Predicted mutation
evidence seq id position mutation annotation gene description
MC JC W3110S.gb 3,261,134 Δ1,199 bp insH[tdcD] insH, [tdcD]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ W3110S.gb 3261134–3262331 3262332 2–1199 90 [0] [0] 90 insH–[tdcD] insH,[tdcD]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb = 32611330 (0.000)75 (1.250) 27/120 0.2 100% intergenic (‑59/‑69) tdcE/insH pyruvate formate‑lyase 4/2‑ketobutyrate formate‑lyase/IS5 element protein
?W3110S.gb 3262333 = 0 (0.000)coding (1183/1296 nt) tdcD propionate kinase/acetate kinase C, anaerobic

AATATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/3261060‑3261133
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGA  >  W3110S.gb/3262333‑3262401
                                                                                                                                               
AATATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGT                                                                          >  1:2525039/1‑71
AATATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGT                                                                          >  1:622339/1‑71
AATATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGT                                                                          >  1:4677025/1‑71
AATATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGT                                                                          >  1:45117/1‑71
    TCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAAC                                                                      >  1:4216864/1‑71
    TCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAAC                                                                      >  1:2964255/1‑71
    TCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTA                                                                        >  1:3178155/1‑69
    TCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGAGGGCGCGTTAAC                                                                      >  1:2433395/1‑71
        CCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGTCGCGTTAAC                                                                      <  1:3132015/67‑1
        CCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTG                                                                  >  1:2264130/1‑71
        CCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTT                                                                    <  1:2033854/69‑1
        CCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAAC                                                                      <  1:1086152/67‑1
        CCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAAC                                                                      <  1:2567211/67‑1
           TCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCC                                                                >  1:108719/1‑70
            CATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAAC                                                                      <  1:383236/63‑1
             ATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTATGCGGGCGCGTTAACTTTGCCTA                                                              <  1:3702417/70‑1
                AAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCTCGTTAACTTTGCCTAAATG                                                          <  1:3339590/71‑1
                AAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATG                                                          <  1:247927/71‑1
                AAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATG                                                          <  1:4589475/71‑1
                AAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATG                                                          <  1:4419979/71‑1
                AAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATG                                                          <  1:1844279/71‑1
                AAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATG                                                          <  1:2405587/71‑1
                AAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATG                                                          <  1:667957/71‑1
                AAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATG                                                          <  1:3037948/71‑1
                AAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATG                                                          <  1:3653023/71‑1
                AAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAAT                                                           >  1:2942790/1‑70
                AAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAAT                                                           >  1:2551842/1‑70
                AAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACCTTGCCTAAATG                                                          <  1:3007601/71‑1
                AAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGAGCGCGTTAACTTTGCCTAAAT                                                           >  1:12054/1‑70
                 AAAATAATCTCTCTACAATACTTCAACTAATTTATGCAAATTCTGCGTGCGCGTTAACTTTGCCTATATG                                                          <  1:1945163/70‑1
                 AAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATG                                                          <  1:2936093/70‑1
                     TAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGC                                                                 >  1:2532258/1‑59
                     TAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAAC                                                                      <  1:4270074/54‑1
                       ATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGG                                                     >  1:429908/1‑69
                           CTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCC                                                >  1:4076883/1‑70
                           CTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCC                                                >  1:74566/1‑70
                              TACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAG                                            >  1:1610830/1‑71
                                CAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGC                                           >  1:1512353/1‑70
                                CAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGC                                                    >  1:2868387/1‑61
                                 AATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAAC                                                                      <  1:4120192/42‑1
                                  ATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAA                                         >  1:2456077/1‑70
                                  ATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAA                                         >  1:2278625/1‑70
                                   TACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAAT                                        >  1:857799/1‑70
                                   TACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGA                                                         >  1:451966/1‑53
                                   TACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAA                                                            >  1:3032473/1‑50
                                    ACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAAGTTTGCCTAAATGAATGGCATCCAAAGCAATCA                                      >  1:1285237/1‑71
                                    ACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCA                                      >  1:1766833/1‑71
                                     CTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCAT                                     >  1:2246756/1‑71
                                     CTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCA                                      <  1:2290943/70‑1
                                     CTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATACAAAGCAATC                                       >  1:1185583/1‑69
                                     CTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAA                                                        >  1:671152/1‑52
                                     CTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAAC                                                                      >  1:3170680/1‑38
                                     CTTCAACTAAATTATGCAAATTATGCGGGCGCGTTAACTTTGCCTA                                                              <  1:241715/46‑1
                                       TCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAA                                                            >  1:627031/1‑46
                                         AACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTT                                  <  1:2524395/70‑1
                                         AACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAAAGAATGGCATCCAAAGCAATCATTTT                                  <  1:762482/70‑1
                                          ACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATG                                                          <  1:2318714/45‑1
                                           CTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTC                               <  1:2524884/71‑1
                                              AATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTC                            <  1:4131422/71‑1
                                              AATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCT                              <  1:3227171/69‑1
                                               ATTATCCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATG                                                          <  1:253420/40‑1
                                                TTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGT                          <  1:2560112/71‑1
                                                           CTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAAT                <  1:2015000/70‑1
                                                           CTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAAT                <  1:2560006/70‑1
                                                           CTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAAT                <  1:2346621/70‑1
                                                           CTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAAT                <  1:126269/70‑1
                                                           CTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAAT                <  1:3806933/70‑1
                                                           CTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAAT                <  1:903862/70‑1
                                                           CTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAAT                <  1:3603534/70‑1
                                                           CTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAAT                <  1:1698978/70‑1
                                                           CTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAAT                <  1:2545988/70‑1
                                                           CTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAAT                <  1:2874865/70‑1
                                                           CTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAAT                <  1:160772/70‑1
                                                           CTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAAT                <  1:2927224/70‑1
                                                           CTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAAT                <  1:2932796/70‑1
                                                           CTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAAT                <  1:1687229/70‑1
                                                              CGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAA              <  1:2720470/69‑1
                                                              CGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAA              <  1:1381500/69‑1
                                                              CGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAA              <  1:2714976/69‑1
                                                               GGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGG           <  1:541242/71‑1
                                                                      TTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGG                   <  1:1680948/56‑1
                                                                      TTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCT                              <  1:3017680/45‑1
                                                                      TTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCT                              <  1:384071/45‑1
                                                                        AACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGA  >  1:1319936/1‑71
                                                                        AACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGA  >  1:1401458/1‑71
                                                                        AACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGA  >  1:2607837/1‑71
                                                                        AACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGA  >  1:1599731/1‑71
                                                                        AACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATG   >  1:1407843/1‑70
                                                                         ACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGA  <  1:2245062/70‑1
                                                                         ACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGA  <  1:991601/70‑1
                                                                         ACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGA  <  1:648821/70‑1
                                                                         ACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGA  <  1:1011762/70‑1
                                                                         ACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGA  <  1:3154633/70‑1
                                                                         ACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGA  <  1:3459882/70‑1
                                                                         ACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGA  <  1:4409653/70‑1
                                                                                                                                               
AATATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/3261060‑3261133
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGA  >  W3110S.gb/3262333‑3262401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.