| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 547,835 | +G | pseudogene (255/261 nt) pseudogene (255/1259 nt) |
ylbE → ylbE → |
ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment ECK0512:JW0508+JW0507:b4572; hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 547,831 | 1 | . | G | 97.1% | 115.5 / ‑3.7 | 35 | pseudogene (251/261 nt) pseudogene (251/1259 nt) | ylbE ylbE | ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment ECK0512:JW0508+JW0507:b4572; hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (17/17); total (17/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
CGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑GG‑GGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACT > W3110S.gb/547766‑547894 | cgtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑gg‑ggg < 1:1160563/71‑2 (MQ=255)cgtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑gg‑gg > 1:4314815/1‑70 (MQ=255) gcgcATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGG‑GGAAGTg < 1:270615/71‑1 (MQ=255) tGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGG‑GGAAGTTAACtt > 1:1392289/1‑71 (MQ=255) tGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGG‑GGAAGTGAACtt < 1:3800851/71‑1 (MQ=255) tGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGG‑GGAAGTGAACtt < 1:2883445/71‑1 (MQ=255) tCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGG‑GGAAGTGAACTTCa > 1:1673505/1‑71 (MQ=255) tCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGG‑GGAAGTGAACTTCa > 1:3822408/1‑71 (MQ=255) tCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGG‑GGAAGTGAACTTCa > 1:2055127/1‑71 (MQ=255) tGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGG‑GGAAGTGAACTTCAtt > 1:4447320/1‑71 (MQ=255) tGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGG‑GGAAGTGAACTTCAtt > 1:3565885/1‑71 (MQ=255) ttCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGG‑GGAAGTGAACTTCa < 1:421750/67‑1 (MQ=255) cGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGG‑GGAAGTGAACTTCa > 1:3082856/1‑65 (MQ=255) cGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGG‑GGAAGTGAACTTCATTCCtt > 1:93507/1‑71 (MQ=255) cGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑gg‑ggga > 1:3871374/1‑52 (MQ=255) gTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGG‑GGAAGTGAACTTCATTCCTTGTCAc < 1:4411404/71‑1 (MQ=255) ttGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGG‑GGAAGTGaa > 1:3858753/1‑54 (MQ=39) cGAAAGATGATGCGCAGGCGCTGGCAATACTGGAGCAGGG‑GGAAGTGAACTTCATTCCTTGTCACCAtgtg < 1:758995/71‑1 (MQ=255) cGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGG‑GGAAGTGAACTTCATTCCTTGTCACCAtgtg < 1:4084755/71‑1 (MQ=255) gAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGG‑GGAAGTGAACTTCATTCCTTGTCACCAtgtg > 1:740638/1‑70 (MQ=255) aGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGG‑GGAAGTGAACTTCATTCCTTGTCACCATGTGAATg > 1:2257511/1‑71 (MQ=255) tGAAGCGCAGGCGCTGGCAATACTGGAGCAGGG‑GGAAGTGAACTTCATTCCTTGTc > 1:122802/1‑56 (MQ=255) gcAGGCGCTGGCAATACTGGAGCAGGG‑GGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGc > 1:525146/1‑71 (MQ=255) cgcTGGCAATACTGGAGCAGGG‑GGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATg > 1:1940080/1‑71 (MQ=255) gCAATACTGGAGCAGGG‑GGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCgg < 1:3425348/71‑1 (MQ=255) gCAATACTGGAGCAGGG‑GGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCgg < 1:1710255/71‑1 (MQ=255) aTACTGTAGCAGGG‑GGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATTGGCGGTAt < 1:443276/71‑1 (MQ=255) tACTGGAGCAGGG‑GGAAGTGAACTTCATTCCTTGTCACCAtgtg < 1:3040233/44‑1 (MQ=38) tACTGGAGCAGGG‑GGAAGTGAACTTCATTCCTTGTCACCATGTGaa < 1:3831744/46‑1 (MQ=255) tACTGGAGCAGGG‑GGAAGTGAACTTCATTCCTTGTCACCATGTGaa < 1:3899184/46‑1 (MQ=255) tACTGGAGCAGGG‑GGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTAtt < 1:2957286/71‑1 (MQ=255) tACTGGAGCAGGG‑GGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTAt > 1:225497/1‑70 (MQ=255) tACTGGAGCA‑GGAGGAAGTGAACTTCATTCCTTGTCACCAtgtg < 1:500059/44‑1 (MQ=255) aCTGGAGCAGGG‑GGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTa > 1:4005743/1‑71 (MQ=255) aCTGGAGCAGGG‑GGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTa > 1:960016/1‑71 (MQ=255) cTGGAGCAGGG‑GGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTAc < 1:1717010/71‑1 (MQ=255) cTGGAGCAGGG‑GGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTAc < 1:135276/71‑1 (MQ=255) gAGCAGGG‑GGAAGTGAACTTCATTCCTTGTCATCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACt < 1:604317/69‑1 (MQ=255) | CGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑GG‑GGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACT > W3110S.gb/547766‑547894 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |