Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,469,991	T→C	S155G (AGC→GGC)	vacJ ←	predicted lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,469,991	0	T	C	100.0%	145.6 / NA	40	S155G (AGC→GGC)	vacJ	predicted lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/12);  total (28/12)

AGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACG  >  W3110S.gb/2469936‑2470031                                                        |                                         aGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCCACCGTAGaa                                 <  1:1456544/65‑1 (MQ=255)                            tCACCACCGTCATCACGCAGCGTGAAGCCACCGTAGaa                                 <  1:2458952/38‑1 (MQ=255)                                caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAAc                     >  1:830575/1‑46 (MQ=255)                                caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAAc                     >  1:486823/1‑46 (MQ=255)                                caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAAc                     >  1:2564911/1‑46 (MQ=255)                                caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAAc                     >  1:2406587/1‑46 (MQ=255)                                caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAAc                     >  1:2382997/1‑46 (MQ=255)                                caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAAc                     >  1:2124700/1‑46 (MQ=255)                                caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAAc                     >  1:2002247/1‑46 (MQ=255)                                caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAAc                     >  1:1817581/1‑46 (MQ=255)                                caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAAc                     >  1:1772498/1‑46 (MQ=255)                                caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAAc                     >  1:1505811/1‑46 (MQ=255)                                caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATACCCCAc   >  1:1119459/1‑64 (MQ=255)                                caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCAc   >  1:571944/1‑64 (MQ=255)                                caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCAc   >  1:329730/1‑64 (MQ=255)                                caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCAc   >  1:313918/1‑64 (MQ=255)                                caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCAc   >  1:2974004/1‑64 (MQ=255)                                caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCAc   >  1:296792/1‑64 (MQ=255)                                caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCAc   >  1:269145/1‑64 (MQ=255)                                caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCAc   >  1:686898/1‑64 (MQ=255)                                caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCAc   >  1:2624341/1‑64 (MQ=255)                                caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCAc   >  1:1290387/1‑64 (MQ=255)                                caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCAc   >  1:2503803/1‑64 (MQ=255)                                caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCAc   >  1:1290396/1‑64 (MQ=255)                                caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCAc   >  1:13521/1‑64 (MQ=255)                                caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCAc   >  1:2369054/1‑64 (MQ=255)                                caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCAc   >  1:2309048/1‑64 (MQ=255)                                caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCAc   >  1:2303244/1‑64 (MQ=255)                                caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCAc   >  1:1568989/1‑64 (MQ=255)                                caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCAc   >  1:705606/1‑64 (MQ=255)                                caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACg  <  1:651735/65‑1 (MQ=255)                                caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACg  <  1:789142/65‑1 (MQ=255)                                caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACg  <  1:635574/65‑1 (MQ=255)                                caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACg  <  1:1012671/65‑1 (MQ=255)                                caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACg  <  1:2868229/65‑1 (MQ=255)                                caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACg  <  1:2041241/65‑1 (MQ=255)                                caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACg  <  1:1876664/65‑1 (MQ=255)                                caccGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAAACCACg  <  1:2687886/65‑1 (MQ=255)                                 accGTCATCACGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACg  <  1:1755466/64‑1 (MQ=255)                                       atcaCGCAGCGTGAAGCCACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACg  <  1:516540/58‑1 (MQ=255)                                                        |                                         AGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACG  >  W3110S.gb/2469936‑2470031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑