| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 2,964,217 | G→A | N159N (AAC→AAT) | lgt ← | phosphatidylglycerol‑prolipoprotein diacylglyceryl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,964,217 | 0 | G | A | 97.4% | 107.2 / ‑3.8 | 39 | N159N (AAC→AAT) | lgt | phosphatidylglycerol‑prolipoprotein diacylglyceryl transferase |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (27/11); total (28/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
GCGGGTTGGTTTGCAGCAGCAAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACC > W3110S.gb/2964150‑2964282 | gCGGGTTGGTTTGCAGCAGCAAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAATTc > 1:2982738/1‑71 (MQ=255)gCGGGTTGGTTTGCAGCAGCAAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAATTc > 1:2690780/1‑71 (MQ=255)gCGGGTTGGTTTGCAGCAGCAAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAATTc > 1:2226612/1‑71 (MQ=255)gCGGGTTGGTTTGCAGCAGCAAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAATTc > 1:1550414/1‑71 (MQ=255)gCGGGTTGGTTTGCAGCAGCAAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAATTc > 1:4279344/1‑71 (MQ=255) ggttTGCAGCAGCAAAATATCTTCTGTAAGGGAGCCAGGGAACAGCATGGCAAACGGGAAATTCGGGTCaa > 1:1760339/1‑71 (MQ=255) tGCAGCAGCAAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAATTCggg > 1:760052/1‑63 (MQ=255) tGCAGCAGCAAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAATTCGGGTCAAcgcg > 1:4666657/1‑71 (MQ=255) tcttctGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGt > 1:2975623/1‑49 (MQ=255) tcttctGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAATTCGGGTCAACGCGGCCCCACAATTCAcc > 1:1260263/1‑71 (MQ=255) cGGGAGCCAGGGAACAGCATGGCAAACGGGAAATTCGGGTCAACGCGGCCCCACAATTCACCGTtaataa < 1:4147388/70‑1 (MQ=255) cGGGAGCCAGGGAACAGCATGGCAAACGGGAAATTCGGGTCAACGCGGCCCCACAATTCACCGTTAATaaa < 1:1369080/71‑1 (MQ=255) cGGGAGCCAGGGAACAGCATGGCAAACGGGAAATTCGGGTCAACGCGGCCCCACAATTCACCGTTAATaaa < 1:3612492/71‑1 (MQ=255) aGGGAACAGCATGGCAAACGGGAAATTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCa < 1:262259/71‑1 (MQ=255) aaCAGCATGGCAAACGGGAAATTCGGGTCAACGCgg > 1:2757533/1‑36 (MQ=255) aaCAGCATGGCAAACGGGAAATTCGGGTCAACGCGGcc > 1:3365697/1‑38 (MQ=255) aaCAGCATGGCAAACGGGAAATTCGGGTCAACGCGGCCCCACAATTCACCg > 1:1285031/1‑51 (MQ=255) aaCAGCATGGCAAACGGGAAATTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGa > 1:3185562/1‑69 (MQ=255) aaCAGCATGGCAAACGGGAAATTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACg > 1:3523138/1‑71 (MQ=255) aaCAGCATGGCAAACGGGAAATTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACg > 1:792913/1‑71 (MQ=255) aaCAGCATGGCAAACGGGAAATTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACg > 1:1606530/1‑71 (MQ=255) aCAGCATGGCAAACGGGAAATTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGt > 1:3169880/1‑60 (MQ=255) aCAGCATGGCAAACGGGAAATTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGcc < 1:4222092/64‑1 (MQ=255) aGCATGGCAAACGGGAAATTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCa < 1:3386803/64‑1 (MQ=255) gCATGGCAAACGGGAAATTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCg > 1:1404143/1‑71 (MQ=255) cATGGCAAACGGGAAATTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGa > 1:4662362/1‑64 (MQ=255) cATGGCAAACGGGAAATTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGa > 1:429802/1‑64 (MQ=255) cATGGCAAACGGGAAATTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGa > 1:424214/1‑64 (MQ=255) cATGGCAAACGGGAAATTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCgg > 1:4157235/1‑71 (MQ=255) tGGCAAACGGGAAATTCGGGTCAACGCGGCCCCACAATTc < 1:305422/40‑1 (MQ=255) tGGCAAACGGGAAATTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCa < 1:373929/60‑1 (MQ=255) ggCAAACGGGAAATTCGGGTCAACGCGGCCCCACAATTCACCTTTAATAAAGTTGCCCAGACGCCCGGc < 1:639977/69‑1 (MQ=255) gCAAACGGGAAATTCGGGTCAACGCGGCCCCACAATTCAcc > 1:1341822/1‑41 (MQ=255) gCAAACGGGAAATTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCAcc < 1:2627782/71‑1 (MQ=255) gCAAACGGGAAATTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCAcc < 1:3756733/71‑1 (MQ=255) gCAAACGGGAAATTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCAc > 1:2907156/1‑70 (MQ=255) gCAAACGGGAAATTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCAc > 1:3048988/1‑70 (MQ=255) cGGGAAATTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGAc > 1:1623471/1‑71 (MQ=255) ggAAATTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGAcc > 1:2731304/1‑70 (MQ=255) | GCGGGTTGGTTTGCAGCAGCAAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACC > W3110S.gb/2964150‑2964282 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |