Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | W3110S.gb | 3,401,462 | T→C | I576V (ATT→GTT) | yhdA ← | conserved inner membrane protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | W3110S.gb | 3,401,462 | 0 | T | C | 100.0% | 103.0 / NA | 31 | I576V (ATT→GTT) | yhdA | conserved inner membrane protein |
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (13/18); total (13/18) |
GGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACT > W3110S.gb/3401394‑3401528 | ggTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAACGt < 1:4611930/71‑1 (MQ=255) gTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAACGTTTCTg < 1:1261217/69‑1 (MQ=255) aGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAACGTTTCTg > 1:1660968/1‑62 (MQ=255) gCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAACGTTTCTGACCAGCCCCg < 1:3578181/71‑1 (MQ=255) cTTCGACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAACGTTTCTGACCAGCCCCGGATgg < 1:2509594/71‑1 (MQ=255) cTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAACGTTTCTGACCAGCCCCGGATg > 1:4413992/1‑70 (MQ=255) caGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAACGttt < 1:72290/46‑1 (MQ=255) gCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAACGTTTCTGACCAGCCCCGGATGGAGCTTg < 1:1330748/67‑1 (MQ=255) gCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAACGTTTCTGACCAGCCCCGGATGGAGCTTg < 1:4547245/67‑1 (MQ=255) gCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAACGTTTCTGACCAGCCCCGGATGGAGCTTGAGt > 1:4600103/1‑70 (MQ=255) tttGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAACGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAAc > 1:1739757/1‑71 (MQ=255) aCCAGCAGCTGGTTCTCCGTTCGCTTCTCAACGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCaa < 1:353839/70‑1 (MQ=255) agcagcTGGTTCTCCGTTCGCTTCTCAACGTTTCTGa > 1:4060455/1‑37 (MQ=255) agcagcTGGTTCTCCGTTCGCTTCTCAACGTTTCTGa > 1:4591494/1‑37 (MQ=255) agcagcTGGTTCTCCGTTCGCTTCTCAACGTTTCTGa > 1:1398739/1‑37 (MQ=255) agcTGGTTCTCCGTTCGCTTCTCAACGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAg < 1:1199422/71‑1 (MQ=255) gatGGTTCTCCGTTCGCTTCTCAACGTTTCTGACCAG‑CCCGGATGGAGCTTGAGTAACTCAACATTAAGtt > 1:450440/3‑71 (MQ=255) ggTTCTCCGTTCGCTTCTCAACGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCt > 1:1266131/1‑71 (MQ=255) ttCTCCGTTCGCTTCTCAACGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCaa < 1:3347104/58‑1 (MQ=255) ctcCGTTCGCTTCTCAACGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTg < 1:2828711/70‑1 (MQ=255) ctcCGTTCGCTTCTCAACGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTg > 1:1465187/1‑70 (MQ=255) cGCTTCTCAACGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACt > 1:3245788/1‑71 (MQ=255) ttCTCAACGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCttt > 1:3706949/1‑59 (MQ=255) ttCTCAACGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTgg < 1:2002733/70‑1 (MQ=255) ctcAACGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGt < 1:4295050/69‑1 (MQ=255) ctcAACGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGt < 1:1079496/69‑1 (MQ=255) ctcAACGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGt < 1:4596138/69‑1 (MQ=255) ctcAACGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGt < 1:2004505/69‑1 (MQ=255) ctcAACGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGt < 1:967674/69‑1 (MQ=255) tcAACGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATcc < 1:68386/61‑1 (MQ=255) cAACGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACt > 1:3219855/1‑70 (MQ=255) | GGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACT > W3110S.gb/3401394‑3401528 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |