Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	883,162	+C	intergenic (‑6/+52)	deoR ← / ← ybjG	DNA‑binding transcriptional repressor/undecaprenyl pyrophosphate phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	883,159	1	.	C	100.0%	150.8 / NA	45	intergenic (‑3/+55)	deoR/ybjG	DNA‑binding transcriptional repressor/undecaprenyl pyrophosphate phosphatase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (24/21);  total (24/21)

TGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTCCATAAT‑CCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCAC  >  W3110S.gb/883089‑883221                                                                        |                                                               tGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTCCATAAt                                                                 <  1:4233724/71‑1 (MQ=255)   tAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTCCATAAT‑cc                                                              >  1:395919/1‑71 (MQ=255)   tAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTCCATAAT‑cc                                                              >  1:852639/1‑71 (MQ=255)   tAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTCCATAAT‑cc                                                              >  1:4506850/1‑71 (MQ=255)   tAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTCCATAAT‑cc                                                              >  1:4550890/1‑71 (MQ=255)   tAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTCCATAAT‑c                                                               <  1:2718890/70‑1 (MQ=255)   tAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTCCATAAT‑c                                                               <  1:2382462/70‑1 (MQ=255)     aCTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTCCATAAT‑cccc                                                            <  1:1872891/71‑2 (MQ=255)     aCTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTCCATAAT‑ccc                                                             <  1:3449559/70‑1 (MQ=255)          tCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTCCATAAT‑c                                                               <  1:1518205/63‑1 (MQ=255)            gCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTCCATAAT‑ccc                                                             >  1:1035809/1‑63 (MQ=255)            gCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTCCATAAT‑ccc                                                             >  1:3850259/1‑63 (MQ=255)            gCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTCCATAAT‑cc                                                              >  1:4625693/1‑62 (MQ=255)               gCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTCCATAATCCCCTCTGaa                                                       >  1:3566367/1‑67 (MQ=255)               gCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTCCATAATCCCCTCTGaa                                                       >  1:3931931/1‑67 (MQ=255)               gCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTCCATAATCCCCTCTGaa                                                       >  1:4072796/1‑67 (MQ=255)               gCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTCCATAATCCCCTCTGaa                                                       >  1:2068863/1‑67 (MQ=255)               gCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTCCATAAT‑cccct                                                           >  1:2107053/1‑60 (MQ=255)                cGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTCCATAATCCCCTCTGAATAGtt                                                  <  1:3597207/71‑1 (MQ=255)                cGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTCCATAATCCCCTCTGAATAGt                                                   <  1:1254052/70‑1 (MQ=255)                cGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTCCATAATCCCCTCTGAATAGt                                                   <  1:622212/70‑1 (MQ=255)                  ttttAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTCCATAATCCCCTCTGAATAGt                                                   <  1:3294170/68‑1 (MQ=255)                  ttttAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTCCATAATCCCCTCTGAATAGt                                                   <  1:1231795/68‑1 (MQ=255)                  ttttAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTCCATAAT‑cccc                                                            <  1:2797597/58‑2 (MQ=255)                   tttAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTCCATAAT‑cccc                                                            <  1:2504433/57‑2 (MQ=255)                    ttAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTCCATAATCCCCTCTGAATAGTTAtt                                               >  1:4049957/1‑70 (MQ=255)                    ttAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTCCATAATCCCCTCTGAATAGTTAtt                                               >  1:1214693/1‑70 (MQ=255)                    ttAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTCCATAATCCCCTCTGAATAGTTAt                                                >  1:878443/1‑69 (MQ=255)                          cTTGCCGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTCCATAATCCCCTCTGAATAGTTATTGAAg                                           >  1:3650649/1‑68 (MQ=255)                          cTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTCCATAATCCCCTCTGAATAGTTATTGAAGc                                          >  1:3580710/1‑69 (MQ=255)                                gcagcTGCCCGATACGCTCTTCGCGACGTGTTTCCATAAT‑c                                                               <  1:1248103/41‑1 (MQ=255)                                   gcTGCCCGATACGCTCTTCGCGACGTGTTTCCATAATCCCCTCTGAATAGTTATTGAAGCGAGCCGCTCaa                               <  1:4255320/71‑1 (MQ=255)                                   gcTGCCCGATACGCTCTTCGCGACGTGTTTCCATAATCCCCTCTGAATAGTTATTGAAGCGAGCCGCTCaa                               <  1:3139875/71‑1 (MQ=255)                                   gcTGCCCGATACGCTCTTCGCGACGTGTTTCCATAATCCCCTCTGAATAGTTATTGAAGCGAGCCGCTCaa                               <  1:3462664/71‑1 (MQ=255)                                   gcTGCCCGATACGCTCTTCGCGACGTGTTTCCATAATCCCCTCTGAATAGTTATTGAAGCGAGCCGCTCaa                               <  1:4091735/71‑1 (MQ=255)                                      gCCCGATACGCTCTTCGCGACGTGTTTCCATAATCCCCTCTGAATAGtt                                                  >  1:3592287/1‑49 (MQ=255)                                      gCCCGATACGCTCTTCGCGACGTGTTTCCATAATCCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAAtac                            >  1:2769027/1‑71 (MQ=255)                                           aTACGCTCTTCGCGACGTGTTTCCATAATCCCCTCTGAATAGt                                                   <  1:33726/43‑1 (MQ=38)                                           aTACGCTCTTCGCGACGTGTTTCCATAATCCCCTCTGAATAGt                                                   <  1:125513/43‑1 (MQ=38)                                              cGCTCTTCGCGACGTGTTTCCATAATCCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACttt                    >  1:1159351/1‑71 (MQ=255)                                              cGCTCTTCGCGACGTGTTTCCATAATCCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACttt                    >  1:4524492/1‑71 (MQ=255)                                              cGCTCTTCGCGACGTGTTTCCATAATCCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACttt                    >  1:2865109/1‑71 (MQ=255)                                              cGCTCTTCGCGACGTGTTTCCATAATCCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACttt                    >  1:1935074/1‑71 (MQ=255)                                              cGCTCTTCGCGACGTGTTTCCATAATCCCCTATGAATAGTTATTGAAGCGAGCCGCTCAAta                             >  1:2496260/1‑62 (MQ=255)                                               gCTCTTCGCGACGTGTTTCCATGATCCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACtttt                   >  1:3094107/1‑71 (MQ=255)                                                   ttCGCGACGTGTTTCCATAATCCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAAtac                            <  1:3023019/58‑1 (MQ=255)                                                     cgcgACGTGTTTCCATAATCCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAg                >  1:3593735/1‑68 (MQ=255)                                                     cgcgACGTGTTTCCATAATCCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAg                <  1:1447704/68‑1 (MQ=255)                                                      gcgACGTGTTTCCATAATCCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCaga            <  1:684320/71‑1 (MQ=255)                                                      gcgACGTGTTTCCATAATCCCCTCTGAATAGTTATTGAAGCGAG‑‑GCTCAATACTACACTTTTTAGCagag           <  1:1542033/70‑1 (MQ=255)                                                           gtgtTTCCATAATCCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCagag           <  1:1258534/67‑1 (MQ=255)                                                            tgtTTCCATAATCCCCTCTGAATAGTTATTGAAGCGAGCCGCTc                                 <  1:2218939/44‑1 (MQ=38)                                                            tgtTTCCATAATCCCCTCTGAATAGTTATTGAAGCGAGCCGCTc                                 <  1:4300905/44‑1 (MQ=38)                                                              tttCCATAATCCCCTCTGAATAGTTATTGAAGCGATCCGCTCAATACTACACTTTTTAGCAGAGATCAGt     <  1:1274077/70‑1 (MQ=255)                                                              tttCCATAATCCCCTCTGAATAGTTATTGAAGCGAGCCGCTCaa                               >  1:1838986/1‑44 (MQ=38)                                                              tttCCATAATCCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGt     <  1:973950/70‑1 (MQ=255)                                                              tttCCATAATCCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGt     <  1:1335954/70‑1 (MQ=255)                                                              tttCCATAATCCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGt     >  1:3380921/1‑70 (MQ=255)                                                                tCCATAATCCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCa   >  1:4556729/1‑70 (MQ=255)                                                                tCCATAATCCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCa   >  1:3456209/1‑70 (MQ=255)                                                                tCCATAATCCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCAc  >  1:1733796/1‑71 (MQ=255)                                                                tCCATAATCCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACAATTTTTAGCAGAGATCAGTCa   >  1:4534791/1‑70 (MQ=255)                                                                        |                                                               TGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTCCATAAT‑CCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCAC  >  W3110S.gb/883089‑883221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑