Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,065,306	A→G	V175A (GTT→GCT)	ygfI ←	predicted DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,065,306	0	A	G	100.0%	121.6 / NA	36	V175A (GTT→GCT)	ygfI	predicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/26);  total (10/26)

ATTTGTAGCCAGGGCAGCTCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCG  >  W3110S.gb/3065243‑3065370                                                                |                                                                 aTTTGTAGCCAGGGCAGCTCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGagagaagcgagaaa                                                           <  1:1626396/71‑1 (MQ=255)  tttGTAGCCAGGGCAGCTCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATTACGGCGAGAAGCGAGAAAg                                                          >  1:2249195/1‑71 (MQ=255)             ggCAGCTCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGAGAGAAAGCCTAATCGAAg                                               >  1:4204276/1‑71 (MQ=255)              gCAGCTCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGc                                              <  1:2134049/71‑1 (MQ=255)               cAGCTCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCg                                             >  1:261175/1‑71 (MQ=255)                   tCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCggtgg                                         >  1:1389521/1‑71 (MQ=255)                     cTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCggtggt                                        <  1:1873297/70‑1 (MQ=255)                     cTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCggtgg                                         >  1:2278765/1‑69 (MQ=255)                     cTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCggtgg                                         <  1:4487511/69‑1 (MQ=255)                     cTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCggtgg                                         <  1:2221238/69‑1 (MQ=255)                     cTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCggtgg                                         <  1:1939570/69‑1 (MQ=255)                     cTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTa                                       <  1:4392840/71‑1 (MQ=255)                     cTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTa                                       <  1:4476787/71‑1 (MQ=255)                       gAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCggtggt                                        <  1:1131748/68‑1 (MQ=255)                        aaGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGtt                                    <  1:4069558/71‑1 (MQ=255)                        aaGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGtt                                    <  1:773344/71‑1 (MQ=255)                        aaGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGtt                                    <  1:4495767/71‑1 (MQ=255)                         agcagcTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTc                                   <  1:2615776/71‑1 (MQ=255)                         agcagcTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTc                                   <  1:2118579/71‑1 (MQ=255)                            agcTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACt                                <  1:1777617/71‑1 (MQ=255)                                 ttgctcttgTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGAt                             <  1:1767966/69‑1 (MQ=255)                                 ttgctcttgTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAg                           >  1:2363391/1‑71 (MQ=255)                                 ttgctcttgTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAg                           >  1:1898350/1‑71 (MQ=255)                                    ctcttgTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCggt                                           <  1:3118941/52‑1 (MQ=255)                                    ctcttgTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCggt                                           <  1:1625261/52‑1 (MQ=255)                                    ctcttgTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCggt                                           <  1:584237/52‑1 (MQ=255)                                    ctcttgTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCggt                                           <  1:122042/52‑1 (MQ=255)                                            gCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTcc                <  1:4457021/71‑1 (MQ=255)                                            gCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTc                 >  1:4664388/1‑70 (MQ=255)                                               ctTCATAACGGAGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGt             >  1:3819639/1‑71 (MQ=255)                                                ttCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGtt                                    <  1:2047925/47‑1 (MQ=255)                                                  cATAACGGCGAGAAGCTAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGt             <  1:685565/68‑1 (MQ=255)                                                  cATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGtt            <  1:235474/69‑1 (MQ=255)                                                    tAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGa                              <  1:573811/49‑1 (MQ=255)                                                    tAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCt         >  1:3367787/1‑70 (MQ=255)                                                    tAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTg        <  1:4118691/71‑1 (MQ=255)                                                    tAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTg        <  1:4677175/71‑1 (MQ=255)                                                          cgagaagcgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCg  <  1:2286372/71‑1 (MQ=255)                                                                |                                                                 ATTTGTAGCCAGGGCAGCTCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCG  >  W3110S.gb/3065243‑3065370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑