Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,919,937	+C	coding (714/2634 nt)	yebT →	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,919,937	1	.	C	100.0%	117.1 / NA	34	V238V (GTG→GTC)	yebT	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (22/12);  total (22/12)

GAAAAAAGGTAGCCGTTTCTGGAACGTTTCCGGCGTTGATGCCAACGTCAGTATCAGTGGCGCGAAGGTG‑AAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCGATTGCCTTCGATTCACCAGAAGAGTCG  >  W3110S.gb/1919868‑1920000                                                                       |                                                                gAAAAAAGGTAGCCGTTTCTGGAACGTTTCCGGCGTTGATGCCAACGTCAGTATCAGTGGCGCGAAGGTg                                                                  >  1:1833339/1‑70 (MQ=255)  aaaaaaGGTAGCCGTTTCTGGAACGTTTCCGGCGTTGATGCCAACGTCAGTATCAGTGGCGCGAAGGTgca                                                                <  1:3992195/71‑3 (MQ=255)         gTAGCCGTTTCTGGAACGTTTCCGGCGTTGATGCCAACGTCAGTATCAGTGGCGCGAAGGTGCAAACTgg                                                          >  1:3672989/1‑70 (MQ=255)          tAGCCGTTTCTGGAACGTTTCCGGCGTTGATGCCAACGTCAGTATCAGTGGCGCGAAGGTGCAAACTGGa                                                         <  1:3349669/70‑1 (MQ=255)                  tCTGGAACGTTTCCGGCGTTGATGCCAACGTCAGTATCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTggc                                                >  1:2736078/1‑71 (MQ=255)                   cTGGAACGTTTCCGGCGTTGATGCCAACGTCAGTATCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTggcg                                               >  1:3630128/1‑71 (MQ=255)                      gAACGTTTCCGGCGTTGATGCCAACGTCAGTATCAGTGGCTCGAAGGTGCAAACTGGAAAGCCTggcgg                                              <  1:3549244/69‑1 (MQ=255)                      gAACGTTTCCGGCGTTGATGCCAACGTCAGTATCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTggcgg                                              <  1:2194595/69‑1 (MQ=255)                       aaCGTTTCCGGCGTTGATGCCAACGTCAGTATCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCAc                                           >  1:3897707/1‑71 (MQ=255)                        aCGTTTCCGGCGTTGATGCCAACGTCAGTATCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACt                                          <  1:2690449/71‑1 (MQ=255)                             tCCGGCGTTGATGCCAACGTCAGTATCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACTGGtt                                      <  1:3913105/70‑1 (MQ=255)                             tCCGGCGTTGATGCCAACGTCAGTATCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACTGGtt                                      >  1:1866286/1‑70 (MQ=255)                                    ttGATGCCAACGTCAGTATCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGc                              <  1:1906019/71‑1 (MQ=255)                                      gATGCCAACGTCAGTATCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCg                             <  1:4528767/70‑1 (MQ=255)                                      gATGCCAACGTCAGTATCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCg                             <  1:1670098/70‑1 (MQ=255)                                      gATGCCAACGTCAGTATCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCg                             <  1:3616655/70‑1 (MQ=255)                                      gATGCCAACGTCAGTATCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCGa                            >  1:3979501/1‑71 (MQ=255)                                      gATGCCAACGTCAGTATCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCGa                            >  1:23203/1‑71 (MQ=255)                                      gATGCCAACGTCAGTATCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCGa                            >  1:377846/1‑71 (MQ=255)                                      gATGCCAACGTCAGTATCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCGa                            >  1:1711228/1‑71 (MQ=255)                                          ccAACGTCAGTATCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCGATTGc                        >  1:3259306/1‑71 (MQ=255)                                                tCAGTATCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCg                             <  1:4570270/60‑1 (MQ=255)                                                tCAGTATCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCg                             <  1:4564895/60‑1 (MQ=255)                                                  aGTATGAGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCGATTGCCTTCGAtt                >  1:3922087/1‑71 (MQ=255)                                                  aGTATCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCGATTGCCTTCGAtt                >  1:4117896/1‑71 (MQ=255)                                                  aGTATCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCGATTGCCTTCGAtt                >  1:2368298/1‑71 (MQ=255)                                                     aTCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCa                                            >  1:2412330/1‑40 (MQ=37)                                                     aTCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCGATTGCCTTCGATTCAc             >  1:3477091/1‑71 (MQ=255)                                                      tCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCGATTGCCTTCGATTCAc             >  1:3324629/1‑70 (MQ=255)                                                      tCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCGATTGCCTTCGATTCAc             >  1:3304571/1‑70 (MQ=255)                                                        aGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCGATTGCCTTCGATTCAc             >  1:2557560/1‑68 (MQ=255)                                                        aGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCGATTGCCTTCGATTCAc             >  1:2305389/1‑68 (MQ=255)                                                           ggCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCGATTGCCTTCGATTCACCag          >  1:4099559/1‑68 (MQ=255)                                                            gcgcGAAGGTGCAAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCGATTGCCTTCGATTCACCagaag       <  1:3239047/70‑1 (MQ=255)                                                            gcgcGAAGGTGCAAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCGATTGCCTTCGATTCACCagaa        >  1:3527691/1‑69 (MQ=255)                                                                 aaGGTGCAAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCGATTGCCTTCGATTCACCAGAAGAGTCg  >  1:3707677/1‑70 (MQ=255)                                                                       |                                                                GAAAAAAGGTAGCCGTTTCTGGAACGTTTCCGGCGTTGATGCCAACGTCAGTATCAGTGGCGCGAAGGTG‑AAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCGATTGCCTTCGATTCACCAGAAGAGTCG  >  W3110S.gb/1919868‑1920000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑