BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,324,704	G→A	L21L (CTC→CTT)	folP ←	7,8‑dihydropteroate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,324,704	0	G	A	100.0%	318.3 / NA	103	L21L (CTC→CTT)	folP	7,8‑dihydropteroate synthase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (37/66); total (37/66)

Only 100 of 103 total aligned reads displayed.

TGTTTCACCGCATCTATCAGCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGT  >  W3110S.gb/3324639‑3324771

tGTTTCACCGCATCTATCAGCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGAt                                                                >  1:4685069/1‑71 (MQ=255)

 gTTTCACCGCATCTATCAGCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATc                                                               >  1:4290721/1‑71 (MQ=255)

  tttCACCGCATCTATCAGCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATcc                                                              >  1:2586947/1‑71 (MQ=255)

  tttCACCGCATCTATCAGCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATcc                                                              >  1:2963465/1‑71 (MQ=255)

  tttCACCGCATCTATCAGCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATcc                                                              >  1:3162513/1‑71 (MQ=255)

  tttCACCGCATCTATCAGCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATc                                                               >  1:2075337/1‑70 (MQ=255)

  tttCACCGCATCTATCAGCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATc                                                               <  1:168844/70‑1 (MQ=255)

  tttCACCGCATCTATCAGCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATc                                                               <  1:113375/70‑1 (MQ=255)

  tttCACCGCATCTATCAGCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATc                                                               <  1:3330411/70‑1 (MQ=255)

    tCACCGCATCTATCAGCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGAGGTTAAGGATccc                                                             >  1:770624/1‑70 (MQ=255)

    tCACCGCATCTATCAGCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACTTTAAGGATccc                                                             <  1:3547076/70‑1 (MQ=255)

    tCACCGCATCTATCAGCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATccc                                                             <  1:1910782/70‑1 (MQ=255)

    tCACCGCATCTATCAGCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATccc                                                             <  1:337585/70‑1 (MQ=255)

    tCACCGCATCTATCAGCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATccc                                                             <  1:3624796/70‑1 (MQ=255)

        cGCATCTATCAGCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATCCCCATTa                                                        <  1:121316/71‑1 (MQ=255)

        cGCATCTATCAGCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATCCCCATTa                                                        <  1:1570836/71‑1 (MQ=255)

        cGCATCTATCAGCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATCCCCATTa                                                        <  1:4556481/71‑1 (MQ=255)

           aTCTATCAGCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATCCCCATTACGt                                                     <  1:926928/71‑1 (MQ=255)

            tCTATCAGCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATccc                                                             <  1:1922037/62‑1 (MQ=255)

            tCTATCAGCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATCCCCAt                                                          <  1:2150023/65‑1 (MQ=255)

            tCTATCAGCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATCCCCATTACGt                                                     >  1:2301980/1‑70 (MQ=255)

            tCTATCAGCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATCCCCATTACGt                                                     >  1:1046013/1‑70 (MQ=255)

            tCTATCAGCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATCCCCATTACGt                                                     >  1:1040610/1‑70 (MQ=255)

             cTATCAGCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATCCCCATTACGt                                                     >  1:2882222/1‑69 (MQ=255)

              tATCAGCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAgg                                                                  <  1:1035737/55‑1 (MQ=255)

              tATCAGCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATCCCCATTACGTGAg                                                  >  1:4170567/1‑71 (MQ=255)

              tATCAGCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATCCCCATTACGTGAg                                                  >  1:2312645/1‑71 (MQ=255)

              tATCAGCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATCCCCATTACGTGAg                                                  >  1:3596166/1‑71 (MQ=255)

              tATCAGCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATCCCCATTACGTGAg                                                  >  1:3098337/1‑71 (MQ=255)

              tATCAGCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATCCCCATTACGTGAg                                                  >  1:1827131/1‑71 (MQ=255)

              tATCAGCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATCCCCATTACGTGAg                                                  >  1:3145570/1‑71 (MQ=255)

              tATCAGCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATCCCCATTACGTGAg                                                  >  1:1353908/1‑71 (MQ=255)

              tATCAGCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATCCCCATTACGTGAg                                                  >  1:1332732/1‑71 (MQ=255)

                tCAGCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAgg                                                                  <  1:3516668/53‑1 (MQ=255)

                tCAGCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATCCCCATTACGTGAgg                                                 <  1:4152613/70‑1 (MQ=255)

                tCAGCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATCCCCATTACGTGAgg                                                 <  1:2523431/70‑1 (MQ=255)

                tCAGCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATCCCCATTACGTGAg                                                  <  1:2915633/69‑1 (MQ=255)

                tCAGCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATCCCCATTACGTGAg                                                  <  1:3206687/69‑1 (MQ=255)

                tCAGCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATCCCCATTACGTGAg                                                  <  1:3819525/69‑1 (MQ=255)

                tCAGCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATCCCCATTACGTGAg                                                  <  1:2723533/69‑1 (MQ=255)

                tCAGCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATCCCCATTACGTGAg                                                  <  1:2707883/69‑1 (MQ=255)

                tCAGCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATCCCCATTACGTGAg                                                  <  1:2660439/69‑1 (MQ=255)

                tCAGCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATCCCCATTACGTGAg                                                  <  1:2248747/69‑1 (MQ=255)

                tCAGCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATCCCCATTACGTGAg                                                  <  1:4317078/69‑1 (MQ=255)

                tCAGCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATCCCCATTACGTGAg                                                  <  1:1088841/69‑1 (MQ=255)

                tCAGCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATCCCCATTACGTGAg                                                  <  1:1106071/69‑1 (MQ=255)

                tCAGCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATCCCCATTACGTGAg                                                  <  1:13001/69‑1 (MQ=255)

                tCAGCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATCCCCATTACGTGAg                                                  <  1:136206/69‑1 (MQ=255)

                tCAGCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATCCCCATTACGTGAg                                                  <  1:629999/69‑1 (MQ=255)

                tCAGCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATCCCCATTACGTGAg                                                  <  1:462714/69‑1 (MQ=255)

                tCAGCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATCCCCATTACGTGAg                                                  <  1:2085523/69‑1 (MQ=255)

                tCAGCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATCCCCATTACGTGAg                                                  <  1:2516029/69‑1 (MQ=255)

                tCAGCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATCCCCATTACGTGAg                                                  <  1:2352463/69‑1 (MQ=255)

                tCAGCGAGTTATGCGTGCCACCATCCGAAAAGCAATCAGGCGTGACGTTAAGGATCCCCATTACGTGAg                                                  <  1:4247407/69‑1 (MQ=255)

                  aGCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATCCCCATTACGTGAGGAt                                               <  1:811706/70‑1 (MQ=255)

                  aGCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATCCCCATTACGTGAGGATg                                              >  1:1004640/1‑71 (MQ=255)

                   gCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATCCCCATTACGTGAg                                                  <  1:3497121/66‑1 (MQ=255)

                   gCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATCCCCATTACGTGAg                                                  <  1:3559623/66‑1 (MQ=255)

                     gAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATCCCCATTACGTGAGGATGGCt                                           <  1:509606/71‑1 (MQ=255)

                     gAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATCCCCATTACGTGAGGATGGCt                                           <  1:4644431/71‑1 (MQ=255)

                     gAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATCCCCATTACGTGAGGATGGCt                                           <  1:1888585/71‑1 (MQ=255)

                      aGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATCCCCATTACGTGAGGATGGCTa                                          <  1:4350589/71‑1 (MQ=255)

                      aGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATCCCCATTACGTGAGGATGGCTa                                          <  1:128476/71‑1 (MQ=255)

                        ttATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATcccc                                                            <  1:3880147/51‑1 (MQ=255)

                        ttATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATcccc                                                            <  1:3929292/51‑1 (MQ=255)

                          aTGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATCCCCATTACGTGAGGATGGCTAAGGt                                      >  1:161912/1‑71 (MQ=255)

                           tGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAgg                                                                  <  1:1005048/42‑1 (MQ=255)

                           tGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAgg                                                                  <  1:896157/42‑1 (MQ=255)

                           tGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAgg                                                                  <  1:1077085/42‑1 (MQ=255)

                           tGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAgg                                                                  <  1:3123882/42‑1 (MQ=255)

                             cGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGAt                                                                >  1:154748/1‑42 (MQ=255)

                             cGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTAAGGATCCCCATTACGTGAGGATGGCTAAGGTc                                     >  1:203336/1‑69 (MQ=255)

                               tGCCACCATCCGAAAAGGAATCAGGGGTGACGTTAAGGATCCCCATTACGTGAg                                                  <  1:1853862/54‑1 (MQ=255)

                                 ccaccaTCCGAAAAGGAATCAGGCGTGACGTTAAGGATCCCCATTACGTGAGGATGGCTAAg                                        >  1:1453308/1‑62 (MQ=255)

                                 ccaccaTCCGAAAAGGAATCAGGCGTGACGTTAAGGATCCCCATTACGTGAGGATGGCTAAg                                        >  1:872709/1‑62 (MQ=255)

                                 ccaccaTCCGAAAAGGAATCAGGCGTGACGTTAAGGATCCCCATTACGTGAGGATGGCTAAg                                        >  1:2362616/1‑62 (MQ=255)

                                 ccaccaTCCGAAAAGGAATCAGGCGTGACGTTAAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTg                                >  1:1856895/1‑70 (MQ=255)

                                 ccaccaTCCGAAAAGGAATCAGGCGTGACGTTAAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGa                               <  1:1863416/71‑1 (MQ=255)

                                    ccaTCCGAAAAGGAATCAGGCGTGACGTTAAGGATCCCCATTAc                                                       <  1:349415/44‑1 (MQ=255)

                                     caTCCGAAAAGGAATCAGGCGTGACGTTAAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTa                           <  1:2439931/71‑1 (MQ=255)

                                       tCCGAAAAGGAATCAGGCGTGACGTTAAGGATcccc                                                            <  1:4503176/36‑1 (MQ=255)

                                       tCCGAAAAGGAATCAGGCGTGACGTTAAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTAcc                         >  1:4227746/1‑71 (MQ=255)

                                         cGAAAAGGAATCAGGCGTGACGTTAAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTAcc                         >  1:2690491/1‑69 (MQ=255)

                                             aaGGAATCAGGCGTGACGTTAAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGaa                              >  1:2512239/1‑60 (MQ=255)

                                                 aaTCAGGCGTGACGTTAAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAag               <  1:1148608/71‑1 (MQ=255)

                                                     aGGCGTGACGTTAAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGtt           >  1:3524311/1‑71 (MQ=255)

                                                     aGGCGTGACGTTAAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGtt           >  1:4402679/1‑71 (MQ=255)

                                                         gTGACGTTAAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCat       <  1:4620996/71‑1 (MQ=255)

                                                         gTGACGTTAAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCat       <  1:947992/71‑1 (MQ=255)

                                                          tGACGTTAAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCatg      <  1:3781772/71‑1 (MQ=255)

                                                          tGACGTTAAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCat       <  1:580667/70‑1 (MQ=255)

                                                            aCGTTAAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGt                            >  1:597532/1‑47 (MQ=255)

                                                            aCGTTAAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTc         >  1:1648431/1‑66 (MQ=255)

                                                            aCGTTAAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCatgat    >  1:3142302/1‑71 (MQ=255)

                                                            aCGTTAAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCatga     >  1:2879934/1‑70 (MQ=255)

                                                             cGTTAAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCatgatg   <  1:4549599/71‑1 (MQ=255)

                                                              gTTAAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGt  <  1:2101665/71‑1 (MQ=255)

                                                              gTTAAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGt  <  1:448738/71‑1 (MQ=255)

                                                              gTTAAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGt  <  1:3239033/71‑1 (MQ=255)

                                                              gTTAAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGt  <  1:3728229/71‑1 (MQ=255)

TGTTTCACCGCATCTATCAGCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGT  >  W3110S.gb/3324639‑3324771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`