Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	9,442	T→C	L46S (TTA→TCA)	mog →	predicted molybdochelatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	9,442	0	T	C	100.0%	197.3 / NA	57	L46S (TTA→TCA)	mog	predicted molybdochelatase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/28);  total (29/28)

TCCCTGCGCTGGAAGAATGGCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTTAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGCTGGTGGATGAAATGAGTTGCCA  >  W3110S.gb/9379‑9508                                                                |                                                                   tCCCTGCGCTGGAAGAATGGCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTCAATcccc                                                             <  1:658069/71‑1 (MQ=255)   ccTGCGCTGGAAGAATGGCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTCAATcc                                                               >  1:125696/1‑67 (MQ=255)   ccTGCGCTGGAAGAATGGCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTCAATcc                                                               >  1:3378598/1‑67 (MQ=255)   ccTGCGCTGGAAGAATGGCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTCAATCCCCg                                                            >  1:3167187/1‑70 (MQ=255)   ccTGCGCTGGAAGAATGGCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTCAATCCCCGa                                                           >  1:278637/1‑71 (MQ=255)   ccTGCGCTGGAAGAATGGCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTCAATCCCCGa                                                           >  1:492761/1‑71 (MQ=255)   ccTGCGCTGGAAGAATGGCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTCAATCCCCGa                                                           >  1:616926/1‑71 (MQ=255)   ccTGCGCTGGAAGAATGGCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTCAATCCCCGa                                                           >  1:2920104/1‑71 (MQ=255)   ccTGCGCTGGAAGAATGGCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTCAATCCCCGa                                                           >  1:2534950/1‑71 (MQ=255)   ccTGCGCTGGAAGAATGGCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTCAATCCCCGa                                                           >  1:3552397/1‑71 (MQ=255)   ccTGCGCTGGAAGAATGGCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTCAATCCCCGa                                                           >  1:679785/1‑71 (MQ=255)   ccTGCGATGGAAGAATGGCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTCAATCCCCGa                                                           >  1:6831/1‑71 (MQ=255)       cgcTGGAAGAATGGCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTCAATCCCCGATGAg                                                       >  1:4052842/1‑71 (MQ=255)       cgcTGGAAGAATGGCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTCAATCCCCGATGAg                                                       >  1:597467/1‑71 (MQ=255)            gaagaaTGGCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTCAATCCCCGATGAGCAGGc                                                  >  1:3518488/1‑71 (MQ=255)             aagaaTGGCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTCAATCCCCGATGAGCAGGCg                                                 <  1:2961018/71‑1 (MQ=255)              agaaTGGCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTCAATCCCCGATGAGCAGGCg                                                 <  1:3145148/70‑1 (MQ=255)                aaTGGCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTCAATCCCCGATGAGCAGGCGat                                               >  1:2917023/1‑70 (MQ=255)                 aTGGCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTCAATCCCCGATGAGCAGGCGatca                                             <  1:2269722/71‑1 (MQ=255)                 aTGGCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTCAATCCCCGATGAGCAGGCGatc                                              <  1:3645282/70‑1 (MQ=255)                  tGGCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCGGCTCAATCCCCGATGAGCAGGCGatcat                                            >  1:3322466/1‑71 (MQ=255)                    gCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTCAATCCCCGATGAGCAGGCGatcatc                                           >  1:402898/1‑70 (MQ=255)                    gCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTCAATCCCCGATGAGCAGGCGatcat                                            >  1:870593/1‑69 (MQ=255)                        aCATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTCAATCCCCGATGAGCAGGCGat                                               <  1:1897203/62‑1 (MQ=255)                        aCATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTCAATCCCCGATGAGCAGGCGATCATCGAGCa                                      <  1:1361850/71‑1 (MQ=255)                        aCATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTCAATCCCCGATGAGCAGGCGATCATCGAGCa                                      <  1:790378/71‑1 (MQ=255)                          aTCGGCGCTAACCACGCCGTTTGAACTAGAAACCCGCTCAATCCCCGATGAGCAGGCGATCATCGAGCaaa                                    >  1:1629026/1‑71 (MQ=255)                           tCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTCAATCCCCGATGAGCAGGCGATCATCg                                          <  1:1691745/64‑1 (MQ=255)                             ggCGCTAACCACGCCGTTTGAACTGGAAACCCGCTCAATCCCCGATGAGCAGGCGATCATCGAGCAAACGt                                 <  1:2774235/71‑1 (MQ=255)                             ggCGCTAACCACGCCGTTTGAACTGGAAACCCGCTCAATCCCCGATGAGCAGGCGATCATCGAGCAAACGt                                 >  1:525164/1‑71 (MQ=255)                             ggCGCTAACCACGCCGTTTGAACTGGAAACCCGCTCAATCCCCGATGAGCAGGCGATCATCGAGCAAACGt                                 >  1:2539806/1‑71 (MQ=255)                             ggCGCTAACCACGCCGTTTGAACTGGAAACCCGCTCAATCCCCGATGAGCAGGCGATCATCGAGCAAACGt                                 >  1:1922533/1‑71 (MQ=255)                              gcgcTAACCACGCCGTTTGAACTGGAAACCCGCTCAATCCCCGATGAGCAGGCGATCATCGAGCAAACGtt                                <  1:3039693/71‑1 (MQ=255)                               cgcTAACCACGCCGTTTGAACTGGAAACCCGCTCAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTtg                               <  1:2668632/71‑1 (MQ=255)                               cgcTAACCACGCCGTTTGAACTGGAAACCCGCTCAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTtg                               <  1:3466580/71‑1 (MQ=255)                                gcTAACCACGCCGTTTGAACTGGAAACCCGCTCAATCCCCGATGAGCAGGCGATCATCGAGCAAACGt                                 <  1:765899/68‑1 (MQ=255)                                gcTAACCACGCCGTTTGAACTGGAAACCCGCTCAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTtg                               <  1:1866329/70‑1 (MQ=255)                                 cTAACCACGCCGTTTGAACTGGAAACCCGCTCAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTtgtg                             <  1:14056/71‑1 (MQ=255)                                  tAACCACGCCGTTTGAACTGGAAACCCGCTCAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTtg                               >  1:801660/1‑68 (MQ=255)                                    aCCACGCCGTTTGAACTGGAAACCCGCTCAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTtgtgt                            <  1:1583075/69‑1 (MQ=255)                                     ccACGCCGTTTGAACTGGAAACCCGCTCAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAg                         <  1:1831119/71‑1 (MQ=255)                                      cACGCCGTTTGAATTGGAAACCCGCTCAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGc                        >  1:2314201/1‑71 (MQ=255)                                       aCGCCGTTTGAACTGGAAACCCGCTCAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGCt                       <  1:3069045/71‑1 (MQ=255)                                        cGCCGTTTGAACTGGAAACCCGCTCAATCCCCGATGAGCAGGCGATCATCGAGCAAACGtt                                <  1:3233556/61‑1 (MQ=255)                                         gCCGTTTGAACTGGAAACCCGCTCAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGCtgg                     >  1:540038/1‑71 (MQ=255)                                               tGAACTGGAAACCCGCTCAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGCTGGTGGAt                >  1:972669/1‑70 (MQ=255)                                               tGAACTGGAAACCCGCTCAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGCTGGTGGAt                >  1:4324443/1‑70 (MQ=255)                                               tGAACTGGAAACCCGCTCAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGCTGGTGGAt                >  1:288300/1‑70 (MQ=255)                                               tGAACTGGAAACCCGCTCAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGCTGGTGGAt                >  1:163930/1‑70 (MQ=255)                                                 aaCTGGAAACCCGCTCAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGCTGGTGGATGa              <  1:50143/70‑1 (MQ=255)                                                 aaCTGGAAACCCGCTCAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGCTGGTGGATGa              <  1:4650805/70‑1 (MQ=255)                                                 aaCTGGAAACCCGCTCAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGCTGGTGGATGa              <  1:3507929/70‑1 (MQ=255)                                                  aCTGGAAACCCGCTCAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGCTGGTGGATGa              <  1:1924514/69‑1 (MQ=255)                                                      gAAACCCGCTCAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGCTGGTGGATGAAATg          <  1:2571299/69‑1 (MQ=255)                                                      gAAACCCGCTCAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGCTGGTGGATGAAATGa         <  1:1050649/70‑1 (MQ=255)                                                          cccGCTCAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGCTGGTGGATGAAATGAGTTGc    <  1:1240357/71‑1 (MQ=255)                                                            cGCTCAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGCTGGTGGATGAAATGAGTTGCCa  <  1:560213/71‑1 (MQ=255)                                                                |                                                                   TCCCTGCGCTGGAAGAATGGCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTTAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGCTGGTGGATGAAATGAGTTGCCA  >  W3110S.gb/9379‑9508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑