Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,530,233	Δ1 bp	coding (92/834 nt)	xapA ←	purine nucleoside phosphorylase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,530,230	0	A	.	100.0%	64.0 / NA	17	coding (95/834 nt)	xapA	purine nucleoside phosphorylase II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (6/11);  total (6/11)

AGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATAAAGGCCACTCGTGGCGTGAAATCAGGTTTATAAGTCTTGATAATATCTATGCAA  >  W3110S.gb/2530164‑2530289                                                                   |                                                            aGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTaaata                                                         <  1:4142466/71‑3 (MQ=255)                        aGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCT‑AAATAAAGGCCACTCGTGGCGTGAAATc                                 <  1:4529639/71‑1 (MQ=255)                          cGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCT‑AAATAAAGGCCACTCGTGGCGTGAAATCAg                               <  1:4614677/71‑1 (MQ=255)                            ttCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTaaat                                                          <  1:4503774/43‑2 (MQ=255)                              ctcAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCT‑AAATAAAGGCCACTCGTGGCGTGaaa                                   >  1:3903897/1‑63 (MQ=255)                              ctcAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCT‑AAATAAAGGCCACTCGTGGCGTGAAATCAGGtt                            >  1:1578230/1‑70 (MQ=255)                              ctcAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCT‑AAATAAAGGCCACTCGTGGCGTGAAATCAGGtt                            >  1:4367135/1‑70 (MQ=255)                              ctcAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCT‑AAATAAAGGCCACTCGTGGCGTGAAATCAGGtt                            >  1:1128633/1‑70 (MQ=255)                                  aTCTGATCGGCCAGCGCGCCGAGCCCGGAACCT‑AAATAAAGGCCACTCGTGGCGTGAAATCAGGTTTATaa                       <  1:4119456/71‑1 (MQ=255)                                  aTCTGATCGGCCAGCGCGCCGAGCCCGGAACCT‑AAATAAAGGCCACTCGTGGCGTGAAATCAGGTTTATaa                       <  1:3397444/71‑1 (MQ=255)                                          ggCCAGCGCGCCGAGCCCGGAACCT‑AAATAAAGGCCACTCGTGGCGTGAAATCAGGTTTATAAGTCTTGat               <  1:3588219/71‑1 (MQ=255)                                          ggCCAGCGCGCCGAGCCCGGAACCT‑AAATAAAGGCCACTCGTGGCGTGAAATCAGGTTTATAAGTCTTGat               <  1:228057/71‑1 (MQ=255)                                          ggCCAGCGCGCCGAGCCCGGAACCT‑AAATAAAGGCCACTCGTGGCGTGAAATCAGGTTTATAAGTCTTGat               <  1:1660492/71‑1 (MQ=255)                                                       aGCCCGGAACCT‑AAATAAAGGCCACTCGTGGCGTGAAATCAGGTTTATAAGTCTTGATAATATCTATGCaa  <  1:4176568/71‑1 (MQ=255)                                                       aGCCCGGAACCT‑AAATAAAGGCCACTCGTGGCGTGAAATCAGGTTTATAAGTCTTGATAATATCTATGCaa  <  1:43516/71‑1 (MQ=255)                                                       aGCCCGGAACCT‑AAATAAAGGCCACTCGTGGCGTGAAATCAGGTTTATAAGTCTTGATAATATCTATGCaa  <  1:4535747/71‑1 (MQ=255)                                                       aGCCCGGAACCT‑AAATAAAGGCCACTCGTGGCGTGAAATCAGGTTTATAAGTCTTGATAATATCTATGCa   >  1:2938118/1‑70 (MQ=255)                                                       aGCCCGGAACCT‑AAATAAAGGCCACTCGTGGCGTGAAATCAGGTTTATAAGTCTTGATAATATCTATGCa   >  1:2105219/1‑70 (MQ=255)                                                             gAACCT‑AAATAAAGGCCACTCGTGGCGTGAAATCAGGTTTATAAGTCTTGATAATATc         <  1:654368/58‑1 (MQ=255)                                                                   |                                                            AGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATAAAGGCCACTCGTGGCGTGAAATCAGGTTTATAAGTCTTGATAATATCTATGCAA  >  W3110S.gb/2530164‑2530289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑