New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb = 12093822 (0.030)35 (0.730) 22/96 0.2 90.3% coding (305/630 nt) ycfK hypothetical protein
?W3110S.gb = 1211179 6 (0.130)pseudogene (54/537 nt) stfE ECK1143:JW5172:b1157; e14 prophage region; predicted side tail fiber protein fragment

CTGTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCACTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/1209297‑1209382
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttggtttgggagaaggCTCTGCATTACCTGTTGGTGTCCCGGTTCCGTGGCCTTCAGCCACTCCGCCAACAGGCTGGCTGAAATGC  <  W3110S.gb/1211179‑1211110
                                                                                                                                                            
CTGTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCACTGTGCAAACGGCTCTCGAAAACCT                                                                                       <  1:950495/71‑1
     AAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCACTGTGCAAACGGCTCTCGAAAACCTTGGTT                                                                                  >  1:1662588/1‑71
         CGCAATAATCCGTTTGGCGATATCAAATCGGATGGCACTGTGCAAACGGCTCTCGAAAACCTTGGTTTGG                                                                               <  1:1397604/70‑1
             AGAATCCGTTTGGCGATATCAAATCGGATGGCACTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGA                                                                           <  1:2039315/70‑1
             AGAATCCGTTTGGCGATATCAAATCGGATGGCACTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAG                                                                            >  1:1706780/1‑69
               AATCCGTTTGGCGATATCAAATCGGATGGCACTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGG                                                                        <  1:3089671/71‑1
                ATCCGTTTGGCGATATCAAATCGGATGGCACTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGC                                                                       <  1:1490286/71‑1
                 TCCGTTTGGCGATATCAAATCGGATGGCACTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAA                                                                          <  1:4447981/67‑1
                 TCCGTTTGGCGATATCAAATCGGATGGCACTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAA                                                                          <  1:1558565/67‑1
                    GTTTGGCGATATCAAATCGGATGGCACTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGCTCTG                                                                   <  1:115361/71‑1
                    GTTTGGCGATATCAAATCGGATGGCACTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGCTCTG                                                                   <  1:4368682/71‑1
                    GTTTGGCGATATCAAATCGGATGGCACTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGCTCTG                                                                   <  1:1492877/71‑1
                    GTTTGGCGATATCAAATCGGATGGCACTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGCTCTG                                                                   <  1:3358722/71‑1
                    GTTTGGCGATATCAAATCGGATGGCACTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGCTCTG                                                                   <  1:4491342/71‑1
                     TTTGGCGATATCAAATCGGATGGCACTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGCTCTGC                                                                  <  1:4146708/71‑1
                     TTTGGCGATATCAAATCGGATGGCACTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGCTCTGC                                                                  <  1:1713642/71‑1
                     TTTGGCGATATCAAATCGGATGGCACTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGCTCTG                                                                   <  1:1424433/70‑1
                       TGGCGATATCAAATCGGATGGCACTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGCTCTGCAT                                                                >  1:3246343/1‑71
                         GCGATATCAAATCGGATGGCACTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGCTCTGCATTA                                                              <  1:758829/71‑1
                              ATCAAATCGGATGGCACTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGCTCTGCATTACCTGT                                                         >  1:4074679/1‑71
                                CAAATCGGATGGCACTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAG                                                                         >  1:4298922/1‑53
                                       GATGGCACTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGCTCTGCATTACCTGTTGGTGTCCC                                                <  1:729192/71‑1
                                         TGGCACTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGCTCTGCATTACCTGTTGG                                                      >  1:652601/1‑63
                                          GGCACTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGCTCTGCATTACCTGTT                                                        <  1:2177168/60‑1
                                          GGCACTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGCTCTGCATTACCTGTT                                                        <  1:372280/60‑1
                                             ACTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGCTCTGCATTACCTGTTGGTGTCCCGGTTCC                                          >  1:4281262/1‑71
                                              CTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGCTCTGCATTACCTGTTGGTGTCCCGGTTCCG                                         >  1:1482834/1‑71
                                                GTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGCTCTGCATTACCTGTTGGTGTCCCGGTTCCGTG                                       >  1:1860246/1‑71
                                                GTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGCTCTGCA                                                                 >  1:2397553/1‑45
                                                GTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGCTCTGCA                                                                 >  1:1816212/1‑45
                                                GTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGCTCTGCA                                                                 >  1:3400036/1‑45
                                                 TGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGCTCTGCATTACCTGTTGGTGTCCCGGTTCCGTGG                                      >  1:713598/1‑71
                                                 TGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGCTCTGCATTACCTGTTGGTGTCCCGGTTCCGTG                                       <  1:758458/70‑1
                                                   CAAACGGCTCTCTAAAACCTTGGTTTGGGAGAAGGCTCTGCATTACCTGTTGGTGTCCCGGTTCCGTGGCC                                    >  1:814841/1‑71
                                                   CAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGCTCTGCATTACCTGTTGGTGTCCCGGTTCCGTGGCC                                    <  1:994481/71‑1
                                                   CAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGCTCTGCATTACCTGTTGGTGTCCCGGTTCCGTGGCC                                    >  1:3644405/1‑71
                                                   CAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGCTCTGCATTACCTGTTGGTGTCCCGGTTCCGTGGCC                                    <  1:2212635/71‑1
                                                     AACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGCTCTGCATTACCTGTTGGTGTCCCGGTTCCGTGGCCT                                   <  1:1109105/70‑1
                                                         GCTCTCGAAAACCTTGGTTTGGGAGAAGGCTCTGCATTACCTGTTGGTGTCCCGGTTCCGTGGCCTTCAGC                              <  1:3467041/71‑1
                                                            CTCGAAAACCTTGGTTTGGGAGAAGGCTCTGCATTACCTGTTGGTGTCCCGGTTCCGTGGGCTTC                                 >  1:3944435/1‑65
                                                             TCGAAAACCTTGGTTTGGGAGAAGGCTCTGCATTACCTGTTGG                                                      <  1:1574303/43‑1
                                                               GAAAACCTTGGTTTGGGAGAAGGCTCTGCATTACCTGTTGGTGTCCCGGTTCCGTGGCCTTCAGCCAC                           <  1:3292201/68‑1
                                                               GAAAACCTTGGTTTGGGAGAAGGCTCTGCATTACCTGTTGGTGTCCCGGTTCCGTGGCCTTCAGCCAC                           <  1:633150/68‑1
                                                                    CCTTGGTTTGGGAGAAGGCTCTGCATTACCTGTTGGTGTCCCGGTTCCGTGGCCTTCAGCCACTCCGCCAA                   <  1:4040879/71‑1
                                                                                AGAAGGCTCTGCATTACCTGTTGGTGTCCCGGTTCCGTGGCCTTCAGCCACTCCGCCAACAGGCTGGCTGA       <  1:2304051/71‑1
                                                                                AGAAGGCTCTGCATTACCTGTTGGTGTCCCGGTTCCGTGGCCTTCAGCCACTCCGCCAACAGGCTGGCTGA       <  1:2235567/71‑1
                                                                                    GGCTCTGCATTACCTGTTGGTGTCCCGGTTCCGTGGCCTTCAGCCACTCCGCCAACAGGCTGGCTGAAAT    >  1:4480602/1‑70
                                                                                     GCTCTGCATTACCTGTTGGTGTCCCGGTTCCGTGGCCTTCAGCCACTCCGCCAACAGGCTGGCTGAAATGC  <  1:2968233/71‑1
                                                                                     GCTCTGCATTACCTGTTGGTGTCCCGGTTCCGTGGCCTTCAGCCACTCCGCCAACAGGCTGGCTGAAATGC  <  1:2800185/71‑1
                                                                                     GCTCTGCATTACCTGTTGGTGTCCCGGTTCCGTGGCCTTCAGCCACTCCGCCAACAGGCTGGCTGAAATGC  <  1:2794958/71‑1
                                                                                                                                                            
CTGTTAAGCCGCAAGAATCCGTTTGGCGATATCAAATCGGATGGCACTGTGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/1209297‑1209382
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttggtttgggagaaggCTCTGCATTACCTGTTGGTGTCCCGGTTCCGTGGCCTTCAGCCACTCCGCCAACAGGCTGGCTGAAATGC  <  W3110S.gb/1211179‑1211110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.