Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,971,531	G→T	L330I (CTA→ATA)	ygeD ←	predicted inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,971,531	0	G	T	100.0%	64.2 / NA	24	L330I (CTA→ATA)	ygeD	predicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (4/20);  total (4/20)

TCGCCAAGGTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTAGCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCATCACGCCAATCAGCATCAACAAGGCA  >  W3110S.gb/2971467‑2971592                                                                 |                                                              tCGCCAAGGTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTATCAACg                                                          <  1:2067181/70‑1 (MQ=255)  cGCCAAGGTTTTGTACTGCAATCGCATTCCCCGCGCCGACGCTTTTTTTACCCCGCTCCTGTATCAACGc                                                         <  1:2306240/70‑1 (MQ=255)  cGCCAAGGTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTAGCCCGCTCCTGTATCAACGc                                                         <  1:911725/70‑1 (MQ=255)   gCCAAGGTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTATCAACGCa                                                        <  1:1960022/70‑1 (MQ=255)   gCCAAGGTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTATCAACGCa                                                        <  1:1631286/70‑1 (MQ=255)          ttttGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTATCAACGCATTGAGCgg                                                <  1:1149325/71‑1 (MQ=255)                   gCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTATCAACGCATTGAGCGGAACGACaa                                        >  1:2764198/1‑70 (MQ=255)                    cAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTATCAACGCATTGAGCGGAACGACaaaa                                      <  1:2331996/71‑1 (MQ=255)                     aaTCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTATCAACGCATTGAGCGGAACGACaaaaa                                     <  1:119159/71‑1 (MQ=255)                      aTCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTATCAACGCATTGAGCGGAACGACaaaaaa                                    <  1:1457095/71‑1 (MQ=255)                      aTCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTATCAACGCATTGAGCGGAACGACaaaaaa                                    <  1:2741971/71‑1 (MQ=255)                       tCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTATCAACg                                                          <  1:2769987/48‑1 (MQ=255)                            ttCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTATCaa                                                            >  1:1242008/1‑41 (MQ=255)                            ttCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTATCAACGCATTGAGCGGAACGACAAAAAAGccccc                              <  1:1536824/71‑1 (MQ=255)                               cccgccccgACGCTTTTTTTACCCCGCTCCTGTATCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCAt                           <  1:900301/71‑1 (MQ=255)                                           cTTTTTTTACCCCGCTCCTGTATCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCATCACGCCAATCAg               <  1:2157892/71‑1 (MQ=255)                                                      ccccTCCTGTATCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCATCACGCCAATCAGCATCAAAAAgg    <  1:4286269/68‑1 (MQ=255)                                                      ccGCTCCTGTATCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCATCACGCCAATCAGCATCAACAAgg    <  1:341192/71‑1 (MQ=255)                                                      ccGCTCCTGTATCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCATCACGCCAATCAGCATCAACAAgg    <  1:4397059/71‑1 (MQ=255)                                                      ccGCTCCTGTATCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCATCACGCCAATCAGCATCAACAAgg    <  1:1081529/71‑1 (MQ=255)                                                       cGCTCCTGTATCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCATCACGCCAATCAGCATCAACAAgg    <  1:1072290/70‑1 (MQ=255)                                                       cGCTCCTGTATCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCATCACGCCAATCAGCATCAACAAgg    >  1:4501770/1‑70 (MQ=255)                                                        gCTCCTGTATCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCATCACGCCAATCAGCATCAACAAGGCa  <  1:3168195/71‑1 (MQ=255)                                                           ccTGTATCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCATCAc                        >  1:3721801/1‑46 (MQ=255)                                                                 |                                                              TCGCCAAGGTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTAGCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCATCACGCCAATCAGCATCAACAAGGCA  >  W3110S.gb/2971467‑2971592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑