Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	495,162	G→A	E273E (GAG→GAA)	htpG →	molecular chaperone HSP90 family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	495,162	0	G	A	100.0%	114.3 / NA	39	E273E (GAG→GAA)	htpG	molecular chaperone HSP90 family
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/20);  total (19/20)

AAACACATCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACATCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATA  >  W3110S.gb/495094‑495223                                                                     |                                                              aaaCACATCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAATa                                                             <  1:3280902/71‑1 (MQ=255)  aaCACATCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAATa                                                             <  1:821043/70‑1 (MQ=255)  aaCACATCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAATa                                                             >  1:2195774/1‑70 (MQ=255)   acacATCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAATaca                                                           <  1:3035091/71‑1 (MQ=255)   acacATCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAATaca                                                           <  1:4279631/71‑1 (MQ=255)    cacaTCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAATacac                                                          >  1:1985351/1‑71 (MQ=255)    cacaTCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAATacac                                                          >  1:2831551/1‑71 (MQ=255)    cacaTCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAATaca                                                           >  1:4113361/1‑70 (MQ=255)     acaTCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAATacac                                                          >  1:569207/1‑70 (MQ=255)     acaTCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAATacac                                                          >  1:4615571/1‑70 (MQ=255)     acaTCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAATACAcc                                                         <  1:1943291/71‑1 (MQ=255)       aTCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAATACAcc                                                         <  1:1780695/69‑1 (MQ=255)          gCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAATACACCAGCct                                                    >  1:629368/1‑71 (MQ=255)              aCGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAATACACCAGCctgctg                                                <  1:1144020/71‑1 (MQ=255)              aCGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAATACACCAGCctgctg                                                <  1:2917003/71‑1 (MQ=255)               cGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAATa                                                             <  1:433086/57‑1 (MQ=255)                gACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAATACACCAGCCTGCTGTa                                              <  1:766184/71‑1 (MQ=255)                gACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAATACACCAGCCTGCTGTa                                              <  1:3968309/71‑1 (MQ=255)                gACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAATACACCAGCCTGCTGTa                                              <  1:1114925/71‑1 (MQ=255)                 aCTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAATACACCAg                                                       <  1:1201259/61‑1 (MQ=255)                 aCTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAATACACCAg                                                       <  1:4655389/61‑1 (MQ=255)                 aCTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAATACACCAGCCTGCTGTa                                              >  1:1494588/1‑70 (MQ=255)                    ttAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAATACACCAGCCTGCTGTACATc                                          >  1:2135080/1‑71 (MQ=255)                        tGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAATACACCAGCCTGCTGTa                                              >  1:2663837/1‑63 (MQ=255)                        tGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAATACACCAGCCTGCTGTACATCCCGt                                      >  1:2576922/1‑71 (MQ=255)                                      ggAGCCACAACCGTGTTGAAGGTAAGCAGGAATACACCAGCCTGCTGTACATCCCGTCCCAGGCTCCGTgg                        >  1:1044832/1‑71 (MQ=255)                                        aGCCACAACCGTGTTGAAGGTAAGCAGGAATACACCAGCCTGCTGTACATCCCGTCCCAGGCTCCGTGGGa                      <  1:3756874/71‑1 (MQ=255)                                        aGCCACAACCGTGTTGAAGGTAAGCAGGAATACACCAGCCTGCTGTACATCCCGTCCCAGGCTCCGTGGGa                      <  1:2521934/71‑1 (MQ=255)                                            acaACCGTGTTGAAGGTAAGCAGGAATACACCAGCCTGCTGTACATCCCGTCCCAGGCTCCGTGGGATAtg                  >  1:3698013/1‑71 (MQ=255)                                               aCCGTGTTGAAGGTAAGCAGGAATACACCAGCCTGCTGTACATCCCGTCCCAGGCTCCGTGGGata                    <  1:1577004/66‑1 (MQ=255)                                               aCCGTGTTGAAGGTAAGCAGGAATACACCAGCCTGCTGTACATCCCGTCCCAGGCTCCGTGGGATAtgtg                >  1:860295/1‑70 (MQ=255)                                                ccGTGTTGAAGGTAAGCAGGAATACACCAGCCTGCTGTACATCCCGTCCCAGGCTCCGTGGGa                      <  1:2698252/63‑1 (MQ=255)                                                     ttGAAGGTAAGCAGGAATACACCAGCCTGCTGTACATCCCGTCCCAGGCTCCGTGGGATATGTGGAACcgc         >  1:3935840/1‑71 (MQ=255)                                                            tAAGCAGGAATACACCAGCCTGCTGTACATCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCAt   >  1:4460826/1‑70 (MQ=255)                                                            tAAGCAGGAATACACCAGCCTGCTGTACATCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCAt   >  1:3537860/1‑70 (MQ=255)                                                            tAAGCAGGAATACACCAGCCTGCTGTACATCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATa  <  1:788523/71‑1 (MQ=255)                                                            tAAGCAGGAATACACCAGCCTGCTGTACATCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATa  <  1:3991112/71‑1 (MQ=255)                                                             aaGCAGGAATACACCAGCCTGCTGTACATCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATa  >  1:1732846/1‑70 (MQ=255)                                                             aaGCAGGAATACACCAGCCTGCTGTACATCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATa  >  1:457018/1‑70 (MQ=255)                                                                     |                                                              AAACACATCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACATCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATA  >  W3110S.gb/495094‑495223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑