382080/1‑55 (MQ=255)                ttttGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAGTCttt  >  1:2811652/1‑55 (MQ=255)                ttttGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAGTCttt  >  1:1899500/1‑55 (MQ=255)                ttttGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAGTCttt  >  1:191824/1‑55 (MQ=255)                ttttGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAGTCttt  >  1:2704043/1‑55 (MQ=255)                ttttGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAGTCttt  >  1:2589835/1‑55 (MQ=255)                ttttGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAGTCttt  >  1:1957663/1‑55 (MQ=255)                ttttGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAGTCttt  >  1:2131533/1‑55 (MQ=255)                             |                                          CGTCTAACGCGTCGATTTTGTCGGGGTCCGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAGTCTTT  >  W3110S.gb/2452349‑2452418

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑