Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,227,521	(G)5→4	coding (137/696 nt)	minC ←	cell division inhibitor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,227,517	0	G	.	100.0%	30.7 / NA	9	coding (141/696 nt)	minC	cell division inhibitor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (4/5);  total (4/5)

CCTTATGCATCGCTGACCAGTTTACCGGGTCTTCCAGTGCACTGACGTTGAGTACAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGCCTGATGGATAA  >  W3110S.gb/1227459‑1227578                                                           |                                                              ccTTATGCATCGCTGACCAGTTTACCGGGTCTTCCAGTGCACTGACGTTGAGTACAAC‑GGGGCATGTTTTa                                                  <  1:91912/71‑1 (MQ=255)            gCTGCCCAGTTTACCGGGTCTTCCAGTGCACTGACGTTGAGTACAAC‑GGGGCATGTTTTAAAAATGCgggg                                       >  1:3688959/1‑71 (MQ=255)                          cGGGTCTTCCAGTGCACTGACGTTGAGTACAAC‑GGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGAtttt                         <  1:1173447/71‑1 (MQ=255)                              tCTTCCAGTGCACTGACGTTGAGTACAAC‑GGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCt                     >  1:2806246/1‑71 (MQ=255)                                       gCACTGACGTTGAGTACAAC‑GGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGcc            >  1:3632106/1‑71 (MQ=255)                                          cTGACGTTGAGTACAAC‑GGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGAt                            <  1:3291414/52‑1 (MQ=255)                                          cTGACGTTGAGTACAAC‑GGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGAt                            <  1:4232238/52‑1 (MQ=255)                                            gACGTTGAGTACAAC‑GGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGCCTg          >  1:3981087/1‑68 (MQ=255)                                                  gAGTACAAC‑GGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGCCTGATGGATaa  <  1:121073/70‑1 (MQ=255)                                                           |                                                              CCTTATGCATCGCTGACCAGTTTACCGGGTCTTCCAGTGCACTGACGTTGAGTACAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGCCTGATGGATAA  >  W3110S.gb/1227459‑1227578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑