Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,222,206	C→T	N306N (AAC→AAT)	ebgA →	cryptic beta‑D‑galactosidase, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,222,206	0	C	T	100.0%	241.2 / NA	79	N306N (AAC→AAT)	ebgA	cryptic beta‑D‑galactosidase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (51/28);  total (51/28)

CAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGA  >  W3110S.gb/3222138‑3222273                                                                     |                                                                    cagcaATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGt                                                                   <  1:3000214/71‑1 (MQ=255)  agcaATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGtt                                                                  <  1:730061/71‑1 (MQ=255)  agcaATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGt                                                                   <  1:793111/70‑1 (MQ=255)        ggTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTgg                                                              >  1:1814381/1‑69 (MQ=255)         gTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTc                                                                 >  1:1201155/1‑65 (MQ=255)         gTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTgg                                                              >  1:415173/1‑68 (MQ=255)         gTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAg                                                           >  1:448255/1‑71 (MQ=255)         gTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGAAGCCAACGGCAATGTTc                                                                 >  1:769387/1‑65 (MQ=255)          tCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAgt                                                          <  1:1077928/71‑1 (MQ=255)          tCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAgt                                                          <  1:3891588/71‑1 (MQ=255)          tCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAgt                                                          <  1:2591154/71‑1 (MQ=255)          tCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAgt                                                          <  1:2470097/71‑1 (MQ=255)            agcagAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTc                                                                 <  1:4306882/62‑1 (MQ=255)                   tCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCcac                                                  >  1:1260539/1‑70 (MQ=255)                   tCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCcac                                                  >  1:4533023/1‑70 (MQ=255)                   tCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCcac                                                  >  1:4055911/1‑70 (MQ=255)                   tCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCcac                                                  >  1:4533583/1‑70 (MQ=255)                   tCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCcac                                                  >  1:2017343/1‑70 (MQ=255)                     cccTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACaa                                                >  1:3497203/1‑70 (MQ=255)                       cTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAAcgc                                             <  1:3740493/71‑1 (MQ=255)                         tATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGt                                           >  1:42048/1‑71 (MQ=255)                          aTCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGtt                                          >  1:386623/1‑71 (MQ=255)                          aTCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGtt                                          >  1:479090/1‑71 (MQ=255)                          aTCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGtt                                          >  1:4103266/1‑71 (MQ=255)                          aTCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGtt                                          >  1:4313463/1‑71 (MQ=255)                          aTCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGtt                                          >  1:4277627/1‑71 (MQ=255)                          aTCTTTACCATCTGGTCATGACGCTGAAAGACACCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGtt                                          >  1:828490/1‑71 (MQ=255)                           tCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGGAGTGGTGCCACAACGCGTTg                                         >  1:1558895/1‑71 (MQ=255)                               tACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTc                                                                 >  1:2726879/1‑43 (MQ=255)                               tACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCtt                                     >  1:2200658/1‑71 (MQ=255)                               tACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCtt                                     >  1:1636074/1‑71 (MQ=255)                               tACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCtt                                     >  1:2746535/1‑71 (MQ=255)                               tACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCtt                                     >  1:3364381/1‑71 (MQ=255)                               tACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCtt                                     >  1:2566919/1‑71 (MQ=255)                                   aTCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGt                                 <  1:1978877/71‑1 (MQ=255)                                   aTCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGt                                 <  1:4123928/71‑1 (MQ=255)                                   aTCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGt                                 <  1:3107798/71‑1 (MQ=255)                                   aTCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGt                                 <  1:1959789/71‑1 (MQ=255)                                    tCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAgtggt                                                       <  1:1316299/48‑1 (MQ=255)                                    tCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTcc                                   <  1:893630/68‑1 (MQ=255)                                    tCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGt                                 <  1:2735207/70‑1 (MQ=255)                                     cTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAgtggt                                                       <  1:629549/47‑1 (MQ=255)                                     cTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCca                                                   >  1:1994497/1‑51 (MQ=255)                                     cTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACaa                                                >  1:3254905/1‑54 (MQ=255)                                     cTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCt                                      >  1:2595731/1‑64 (MQ=255)                                     cTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGt                                 <  1:3393923/69‑1 (MQ=255)                                     cTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTg                                >  1:1927207/1‑70 (MQ=255)                                     cTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGa                               >  1:3039793/1‑71 (MQ=255)                                      tGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAgt                                                          <  1:2881043/43‑1 (MQ=255)                                      tGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAgt                                                          <  1:1909991/43‑1 (MQ=255)                                      tGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGa                               >  1:4402972/1‑70 (MQ=255)                                      tGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGa                               >  1:3208626/1‑70 (MQ=255)                                      tGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGa                               >  1:3014736/1‑70 (MQ=255)                                      tGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGa                               >  1:2426995/1‑70 (MQ=255)                                      tGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGa                               >  1:552179/1‑70 (MQ=255)                                      tGGACATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGa                               >  1:2394036/1‑70 (MQ=255)                                        gTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGa                               <  1:1123690/68‑1 (MQ=255)                                          cATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATc                           >  1:360153/1‑70 (MQ=255)                                          cATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATc                           >  1:4448261/1‑70 (MQ=255)                                           aTGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCaa                         <  1:1497999/71‑1 (MQ=255)                                           aTGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCa                          >  1:3573072/1‑70 (MQ=255)                                           aTGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCa                          >  1:1747590/1‑70 (MQ=255)                                           aTGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCa                          >  1:3039848/1‑70 (MQ=255)                                            tGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCaa                         <  1:997138/70‑1 (MQ=255)                                              aCGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGt                      >  1:4480728/1‑71 (MQ=255)                                              aCGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGt                      >  1:2001531/1‑71 (MQ=255)                                              aCGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGt                      >  1:1494978/1‑71 (MQ=255)                                              aCGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGt                      >  1:3858302/1‑71 (MQ=255)                                               cGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGt                      >  1:1160470/1‑70 (MQ=255)                                                    aaaGACGCCAACGGCAATGTTCTGGAAGTGGTG‑CACAACGCGTTGGCTTCCGTGATATCAAAGTGcgcg                 <  1:4573467/69‑1 (MQ=255)                                                      aGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACg              <  1:2256251/71‑1 (MQ=255)                                                      aGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACg              >  1:3323962/1‑71 (MQ=255)                                                            cAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGt        <  1:1937940/71‑1 (MQ=255)                                                              aCGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCa                          <  1:2178818/51‑1 (MQ=255)                                                              aCGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCa                          <  1:1556148/51‑1 (MQ=255)                                                                ggCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTg    >  1:958581/1‑71 (MQ=255)                                                                 gCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTg    >  1:2595532/1‑70 (MQ=255)                                                                  cAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGa  >  1:4514342/1‑71 (MQ=255)                                                                  cAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGa  >  1:4352028/1‑71 (MQ=255)                                                                     |                                                                    CAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGA  >  W3110S.gb/3222138‑3222273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑