Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,101,264	G→A	A82V (GCT→GTT)	yggH ←	tRNA (m7G46) methyltransferase, SAM‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,101,264	0	G	A	100.0%	108.3 / NA	39	A82V (GCT→GTT)	yggH	tRNA (m7G46) methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/19);  total (20/19)

AGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCAATCTCAAGCGTCACCGGCGCTTCA  >  W3110S.gb/3101198‑3101323                                                                   |                                                            aGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAACCAt                                                         <  1:4550930/71‑1 (MQ=255)     acgcacCAACGCCCGGTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAACCATTGc                                                      >  1:678885/1‑70 (MQ=255)        cacCAAAGCCCGGTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAACCATTGcc                                                     >  1:598039/1‑68 (MQ=255)           cAACGCCCGGTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAACCATTGCCACCAGc                                               <  1:122535/71‑1 (MQ=255)           cAACGCCCGGTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAACCATTGCCACCAGc                                               <  1:4575279/71‑1 (MQ=255)           cAACGCCCGGTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAACCATTGCCACCAGc                                               <  1:2456607/71‑1 (MQ=255)           cAACGCCCGGTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAACCATTGCCACCAGc                                               <  1:2451139/71‑1 (MQ=255)                   ggTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAACCATTGCCACCAGCGACGcccc                                       <  1:2485965/71‑1 (MQ=255)                   ggTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAACCATTGCCACCAGCGACGcccc                                       <  1:2777180/71‑1 (MQ=255)                   ggTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAACCATTGCCACCAGCGACGcccc                                       <  1:36045/71‑1 (MQ=255)                   ggTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAACCATTGCCACCAGCGACGcccc                                       <  1:796831/71‑1 (MQ=255)                      gAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAACCATTGCCACCAGCGACGCCCCCAt                                    >  1:3552478/1‑71 (MQ=255)                         tGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAACCATTGCCACCAGCGACGCCCCCATGc                                  >  1:1321308/1‑70 (MQ=255)                         tGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAACCATTGCCACCAGCGACGCCCCCATGc                                  >  1:761615/1‑70 (MQ=255)                           cACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAACCATTGCCACCAGCGACGCCCCCATGCCaa                               <  1:2382110/71‑1 (MQ=255)                             cTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAACCATTGCCACCAGCGACGcccc                                       <  1:4504944/61‑1 (MQ=255)                             cTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAACCATTGCCACCAGCGACGcccc                                       <  1:3024518/61‑1 (MQ=255)                               tCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAACCATTGCCACCAGCGACGCCCCCATGCCaaaa                             >  1:1078092/1‑69 (MQ=255)                               tCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAACCATTGCCACCAGCGACGCCCCCATGCCAAAAc                            >  1:2490774/1‑70 (MQ=255)                               tCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAACCATTGCCACCAGCGACGCCCCCATGCCAAAAc                            >  1:1808674/1‑70 (MQ=255)                               tCAATGCCGAGGAAGTCCTGCTAAGGGCGATCTTTAACCATTGCCACCAGCGACGCCCCCATGCCAAAAc                            >  1:744936/1‑70 (MQ=255)                                cAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAACCa                                                          >  1:2170924/1‑39 (MQ=255)                                cAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAACCATTGCCACCAGCGACGCCCCCa                                     >  1:4432640/1‑60 (MQ=255)                                cAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAACCATTGCCACCAGCGACGCCCCCATGCCAAAAcc                           <  1:16870/70‑1 (MQ=255)                                cAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAACCATTGCCACCAGCGACGCCCCCATGCCAAAAcc                           <  1:562702/70‑1 (MQ=255)                                  aTGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAACCATTGCCACCAGCGACGcccc                                       <  1:377444/56‑1 (MQ=255)                                    gCCGAGGAAGTCCTGCTCAGGGCGATCTTTAACCATTGCCACCAGCGa                                             >  1:691893/1‑48 (MQ=255)                                    gCCGAGGAAGTCCTGCTCAGGGCGATCTTTAACCATTGCCACCAGCGACGCCCCCa                                     >  1:4271221/1‑56 (MQ=255)                                     ccGAGGAAGTCCTGCTCAGGGCGATCTTTAACCATTGCCACCAGCGACGCCCCCATGCCAAAACCAAtct                      >  1:2078359/1‑70 (MQ=255)                                          gAAGTCCTGCTCAGGGCGATCTTTAACCATTGCCACCAGCGACGCCCCCa                                     <  1:3360182/50‑1 (MQ=255)                                            aGTCCTGCTCAGGGCGATCTTTAACCATTGCCACCAGCGACGCCCCCATGCCAAAACCAATCTCAAGCGTc              <  1:1614085/71‑1 (MQ=255)                                               ccTGCTCAGGGCGATCTTTAACCATTGCCACCAGCGACGCCCCCa                                     >  1:3615499/1‑45 (MQ=255)                                               ccTGCTCAGGGCGATCTTTAACCATTGCCACCAGCGACGCCCCCa                                     >  1:66732/1‑45 (MQ=255)                                               ccTGCTCAGGGCGATCTTTAACCATTGCCACCAGCGACGCCCCCATGCCAAAACCAATCTCAAGCGTCAcc           >  1:3858380/1‑71 (MQ=255)                                                cTGCTCAGGGCGATCTTTAACCATTGCCACCAGCGACGCCCCCATGCCAAAACCAATCTCaa                   >  1:3659669/1‑62 (MQ=255)                                                cTGCTCAGGGCGATCTTTAACCATTGCCACCAGCGACGCCCCCATGCCAAAACCAATCTCAAGCGTCACCg          >  1:531448/1‑71 (MQ=255)                                                  gCTCAGGGCGATCTTTAACCATTGCCACCAGCGACGccc                                        <  1:91386/39‑1 (MQ=255)                                                         gCGATCTTTAACCATTGCCACCAGCGACGCCCCCATGCCAAAACCAATCTCAAGCGTCACCGGCGCTTCa  >  1:3576172/1‑70 (MQ=255)                                                            aTCTTTAACCATTGCCACCAGCGACGCCCCCATGCCAAAAcc                           <  1:4193256/42‑1 (MQ=255)                                                                   |                                                            AGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCAATCTCAAGCGTCACCGGCGCTTCA  >  W3110S.gb/3101198‑3101323

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑