Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,136,226	T→C	D318G (GAT→GGT)	gss ←	fused glutathionylspermidine amidase and glutathionylspermidine synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,136,226	0	T	C	100.0%	309.2 / NA	88	D318G (GAT→GGT)	gss	fused glutathionylspermidine amidase and glutathionylspermidine synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (54/34);  total (54/34)

ACAGGAGGCGGAGTCGGCGTTGTACTCGTAAACCTTCAGGCCACGCTCATCCATGCAGAAATCCATACGACCAGTGATCATATGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGA  >  W3110S.gb/3136165‑3136292                                                              |                                                                   aCAGGAGGCGGAGTCGGCGTTGTACTCGTAAACCTTCAGGCCACGCTCATCCATGCAGAAACCCATACGAc                                                           >  1:2163938/1‑71 (MQ=255) aCAGGAGGCGGAGTCGGCGTTGTACTCGTAAACCTTCAGGCCACGCTCATCCATGCAGAAACCCATACGAc                                                           >  1:407211/1‑71 (MQ=255)      aggCGGAGTCGGCGTTGTACTCGTAAACCTTCAGGCCACGCTCATCCATGCAGAAACCCATACGACCAGt                                                       >  1:1926295/1‑70 (MQ=255)       ggCGGAGTCGGCGTTGTACTCGTAAACCTTCAGGCCACGCTCATCCATGCAGAAACCCATACGACCAGTGa                                                     >  1:680984/1‑71 (MQ=255)          ggAGTCGGCGTTGTACTCGTAAACCTTCAGGCCACGCTCATCCATGCAGAAACCCATACGACCAGTGATCa                                                  <  1:1226699/71‑1 (MQ=255)          ggAGTCGGCGTTGTACTCGTAAACCTTCAGGCCACGCTCATCCATGCAGAAACCCATACGACCAGTGATCa                                                  <  1:749108/71‑1 (MQ=255)          ggAGTCGGCGTTGTACTCGTAAACCTTCAGGCCACGCTCATCCATGCAGAAACCCATACGACCAGTGATCa                                                  <  1:3465744/71‑1 (MQ=255)           gAGTCGGCGTTGTACTCGTAAACCTTCAGGCCACGCTCATCCATGCAGAAACCCATACGACCAGTGATCa                                                  >  1:4417637/1‑70 (MQ=255)           gAGTCGGCGTTGTACTCGTAAACCTTCAGGCCACGCTCATCCATGCAGAAACCCATACGACCAGTGATCa                                                  <  1:2883646/70‑1 (MQ=255)            aGTCGGCGTTGTACTCGTAAACCTTCAGGCCACGCTCATCCATGCAGAAACCCATACGACCAGTGATCa                                                  <  1:1526794/69‑1 (MQ=255)            aGTCGGCGTTGTACTCGTAAACCTTCAGGCCACGCTCATCCATGCAGAAACCCATACGACCAGTGATCa                                                  <  1:3353633/69‑1 (MQ=255)            aGTCGGCGTTGTACTCGTAAACCTTCAGGCCACGCTCATCCATGCAGAAACCCATACGACCAGTGATCa                                                  <  1:1578106/69‑1 (MQ=255)              tCGGCGTTGTACTCGTAAACCTTCAGGCCACGCTCATCCATGCAGAAACCCATACGACCAGTGATCATATg                                              >  1:3680548/1‑71 (MQ=255)                ggCGTTGTACTCGTAAACCTTCAGGCCACGCTCATCCATGCAGAAACCCATACGACCAGTGATCATATGGt                                            >  1:4198259/1‑71 (MQ=255)                ggCGTTGTACTCGTAAACCTTCAGGCCACGCTCATCCATGCAGAAACCCATACGACCAGTGATCATATGGt                                            <  1:4142178/71‑1 (MQ=255)                ggCGTTGTACTCGTAAACCTTCAGGCCACGCTCATCCATGCAGAAACCCATACGACCAGTGATCATATGGt                                            >  1:352240/1‑71 (MQ=255)                 gCGTTGTACTCGTAAACCTTCAGGCCACGCTCATCCATGCAGAAACCCATACGACCAGTGATCa                                                  <  1:655130/64‑1 (MQ=255)                 gCGTTGTACTCGTAAACCTTCAGGCCACGCTCATCCATGCAGAAACCCATACGACCAGTGATCa                                                  <  1:1727037/64‑1 (MQ=255)                   gTTGTACTCGTAAACCTTCAGGCCACGCTCATCCATGCAGAAACCCATACGACCAGTGATCata                                                >  1:4585694/1‑64 (MQ=255)                   gTTGTACTCGTAAACCTTCAGGCCACGCTCATCCATGCAGAAACCCATACGACCAGTGATCATATGGTGAc                                         >  1:940999/1‑71 (MQ=255)                   gTTGTACTCGTAAACCTTCAGGCCACGCTCATCCATGCAGAAACCCATACGACCAGTGATCATATGGTGAc                                         >  1:1125218/1‑71 (MQ=255)                   gTTGTACTCGTAAACCTTCAGGCCACGCTCATCCATGCAGAAACCCATACGACCAGTCATCATATGGTGAc                                         <  1:4290879/71‑1 (MQ=255)                    ttGTACTCGTAAACCTTCAGGCCACGCTCATCCATGCAGAAACCCATACGACCAGTGAt                                                    <  1:3853350/59‑1 (MQ=255)                    ttGTACTCGTAAACCTTCAGGCCACGCTCATCCATGCAGAAACCCATACGACCAGTGAt                                                    <  1:130347/59‑1 (MQ=255)                    ttGTACTCGTAAACCTTCAGGCCACGCTCATCCATGCAGAAACCCATACGACCAGTGAt                                                    <  1:2185802/59‑1 (MQ=255)                    ttGTACTCGTAAACCTTCAGGCCACGCTCATCCATGCAGAAACCCATACGACCAGTGATCATATGGTg                                           <  1:316148/68‑1 (MQ=255)                    ttGTACTCGTAAACCTTCAGGCCACGCTCATCCATGCAGAAACCCATACGACCAGTGATCATATGGTGa                                          >  1:2084883/1‑69 (MQ=255)                      gTACTCGTAAACCTTCAGGCCACGCTCATCCATGCAGAAACCCATACGACCAGTGATCata                                                >  1:3344196/1‑61 (MQ=255)                      gTACTCGTAAACCTTCAGGCCACGCTCATCCATGCAGAAACCCATACGACCAGTGATCa                                                  >  1:1666053/1‑59 (MQ=255)                      gTACTCGTAAACCTTCAGGCCACGCTCATCCATGCAGAAACCCATACGACCAGTGATCATATGGTGACgg                                       >  1:4380693/1‑70 (MQ=255)                      gTACTCGTAAACCTTCAGGCCACGCTCATCCATGCAGAAACCCATACGACCAGTGATCATATGGTGACgg                                       >  1:294762/1‑70 (MQ=255)                      gTACTCGTAAACCTTCAGGCCACGCTCATCCATGCAGAAACCCATACGACCAGTGATCATATGGTGACgg                                       >  1:463075/1‑70 (MQ=255)                      gTACTCGTAAACCTTCAGGCCACGCTCATCCATGCAGAAACCCATACGACCAGTGATCATATGGTGACGGc                                      >  1:4180589/1‑71 (MQ=255)                      gTACTCGTAAACCTTCAGGCCACGCTCATCCATGCAGAAACCCATACGACCAGTGATCATATGGTGACGGc                                      >  1:3988944/1‑71 (MQ=255)                      gTACTCGTAAACCTTCAGGCCACGCTCATCCATGCAGAAACCCATACGACCAGTGATCATATGGTGACGGc                                      >  1:3940844/1‑71 (MQ=255)                      gTACTCGTAAACCTTCAGGCCACGCTCATCCATGCAGAAACCCATACGACCAGTGATCATATGGTGACGGc                                      >  1:1478463/1‑71 (MQ=255)                      gTACTCGTAAACCTTCAGGCCACGCTCATCCATGCAGAAACCCATACGACCAGTGATCATATGGTGACGGc                                      >  1:3498012/1‑71 (MQ=255)                      gTACTCGTAAACCTTCAGGCCACGCTCATCCATGCAGAAACCCATACGACCAGTGATCATATGGTGACGGc                                      >  1:4460550/1‑71 (MQ=255)                      gTACTCGTAAACCTTCAGGCCACGCTCATCCATGCAGAAACCCATACGACCAGTGATCATATGGTGACGGc                                      >  1:1939307/1‑71 (MQ=255)                      gTACTCGTAAACCTTCAGGCCACGCTCATCCATGCAGAAACCCATACGACCAGTGATCATATGGTGACGGc                                      >  1:3139735/1‑71 (MQ=255)                      gTACTCGTAAACCTTCAGGCCACGCTCATCCATGCAGAAACCCATACGACCAGTGATCATATGGTGACGGc                                      >  1:2650257/1‑71 (MQ=255)                      gTACTCGTAAACCTTCAGGCCACGCTCATCCATGCAGAAACCCATACGACCAGTGATCATATGGTGACGGc                                      >  1:2168925/1‑71 (MQ=255)                      gTACTCGTAAACCTTCAGGCCACGCTCATCCATGCAGAAACCCATACGACCAGTGATCATATGGTGACGGc                                      >  1:785995/1‑71 (MQ=255)                      gTACTCGTAAACCTTCAGGCCACGCTCATCCATGCAGAAACCCATACGACCAGTGATCATATGGTGACGGc                                      >  1:2042156/1‑71 (MQ=255)                         cTCGTAAACCTTCAGGCCACGCTCATCCATGCAGAAACCCATACGACCAGTGATCATATGGTGa                                          >  1:2217832/1‑64 (MQ=255)                         cTCGTAAACCTTCAGGCCACGCTCATCCATGCAGAAACCCATACGACCAGTGATCATATGGTGACGGCGAc                                   <  1:47195/71‑1 (MQ=255)                         cTCGTAAACCTTCAGGCCACGCTCATCCATGCAGAAACCCATACGACCAGTGATCATATGGTGACGGCGAc                                   <  1:2760055/71‑1 (MQ=255)                         cTCGTAAACCTTCAGGCCACGCTCATCCATGCAGAAACCCATACGACCAGTGATCATATGGTGACGGCGAc                                   <  1:1535391/71‑1 (MQ=255)                         cTCGTAAACCTTCAGGCCACGCTCATCCATGCAGAAACCCATACGACCAGTGATCATATGGTGACGGCGAc                                   <  1:2624725/71‑1 (MQ=255)                           cGTAAACCTTCAGGCCACGCTCATCCATGCAGAAACCCATACGACCAGTGATCATATGGTGACGGCGAc                                   >  1:4668079/1‑69 (MQ=255)                           cGTAAACCTTCAGGCCACGCTCATCCATGCAGAAACCCATACGACCAGTGATCATATGGTGACGGCGACg                                  >  1:572307/1‑70 (MQ=255)                           cGTAAACCTTCAGGCCACGCTCATCCATGCAGAAACCCATACGACCAGTGATCATATGGTGACGGCGACGc                                 >  1:4440294/1‑71 (MQ=255)                            gTAAACCTTCAGGCCACGCTCATCCATGCAGAAACCCATACGACCAGTGATCATATGGTGACGGCGACGCt                                >  1:1029402/1‑71 (MQ=255)                            gTAAACCTTCAGGCCACGCTCATCCATGCAGAAACCCATACGACCAGTGATCATATGGTGACGGCGACGCt                                >  1:304005/1‑71 (MQ=255)                             tAAACCTTCAGGCCACGCTCATCCATGCAGAAACCCATACGACCAGTGATCa                                                  <  1:2372911/52‑1 (MQ=255)                             tAAACCTTCAGGCCACGCTCATCCATGCAGAAACCCATACGACCAGTGATCa                                                  <  1:3988194/52‑1 (MQ=255)                               aaCCTTCAGGCCACGCTCATCCATGCAGAAACCCATAc                                                              >  1:3513915/1‑38 (MQ=255)                               aaCCTTCAGGCCACGCTCATCCATGCAGAAACCCATAc                                                              >  1:357621/1‑38 (MQ=255)                               aaCCTTCAGGCCACGCTCATCCATGCAGAAACCCATACGACCAGTGATCATATGGTGACGGCGACGCTGc                              >  1:4205183/1‑70 (MQ=255)                                 ccTTCAGGCCACGCTCATCCATGCAGAAACCCATACGACCAGTGATCATATGGTGACGGCGACGCTGCCAg                           <  1:4312032/71‑1 (MQ=255)                                  cTTCAGGCCACGCTCATCCATGCAGAAACCCATACGACCAGTGATCATATGGTGACGGCGACGCTGCCAg                           >  1:54341/1‑70 (MQ=255)                                      aGGCCAGGCTCATCCATGCAGAAACCCATACGACCAGTGATCATATGGTGACGGCGACGCTGCCAGgaga                       <  1:187468/70‑1 (MQ=255)                                      aGGCCACGCTCATCCATGCAGAAACCCATACGACCAGTGATCATATGGTGACGGCGACGCTGCCAGgaga                       <  1:1505871/70‑1 (MQ=255)                                          cACGCTCATCCATGCAGAAACCCATACGACCAGTGATCATATGGTGACGGCGACGCTGCCAGGAGAGACg                   >  1:1533171/1‑70 (MQ=255)                                           aCGCTCATCCATGCAGAAACCCATACGACCAGTGATCa                                                  <  1:1158464/38‑1 (MQ=255)                                              cTCATCCATGCAGAAACCCATACGACCAGTGATCATATGGTGACGGCGACGCTGCCAGGAGAGACGCAAAc              >  1:3806009/1‑71 (MQ=255)                                                cATCCATGCAGAAACCCATACGACCAGTGATCATATgga                                            >  1:817885/1‑38 (MQ=39)                                                  tCCATGCAGAAACCCATACGACCAGTGATCATATGGTGACGGc                                      <  1:667545/43‑1 (MQ=255)                                                  tCCATGCAGAAACCCATACGACCAGTGATCATATGGTGACGGc                                      <  1:3730774/43‑1 (MQ=255)                                                   ccATGCAGAAACCCATACGACCAGTGATCATATGGTGACGGc                                      <  1:1556871/42‑1 (MQ=255)                                                    cATGCAGAACCCCATACGACCAGTGATCATATGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGc         >  1:3068592/1‑70 (MQ=255)                                                    cATGCAGAAACCCATACGACCAGTGATCATATGGTGACGGCGa                                    >  1:1531997/1‑43 (MQ=255)                                                    cATGCAGAAACCCATACGACCAGTGATCATATGGTGACGGCGa                                    >  1:1486109/1‑43 (MQ=255)                                                    cATGCAGAAACCCATACGACCAGTGATCATATGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGc         >  1:4254979/1‑70 (MQ=255)                                                    cATGCAGAAACCCATACGACCAGTGATCATATGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGc         >  1:663291/1‑70 (MQ=255)                                                    cATGCAGAAACCCATACGACCAGTGATCATATGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGc         >  1:3898621/1‑70 (MQ=255)                                                    cATGCAGAAACCCATACGACCAGTGATCATATGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGc         >  1:333219/1‑70 (MQ=255)                                                    cATGCAGAAACCCATACGACCAGTGATCATATGGTGACGGCGACGCTGCCAGGAGAGACG‑AAACGTGGc         >  1:3715860/1‑69 (MQ=255)                                                       gCAGAAACCCATACGACCAGTGATCATATGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCa       >  1:1232911/1‑69 (MQ=255)                                                        cAGAAACCCATACGACCAGTGATCATATGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCagag    >  1:1531050/1‑71 (MQ=255)                                                        cAGAAACCCATACGACCAGTGATCATATGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCagag    >  1:2258030/1‑71 (MQ=255)                                                        cAGAAACCCATACGACCAGTGATCATATGGTGACGGCGACGCTGCCAGGAGAGACACAAACGTGGCCagag    >  1:3983770/1‑71 (MQ=255)                                                         aGAAACCCATACGACCAGTGATCATATGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAgg   <  1:3755822/71‑1 (MQ=255)                                                         aGAAACCCATACGACCAGTGATCATATGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAgg   <  1:1929986/71‑1 (MQ=255)                                                         aGAAACCCATACGACCAGTGATCATATGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAgg   <  1:375094/71‑1 (MQ=255)                                                          gAAACCCATACGACCAGTGATCATATGGTGACGGCGACGCTGCCAGgaga                       <  1:2928160/50‑1 (MQ=255)                                                          gAAACCCATACGACCAGTGATCATATGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGt            <  1:2999966/61‑1 (MQ=255)                                                           aaaCCCATACGACCAGTGATCATATGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGa  <  1:3960682/70‑1 (MQ=255)                                                              |                                                                   ACAGGAGGCGGAGTCGGCGTTGTACTCGTAAACCTTCAGGCCACGCTCATCCATGCAGAAATCCATACGACCAGTGATCATATGGTGACGGCGACGCTGCCAGGAGAGACGCAAACGTGGCCAGAGGA  >  W3110S.gb/3136165‑3136292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑