BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,897,815	Δ1 bp	coding (260/1455 nt)	ygcU ←	predicted FAD containing dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,897,814	0	T	.	100.0%	88.8 / NA	23	coding (261/1455 nt)	ygcU	predicted FAD containing dehydrogenase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (8/15); total (8/15)

TTAATGATTTGATTCATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCA  >  W3110S.gb/2897746‑2897878

ttAATGATTTGATTCATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCCCCtcg                                                                >  1:1758986/1‑69 (MQ=255)

ttAATGATTTGATTCATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCtcg                                                                >  1:1980900/1‑69 (MQ=255)

ttAATGATTTGATTCATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCtcg                                                                >  1:2047619/1‑69 (MQ=255)

ttAATGATTTGATTCATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCtcg                                                                >  1:4013777/1‑69 (MQ=255)

ttAATGATTTGATTCATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCtcg                                                                >  1:3375942/1‑69 (MQ=255)

ttAATGATTTGATTCATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCtcg                                                                >  1:2522458/1‑69 (MQ=255)

ttAATGATTTGATTCATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCtcg                                                                >  1:3081680/1‑69 (MQ=255)

ttAATGATTTGATTCATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCACACCtc                                                                 <  1:4149559/70‑2 (MQ=255)

           attCATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACC‑TCGGTGGCGGAAg                                                     <  1:4389439/70‑1 (MQ=255)

           attCATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACC‑TCGGTGGCGGAAGc                                                    <  1:2165057/71‑1 (MQ=255)

            ttCATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACC‑TCGGTGGCGGAAGc                                                    <  1:4379310/70‑1 (MQ=255)

              cATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACC‑TCGGTGGCGGAAGCAc                                                  >  1:1383192/1‑70 (MQ=255)

              cATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACC‑TCGGTGGCGGAAGCAc                                                  >  1:1922215/1‑70 (MQ=255)

              cATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACC‑TCGGTGGCGGAAGCAc                                                  >  1:4061868/1‑70 (MQ=255)

               aTGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACC‑TCGGTGGCGGAAGCAcc                                                 <  1:617378/70‑1 (MQ=255)

               aTGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACC‑TCGGTGGCGGAAGCAcc                                                 <  1:4199993/70‑1 (MQ=255)

                tGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACC‑TCGGTGGCGGAAGCACCgg                                               <  1:1415361/71‑1 (MQ=255)

                  gCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACC‑TCGGTGGCGGAAGCACCGGTa                                             >  1:4513877/1‑71 (MQ=255)

                                caccacCGAGTTTTCTACAACAGTTTCCAGCCCACC‑TCGGTGGCGGAAGCACCGGTACGCGGCACACCGtt                               <  1:3897268/71‑1 (MQ=255)

                                caccacCGAGTTTTCTACAACAGTTTCCAGCCCACC‑TCGGTGGCGGAAGCACCGGTACGCGGCACACCGtt                               <  1:1388207/71‑1 (MQ=255)

                                            ttCTACAACAGTTTCCAGCCCACC‑TCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTgcg                    <  1:4045770/70‑1 (MQ=255)

                                                   acaGTTTCCAGCCCACC‑TCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCAt             >  1:1563337/1‑70 (MQ=255)

                                                     aGTTTCCAGCCCACC‑TCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATaa           >  1:2439392/1‑70 (MQ=255)

                                                      gTTTCCAGCCCACC‑TCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATaaaa         >  1:2756896/1‑71 (MQ=255)

                                                         tCCAGCCCACC‑TCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAAtt       >  1:2550941/1‑70 (MQ=255)

                                                          ccAGCCCACC‑TCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTc      <  1:2221437/70‑1 (MQ=255)

                                                          ccAGCCCACC‑TCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTc      <  1:2003016/70‑1 (MQ=255)

                                                           cAGCCCACC‑TCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAg    <  1:3683277/71‑1 (MQ=255)

                                                             gCCCACC‑TCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGca  <  1:1723879/71‑1 (MQ=255)

                                                             gCCCACC‑TCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGca  <  1:4663078/71‑1 (MQ=255)

                                                             gCCCACC‑TCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGca  <  1:1322672/71‑1 (MQ=255)

TTAATGATTTGATTCATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCA  >  W3110S.gb/2897746‑2897878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`