Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,343,341	T→C	I36T (ATC→ACC)	yhbN →	predicted transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,343,341	0	T	C	100.0%	282.2 / NA	82	I36T (ATC→ACC)	yhbN	predicted transporter subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/51);  total (31/51)

CTTGCCAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGTAATGTC  >  W3110S.gb/3343274‑3343408                                                                    |                                                                    cTTGCCAGCTCACTTCTGGCCGGCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCTGCCGACCca                                                                  <  1:2420145/71‑1 (MQ=255) cTTGCCAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCca                                                                  <  1:3775091/71‑1 (MQ=255) cTTGCCAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCca                                                                  <  1:2490856/71‑1 (MQ=255) cTTGCCAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCca                                                                  <  1:1705257/71‑1 (MQ=255)    gCCAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACAt                                                               >  1:205947/1‑71 (MQ=255)    gCCAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACAt                                                               >  1:500361/1‑71 (MQ=255)            aCTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCgg                                                       <  1:4340841/71‑1 (MQ=255)               tCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGAc                                                     >  1:3340083/1‑70 (MQ=255)                   gccgccAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGc                                                 <  1:66619/70‑1 (MQ=255)                   gccgccAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCa                                                <  1:2272291/71‑1 (MQ=255)                       ccAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAAtct                                            <  1:1594243/71‑1 (MQ=255)                         aGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATctct                                          <  1:2238849/71‑1 (MQ=255)                         aGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATctct                                          >  1:1895167/1‑71 (MQ=255)                          gCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCtt                                         <  1:3803222/71‑1 (MQ=255)                          gCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCtt                                         <  1:968917/71‑1 (MQ=255)                            aTTCCGGCATTTGCCGTAACCGTAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGa                                       <  1:265239/71‑1 (MQ=255)                            aTTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGTCCAGCAATCTCTTGa                                       <  1:2860479/71‑1 (MQ=255)                            aTTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGa                                       <  1:2238863/71‑1 (MQ=255)                            aTTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGa                                       <  1:872810/71‑1 (MQ=255)                            aTTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGa                                       <  1:4247102/71‑1 (MQ=255)                            aTTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGa                                       <  1:2759562/71‑1 (MQ=255)                            aTTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGa                                       <  1:2631273/71‑1 (MQ=255)                            aTTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGa                                       <  1:207538/71‑1 (MQ=255)                             ttCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCa                                                <  1:234853/61‑1 (MQ=255)                             ttCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCa                                                <  1:536120/61‑1 (MQ=255)                             ttCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGa                                       <  1:2692046/70‑1 (MQ=255)                             ttCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGa                                       <  1:627354/70‑1 (MQ=255)                               ccGGCATTTGCCGTAACCGGAGACACTGATCAGCCGCCCCACATTGAATCGGACCAGCAATCTCTTGata                                     >  1:608224/1‑70 (MQ=255)                               ccGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGa                                                      >  1:3843130/1‑53 (MQ=255)                               ccGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGatat                                    >  1:1202511/1‑71 (MQ=255)                               ccGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGatat                                    >  1:3715141/1‑71 (MQ=255)                               ccGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGatat                                    >  1:4502968/1‑71 (MQ=255)                               ccGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGatat                                    >  1:3508072/1‑71 (MQ=255)                               ccGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGatat                                    >  1:4340913/1‑71 (MQ=255)                               ccGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGata                                     >  1:55723/1‑70 (MQ=255)                               ccGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGata                                     >  1:4080824/1‑70 (MQ=255)                               ccGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGata                                     <  1:1114126/70‑1 (MQ=255)                               ccGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGata                                     >  1:1048318/1‑70 (MQ=255)                                  gCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTg                                                             <  1:2340377/43‑1 (MQ=255)                                  gCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCa                                 <  1:55676/71‑1 (MQ=255)                                  gCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCa                                 <  1:7625/71‑1 (MQ=255)                                     tttGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAAtctc                                           <  1:779930/58‑1 (MQ=255)                                       tGCCGTAACCGGAGACACTGATTAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAg                               >  1:672680/1‑68 (MQ=255)                                       tGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGc                             >  1:2869210/1‑70 (MQ=255)                                       tGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGc                             >  1:886765/1‑70 (MQ=255)                                       tGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGc                             >  1:4376863/1‑70 (MQ=255)                                       tGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGc                             >  1:4514929/1‑70 (MQ=255)                                       tGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGc                             >  1:4612809/1‑70 (MQ=255)                                       tGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGc                             >  1:1217958/1‑70 (MQ=255)                                           gTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGt                        <  1:4443137/71‑1 (MQ=255)                                            tAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGc                                                 <  1:2575291/45‑1 (MQ=255)                                            tAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGc                                                 <  1:4097819/45‑1 (MQ=255)                                            tAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTg                       <  1:3160739/71‑1 (MQ=255)                                              aCCGGAGACACTGATCAGCCGACCCACATTGAATCGGAc                                                     >  1:3334120/1‑39 (MQ=255)                                              aCCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGata                                     >  1:4013803/1‑55 (MQ=255)                                              aCCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGata                                     >  1:1984911/1‑55 (MQ=255)                                              aCCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGt                     <  1:1165193/71‑1 (MQ=255)                                                 ggAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTAc                  <  1:2789436/71‑1 (MQ=255)                                                     acacTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGa                                       <  1:1255908/46‑1 (MQ=255)                                                     acacTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCaa                           <  1:2774095/58‑1 (MQ=255)                                                     acacTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCaa                           <  1:3363044/58‑1 (MQ=255)                                                     acacTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCttt              <  1:633605/71‑1 (MQ=255)                                                       acTGATCAGCCGACCCACATTGAATCGGACCCGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTAc            >  1:2978737/1‑71 (MQ=255)                                                       acTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTAc            >  1:2260768/1‑71 (MQ=255)                                                         tGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTAcc                 >  1:4134766/1‑64 (MQ=255)                                                          gATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGata                                     >  1:4474663/1‑43 (MQ=255)                                                           aTCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGt        <  1:4589681/71‑1 (MQ=255)                                                           aTCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGt        <  1:4064378/71‑1 (MQ=255)                                                           aTCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGt        <  1:3986654/71‑1 (MQ=255)                                                           aTCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGt        <  1:2504059/71‑1 (MQ=255)                                                           aTCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGt        <  1:253697/71‑1 (MQ=255)                                                           aTCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGt        <  1:2730442/71‑1 (MQ=255)                                                           aTCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGt        <  1:3597476/71‑1 (MQ=255)                                                           aTCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGt        <  1:3479729/71‑1 (MQ=255)                                                            tCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGt        <  1:1885610/70‑1 (MQ=255)                                                            tCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGt        <  1:1500910/70‑1 (MQ=255)                                                             cAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGc                                  >  1:1872937/1‑43 (MQ=255)                                                             cAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGc                                  >  1:1126328/1‑43 (MQ=255)                                                             cAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTa                   >  1:351117/1‑58 (MQ=255)                                                              aGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGTAAt     <  1:2888559/71‑1 (MQ=255)                                                                 cGACCCACATTGAATCTGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGTAATGTc  <  1:224838/71‑1 (MQ=255)                                                                 cGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGTAATGTc  <  1:1888235/71‑1 (MQ=255)                                                                    |                                                                    CTTGCCAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGTAATGTC  >  W3110S.gb/3343274‑3343408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑