Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,272,176	T→C	D146G (GAC→GGC)	garR ←	tartronate semialdehyde reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,272,176	0	T	C	100.0%	261.7 / NA	76	D146G (GAC→GGC)	garR	tartronate semialdehyde reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (39/37);  total (39/37)

ACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGC  >  W3110S.gb/3272109‑3272242                                                                    |                                                                   aCCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGCCg                                                                  >  1:4511739/1‑70 (MQ=255) aCCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGCCg                                                                  >  1:3032568/1‑70 (MQ=255) aCCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGCCg                                                                  >  1:1680739/1‑70 (MQ=255)  ccTGCACCGATTTCCGCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGCCGaa                                                                <  1:3052536/71‑1 (MQ=255)  ccTGCACCGATTTCCGCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGCCGaa                                                                <  1:3047195/71‑1 (MQ=255)  ccTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGCCGaa                                                                <  1:1510099/71‑1 (MQ=255)  ccTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGCCGa                                                                 <  1:1207708/70‑1 (MQ=255)      cACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGCCGaaaa                                                              >  1:2505368/1‑69 (MQ=255)       aCCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGCCGAAAATa                                                            <  1:106915/70‑1 (MQ=255)        ccGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGCCGAAAATAGc                                                          <  1:1330690/71‑1 (MQ=255)        ccGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGCCGAAAATAGc                                                          <  1:2251847/71‑1 (MQ=255)        ccGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGCCGAAAATAGc                                                          <  1:2119573/71‑1 (MQ=255)        ccGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGCCGAAAATAGc                                                          <  1:486286/71‑1 (MQ=255)        ccGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGCCGAAAATAGc                                                          <  1:1817712/71‑1 (MQ=255)           aTTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGCCGAAAATAGCCtt                                                       <  1:987087/71‑1 (MQ=255)           aTTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGCCGAAAATAGCCtt                                                       <  1:1279731/71‑1 (MQ=255)             ttCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGCCGAAAATAGCCTTGt                                                     >  1:4247098/1‑71 (MQ=255)                  cGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGCCGAAAATAGCCTTGTcgccg                                                >  1:3115022/1‑71 (MQ=255)                      aTGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGCCGaaaa                                                              >  1:430074/1‑53 (MQ=255)                      aTGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGCCGaaaa                                                              >  1:1940929/1‑53 (MQ=255)                      aTGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGCCGAAAATAGCCTTGTCGCCGCcca                                            >  1:3504767/1‑71 (MQ=255)                       tGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGCCGaaaa                                                              >  1:4661002/1‑52 (MQ=255)                       tGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGCCGAAAATAGCCTTGTCGCCGCccac                                           <  1:3100450/71‑1 (MQ=255)                       tGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGCCGAAAATAGCCTTGTCGCCGCccac                                           <  1:1089565/71‑1 (MQ=255)                       tGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGCCGAAAATAGCCTTGTCGCCGCccac                                           <  1:4011269/71‑1 (MQ=255)                         caccacGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGCCGaaaa                                                              >  1:1659544/1‑50 (MQ=255)                         caccacGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGCCGAAAATAGCCTTGTCGCCGCccacca                                         >  1:1608866/1‑71 (MQ=255)                         caccacGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGCCGAAAATAGCCTTGTCGCCGCccacca                                         >  1:3919751/1‑71 (MQ=255)                         caccacGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGCCGAAAATAGCCTTGTCGCCGCccacca                                         >  1:4004316/1‑71 (MQ=255)                         caccacGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGCCGAAAATAGCCTTGTCGCCGCccacca                                         >  1:431222/1‑71 (MQ=255)                            cacGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGCCGAAAATAGCCTTGTCGCCGCCCACCATCa                                      >  1:2210879/1‑71 (MQ=255)                            cacGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGCCGAAAATAGCCTTGTCGCCGCCCACCATCa                                      <  1:766198/71‑1 (MQ=255)                             acGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGCCGAAAATa                                                            >  1:2741794/1‑48 (MQ=255)                             acGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGCCGAAAATAGCCTTGTCGCCGCCCACCATc                                       >  1:911344/1‑69 (MQ=255)                              cGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGCCGAAAATAGCCTTGTCGCCGCCCACCATCACt                                    <  1:1868903/71‑1 (MQ=255)                              cGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGCCGAAAATAGCCTTGTCGCCGCCCACCATCACt                                    <  1:1167811/71‑1 (MQ=255)                              cGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGCCGAAAATAGCCTTGTCGCCGCCCACCATCACt                                    <  1:1952871/71‑1 (MQ=255)                              cGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGCCGAAAATAGCCTTGTCGCCGCCCACCATCACt                                    <  1:1543170/71‑1 (MQ=255)                                gAACCCGCCATCGCTTTCATCAAATCATAGTATTTGCCGaaaa                                                              >  1:3563726/1‑43 (MQ=255)                                 aaCCCGCCATCGCTTTCATCAAATCATAGTATTTGCCGAAAATAGCCTTGTCGCCGCCCACCATCACTGAc                                 <  1:211405/71‑1 (MQ=255)                                   cccGCCATCGCTTTCATCAAATCATAGTATTTGCCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAg                               <  1:1625161/71‑1 (MQ=255)                                     cGCCATCGCTTTCATCAAATCATAGTATTTGCCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCg                             <  1:2838404/71‑1 (MQ=255)                                      gCCATCGCTTTCATCAAATCATAGTATTTGCCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGt                            <  1:4675856/71‑1 (MQ=255)                                      gCCATCGCTTTCATCAAATCATAGTATTTGCCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGt                            <  1:3953076/71‑1 (MQ=255)                                          tCGTTTTCATCAAATCATAGTATTTGCCGAAAATAGCCtt                                                       <  1:935053/40‑1 (MQ=38)                                          tCGCTTTCATCAAATCATAGTATTTGCCGAAAATAGCCtt                                                       <  1:4424483/40‑1 (MQ=255)                                          tCGCTTTCATCAAATCATAGTATTTGCCGAAAATAGCCTTGTCGCCGCccac                                           <  1:31192/52‑1 (MQ=255)                                          tCGCTTTCATCAAATCATAGTATTTGCCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGt                            <  1:4052284/67‑1 (MQ=255)                                            gCTTTCATCAAATCATAGTATTTGCCGAAAATAGCCTTg                                                      >  1:4074749/1‑39 (MQ=255)                                            gCTTTCATCAAATCATAGTATTTGCCGAAAATAGCCTTGTCGCCGCccacca                                         >  1:3201262/1‑52 (MQ=255)                                            gCTTTCATCAAATCATAGTATTTGCCGAAAATAGCCTTGTCGCCGCCCACCATCACTGa                                  >  1:1738044/1‑59 (MQ=255)                                            gCTTTCATCAAATCATAGTATTTGCCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGt                       >  1:1490970/1‑70 (MQ=255)                                            gCTTTCATCAAATCATAGTATTTGCCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGt                       >  1:1255601/1‑70 (MQ=255)                                            gCTTTCATCAAATCATAGTATTTGCCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTc                      >  1:1043167/1‑71 (MQ=255)                                            gCTTTCATCAAATCATAGTATTTGCCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTc                      >  1:3668306/1‑71 (MQ=255)                                            gCTTTCATCAAATCATAGTATTTGCCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTc                      <  1:3208437/71‑1 (MQ=255)                                            gCTTTCATCAAATCATAGTATTTGCCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTc                      >  1:4059328/1‑71 (MQ=255)                                            gCTTTCATCAAATCATAGTATTTGCCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTc                      <  1:2727796/71‑1 (MQ=255)                                            gCTTTCATCAAATCATAGTATTTGCCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTc                      <  1:4269576/71‑1 (MQ=255)                                            gCTTTCATCAAATCATAGTATTTGCCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTc                      >  1:155740/1‑71 (MQ=255)                                            gCTTTCATCAAATCATAGTATTTGCCGAAAATAGCCTCGTCGCCGCCCACCATCACTGACAGCGTACCGTc                      >  1:4473241/1‑71 (MQ=255)                                               ttCATCAAATCATAGTATTTGCCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGAt                   >  1:3348227/1‑71 (MQ=255)                                                   tcaAATCATAGTATTTGCCGAAAATAGCCTTGTCGCCGCCCACCAt                                        <  1:1493344/46‑1 (MQ=255)                                                   tcaAATCATAGTATTTGCCGAAAATAGCCTTGTCGCCGCCCACCAt                                        <  1:1102612/46‑1 (MQ=255)                                                   tcaAATCATAGTATTTGCCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGc                <  1:2093272/70‑1 (MQ=255)                                                    caAATCATAGTATTTGCCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCtt              >  1:4445467/1‑71 (MQ=255)                                                    caAATCATAGTATTTGCCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCtt              >  1:4584533/1‑71 (MQ=255)                                                     aaaTCATAGTATTTGCCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGa                    >  1:2492550/1‑64 (MQ=255)                                                     aaaTCATAGTATTTGCCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGa                    >  1:2209524/1‑64 (MQ=255)                                                        tCATAGTATTTGCCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCgg          <  1:1922654/71‑1 (MQ=255)                                                         cATAGTATTTGCCGAAAATAGCCTTGTCGCCGCCCACCATCa                                      >  1:828551/1‑42 (MQ=255)                                                          aTAGTATTTGCCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGtt        >  1:4382997/1‑71 (MQ=255)                                                           tAGTATTTGCCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTc       <  1:333057/71‑1 (MQ=255)                                                                tttGCCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCAccgc  >  1:175091/1‑71 (MQ=255)                                                                tttGCCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCAccgc  >  1:4451851/1‑71 (MQ=255)                                                                tttGCCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCAccgc  >  1:2319778/1‑71 (MQ=255)                                                                    |                                                                   ACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGC  >  W3110S.gb/3272109‑3272242

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑