Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,232,470	A→G	L111L (TTG→CTG)	ygjM ←	predicted DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,232,470	0	A	G	100.0%	192.7 / NA	56	L111L (TTG→CTG)	ygjM	predicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/32);  total (24/32)

GGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGATC  >  W3110S.gb/3232403‑3232536                                                                    |                                                                   ggAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAAc                                                                 >  1:1377908/1‑71 (MQ=255)     aaaTGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGt                                                             <  1:993773/71‑1 (MQ=255)     aaaTGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGt                                                             <  1:2412766/71‑1 (MQ=255)     aaaTGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGt                                                             <  1:4521995/71‑1 (MQ=255)     aaaTGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGt                                                             <  1:1399660/71‑1 (MQ=255)     aaaTGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGt                                                             <  1:2911642/71‑1 (MQ=255)     aaaTGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGt                                                             <  1:1540290/71‑1 (MQ=255)       aTGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGa                                                           >  1:209556/1‑71 (MQ=255)       aTGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGa                                                           >  1:2577762/1‑71 (MQ=255)       aTGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGa                                                           >  1:1646006/1‑71 (MQ=255)       aTGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGa                                                           >  1:2917344/1‑71 (MQ=255)       aTGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGa                                                           >  1:4431565/1‑71 (MQ=255)          ccGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGacac                                                        >  1:1399971/1‑71 (MQ=255)              aTCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATa                                                    <  1:80882/71‑1 (MQ=255)                 gcgTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGa                                                           >  1:3765850/1‑61 (MQ=255)                 gcgTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGAt                                                 >  1:559647/1‑71 (MQ=255)                   gTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGAttt                                               <  1:1145373/71‑1 (MQ=255)                   gTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGAtt                                                <  1:4307293/70‑1 (MQ=255)                      gCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACt                                            >  1:2021137/1‑71 (MQ=255)                      gCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACt                                            >  1:1517777/1‑71 (MQ=255)                           ttttttAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGacac                                                        <  1:1585952/54‑1 (MQ=255)                           ttttttAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGcc                                         <  1:4235665/69‑1 (MQ=255)                           ttttttAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGcc                                         <  1:1454028/69‑1 (MQ=255)                           ttttttAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCaa                                       <  1:2472080/71‑1 (MQ=255)                             ttttAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAAtt                                     >  1:761702/1‑71 (MQ=255)                             ttttAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACAATAGATTTACTGCCAAtt                                     >  1:2284223/1‑71 (MQ=255)                                     tgtTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAg                             <  1:2184592/71‑1 (MQ=255)                                     agtTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAg                             <  1:2664521/70‑1 (MQ=255)                                       ttCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCgg                                >  1:2167143/1‑66 (MQ=255)                                       ttCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCgg                                >  1:3542080/1‑66 (MQ=255)                                           aGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGa                       <  1:4090176/71‑1 (MQ=255)                                           aGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGa                       <  1:1163737/71‑1 (MQ=255)                                           aGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGa                       <  1:1689296/71‑1 (MQ=255)                                            gCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGa                       <  1:1268004/70‑1 (MQ=255)                                            gCGTTAATTTCCTCTTCCCGCTAAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGa                       <  1:2000892/70‑1 (MQ=255)                                              gTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCgg                                >  1:2870386/1‑59 (MQ=255)                                               ttAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGa                                                  <  1:2335178/40‑1 (MQ=255)                                                 aaTTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGt                 >  1:925239/1‑71 (MQ=255)                                                   tttCCTCTTCCCGCTCAGAACGCGTGACACCATAGAtt                                                <  1:765797/38‑1 (MQ=255)                                                   tttCCTCTTCCCGCTCAGAACGCGTGACACCATAGAtt                                                <  1:1026849/38‑1 (MQ=255)                                                   tttCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTa               <  1:1497693/71‑1 (MQ=255)                                                   tttCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTa               <  1:3147462/71‑1 (MQ=255)                                                     tCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTaaa             >  1:4678329/1‑71 (MQ=255)                                                       ctctTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAAc            >  1:3992029/1‑70 (MQ=255)                                                         ctTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCa          >  1:2818012/1‑70 (MQ=255)                                                          ttCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAg                    <  1:4049925/59‑1 (MQ=255)                                                          ttCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAg                    <  1:3612136/59‑1 (MQ=255)                                                          ttCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAg                    <  1:614959/59‑1 (MQ=255)                                                          ttCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAg                    <  1:3171642/59‑1 (MQ=255)                                                          ttCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCa          <  1:4541893/69‑1 (MQ=255)                                                            cccGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATAtt      >  1:666865/1‑71 (MQ=255)                                                            cccGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATAtt      >  1:801880/1‑71 (MQ=255)                                                            cccGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATAtt      >  1:3098754/1‑71 (MQ=255)                                                            cccGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATAtt      >  1:2260382/1‑71 (MQ=255)                                                                cTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGATc  <  1:1177745/71‑1 (MQ=255)                                                                cTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGATc  <  1:654921/71‑1 (MQ=255)                                                                    |                                                                   GGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGATC  >  W3110S.gb/3232403‑3232536

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑