Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,927,326	G→A	V148M (GTG→ATG)	sdaC →	predicted serine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,927,326	0	G	A	100.0%	269.2 / NA	89	V148M (GTG→ATG)	sdaC	predicted serine transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (45/44);  total (45/44)

AGCTTCATGTCTCACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTATTCTGGT  >  W3110S.gb/2927260‑2927390                                                                   |                                                                 aGCTTCATGTCTCACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATggg                                                              <  1:3641215/71‑1 (MQ=255) aGCTTCATGTCTCACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATggg                                                              >  1:1320381/1‑71 (MQ=255) aGCTTCATGTCTCACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATggg                                                              >  1:421764/1‑71 (MQ=255) aGCTTCATGTCTCACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATggg                                                              <  1:3773067/71‑1 (MQ=255)     tCATGTCTCACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGCTCCTGATCATGGGTat                                                           >  1:2136658/1‑70 (MQ=255)      cATGTCTCACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTat                                                           <  1:3136382/69‑1 (MQ=255)      cATGTCTCACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTat                                                           <  1:2304568/69‑1 (MQ=255)      cATGTCTCACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTat                                                           <  1:909969/69‑1 (MQ=255)      cATGTCTCACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTat                                                           <  1:3584346/69‑1 (MQ=255)      cATGTCTCACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTat                                                           <  1:2044739/69‑1 (MQ=255)      cATGTCTCACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTat                                                           <  1:3759780/69‑1 (MQ=255)      cATGTCTCACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTat                                                           <  1:3768720/69‑1 (MQ=255)      cATGTCTCACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTat                                                           <  1:1649841/69‑1 (MQ=255)       aTGTCTCACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTatgat                                                        <  1:977977/71‑1 (MQ=255)       aTGTCTCACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTatgat                                                        <  1:3558829/71‑1 (MQ=255)       aTGTCTCACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTatgat                                                        <  1:4544598/71‑1 (MQ=255)          tctcACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGAc                                                     >  1:3612250/1‑71 (MQ=255)          tctcACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGAc                                                     >  1:2283456/1‑71 (MQ=255)          tctcACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGAc                                                     >  1:3333992/1‑71 (MQ=255)                      ggTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGc                                           <  1:2424470/69‑1 (MQ=255)                         aTGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATggg                                                              >  1:4406628/1‑47 (MQ=255)                         aTGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCgg                                      >  1:4664033/1‑71 (MQ=255)                         aTGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCg                                       >  1:292100/1‑70 (MQ=255)                         aTGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATAGTTCGCTTCg                                       >  1:2973739/1‑70 (MQ=255)                          tGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTatg                                                          <  1:2351886/50‑1 (MQ=255)                            aCGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCgg                                      >  1:2700976/1‑68 (MQ=255)                            aCGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGt                                     >  1:261711/1‑69 (MQ=255)                            aCGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGt                                     >  1:1481245/1‑69 (MQ=255)                            aCGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGt                                     >  1:3212508/1‑69 (MQ=255)                            aCGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGt                                     >  1:3218129/1‑69 (MQ=255)                            aCGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGt                                     >  1:227035/1‑69 (MQ=255)                            aCGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGt                                     >  1:1162460/1‑69 (MQ=255)                            aCGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGt                                     >  1:4418876/1‑69 (MQ=255)                            aCGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGt                                     >  1:3767843/1‑69 (MQ=255)                               ccACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCa                                <  1:1254277/71‑1 (MQ=255)                               ccACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCa                                <  1:2896868/71‑1 (MQ=255)                               ccACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCa                                <  1:411416/71‑1 (MQ=255)                                 aCCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGcg                                          >  1:2795407/1‑58 (MQ=255)                                 aCCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAg                                  >  1:1046397/1‑67 (MQ=255)                                 aCCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAg                                  >  1:806377/1‑67 (MQ=255)                                  ccgccgCGTGCGATTCTGTCGCTGATCCTGATCATGGGTat                                                           <  1:998093/41‑1 (MQ=255)                                   cgccgcGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAgatg                            <  1:1821913/71‑1 (MQ=255)                                   cgccgcGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAgatg                            <  1:1692390/71‑1 (MQ=255)                                   cgccgcGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAgatg                            <  1:61694/71‑1 (MQ=255)                                    gccgcGTGCGATTCTGTCGCTGATCCTGATCATGGGTatgat                                                        >  1:2548702/1‑42 (MQ=255)                                    gccgcGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGt                                               >  1:254602/1‑51 (MQ=255)                                    gccgcGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGt                                     >  1:249069/1‑61 (MQ=255)                                    gccgcGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAgatga                           >  1:2289994/1‑71 (MQ=255)                                    gccgcGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAgatga                           >  1:1765714/1‑71 (MQ=255)                                    gccgcGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAgatga                           >  1:3727322/1‑71 (MQ=255)                                      cgcgTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATc                         <  1:2317415/71‑1 (MQ=255)                                            cGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTa                     >  1:253619/1‑69 (MQ=255)                                            cGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTa                     >  1:2483822/1‑69 (MQ=255)                                            cGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTa                     >  1:4363367/1‑69 (MQ=255)                                            cGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTa                     >  1:1478643/1‑69 (MQ=255)                                              aTTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGc                 <  1:409051/71‑1 (MQ=255)                                              aTTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGc                 <  1:1014732/71‑1 (MQ=255)                                                tCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGa               >  1:1354941/1‑71 (MQ=255)                                                 cTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGAt              >  1:4618339/1‑71 (MQ=255)                                                 cTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGAt              >  1:4230947/1‑71 (MQ=255)                                                 cTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGAt              >  1:1013749/1‑71 (MQ=255)                                                 cTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGAt              >  1:2806241/1‑71 (MQ=255)                                                 cTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGAt              >  1:3004033/1‑71 (MQ=255)                                                    tCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGAt              <  1:4305136/68‑1 (MQ=255)                                                    tCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATg             <  1:2698535/69‑1 (MQ=255)                                                    tCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAg           <  1:778714/71‑1 (MQ=255)                                                    tCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAg           <  1:2395402/71‑1 (MQ=255)                                                    tCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAg           <  1:3074851/71‑1 (MQ=255)                                                      gCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTa         >  1:3561532/1‑71 (MQ=255)                                                      gCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTa         <  1:163415/71‑1 (MQ=255)                                                      gCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTa         <  1:3904015/71‑1 (MQ=255)                                                      gCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTa         >  1:4195336/1‑71 (MQ=255)                                                      gCTGATCCTAATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTa         <  1:3199814/71‑1 (MQ=255)                                                       ctgatcctgatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTa         <  1:874757/70‑1 (MQ=255)                                                            cctgatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTATTCTgg   >  1:185498/1‑71 (MQ=255)                                                            cctgatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTATTCTgg   >  1:1386219/1‑71 (MQ=255)                                                            cctgatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTATTCTgg   >  1:1786053/1‑71 (MQ=255)                                                             ctgatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTATTCTgg   <  1:228895/70‑1 (MQ=255)                                                             ctgatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTATTCTgg   <  1:1290956/70‑1 (MQ=255)                                                             ctgatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTATTCTgg   <  1:3406199/70‑1 (MQ=255)                                                             ctgatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTATTCTgg   <  1:1241852/70‑1 (MQ=255)                                                             ctgatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTATTCTgg   <  1:2725269/70‑1 (MQ=255)                                                             ctgatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTATTCTgg   <  1:1236488/70‑1 (MQ=255)                                                             ctgatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTATTCTgg   <  1:2131607/70‑1 (MQ=255)                                                             ctgatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTATTCTgg   <  1:2550587/70‑1 (MQ=255)                                                             ctgatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTATTCTGGt  <  1:141288/71‑1 (MQ=255)                                                             ctgatcATGGGTATGATGACAATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTATTCTgg   <  1:2118263/70‑1 (MQ=255)                                                                atcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAga                              >  1:2451629/1‑40 (MQ=255)                                                                atcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAga                              >  1:1048831/1‑40 (MQ=255)                                                                   |                                                                 AGCTTCATGTCTCACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTATTCTGGT  >  W3110S.gb/2927260‑2927390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑