Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,504,714	G→A	F711F (TTC→TTT)	metL ←	fused aspartokinase II and homoserine dehydrogenase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,504,714	0	G	A	100.0%	199.1 / NA	67	F711F (TTC→TTT)	metL	fused aspartokinase II and homoserine dehydrogenase II
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (41/26);  total (41/26)

CCCCATTTCGCGGGCCGCTTCCAGCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAGAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTCCACACGTACCTGATCCG  >  W3110S.gb/3504648‑3504778                                                                   |                                                                 ccccATTTCGCGGGCCGCTTCCAGCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAAt                                                              <  1:2111321/71‑1 (MQ=255)   ccATTTCGCGGGCCGCTTCCAGCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATgg                                                            <  1:2655226/71‑1 (MQ=255)     aTTTCGCGGGCCGCTTCCAGCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTc                                                          <  1:217226/71‑1 (MQ=255)     aTTTCGCGGGCCGCTTCCAGCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTc                                                          <  1:4645533/71‑1 (MQ=255)     aTTTCGCGGGCCGCTTCCAGCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTc                                                          <  1:1353242/71‑1 (MQ=255)     aTTTCGCGGGCCGCTTCCAGCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTc                                                          <  1:331720/71‑1 (MQ=255)     aTTTCGCGGGCCGCTTCCAGCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTc                                                          <  1:3775602/71‑1 (MQ=255)       ttCGCGGGCCGCTTCCAGCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGa                                                        >  1:1790077/1‑71 (MQ=255)        tCGCGGGCCGCTTCCAGCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATgg                                                            >  1:3849873/1‑66 (MQ=255)        tCGCGGGCCGCTTCCAGCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGa                                                        <  1:4298395/70‑1 (MQ=255)        tCGCGGGCCGCTTCCAGCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGAt                                                       >  1:893934/1‑71 (MQ=255)        tCGCGGGCCGCTTCCAGCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGAt                                                       >  1:512991/1‑71 (MQ=255)         cgcgGGCCGCTTCCAGCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGAt                                                       <  1:1708776/70‑1 (MQ=255)         cgcgGGCCGCTTCCAGCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGAt                                                       <  1:1583230/70‑1 (MQ=255)                cGCTTCCAGCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTgccgc                                               <  1:1186134/71‑1 (MQ=255)                    tCCAGCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAAt                                                              >  1:3690310/1‑52 (MQ=255)                            ttGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTc                                                          <  1:300773/48‑1 (MQ=255)                              gCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGt                                                           >  1:3931672/1‑45 (MQ=255)                              gCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGt                                                           >  1:4462403/1‑45 (MQ=255)                              gCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGt                                                           >  1:3117407/1‑45 (MQ=255)                              gCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCaa                                       >  1:1065112/1‑65 (MQ=255)                              gCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAg                                   >  1:2697191/1‑69 (MQ=255)                              gCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAg                                   >  1:1608441/1‑69 (MQ=255)                              gCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAg                                   >  1:2203807/1‑69 (MQ=255)                                aCCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAgg                               <  1:2350175/71‑1 (MQ=255)                                aCCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAgg                               <  1:47945/71‑1 (MQ=255)                                aCCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAgg                               <  1:4552774/71‑1 (MQ=255)                                aCCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAgg                               <  1:4434739/71‑1 (MQ=255)                                aCCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAgg                               <  1:4014094/71‑1 (MQ=255)                                aCCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAgg                               <  1:3139886/71‑1 (MQ=255)                                aCCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAgg                               <  1:3390661/71‑1 (MQ=255)                                aCCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAgg                               <  1:3261570/71‑1 (MQ=255)                                 ccATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAgg                               >  1:2707728/1‑70 (MQ=255)                                 ccATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAgg                               >  1:3818583/1‑70 (MQ=255)                                 ccATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGc                              <  1:1129815/71‑1 (MQ=255)                                  cATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCa                             >  1:2436605/1‑71 (MQ=255)                                   atatGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCa                             >  1:664203/4‑70 (MQ=255)                                      tGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGAtgctg                                                   >  1:3797487/1‑45 (MQ=255)                                      tGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCt                                             >  1:4510883/1‑51 (MQ=255)                                      tGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAg                         >  1:505840/1‑71 (MQ=255)                                      tGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAg                         >  1:3903085/1‑71 (MQ=255)                                           gttcagttcaTCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGa                      <  1:3019727/69‑1 (MQ=255)                                            ttcagttcaTCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTc                   <  1:2744751/71‑1 (MQ=255)                                              cagttcaTCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTCca                 >  1:2285662/1‑71 (MQ=255)                                                  tcaTCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTCca                 >  1:2012618/1‑67 (MQ=255)                                                   caTCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGa                                    >  1:4211163/1‑47 (MQ=255)                                                   caTCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTCca                 >  1:549105/1‑66 (MQ=255)                                                    aTCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTCCACACGt            <  1:3154037/70‑1 (MQ=255)                                                    aTCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTCCACACGTa           <  1:2865106/71‑1 (MQ=255)                                                       gCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTcc                  >  1:325655/1‑61 (MQ=255)                                                       gCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTCCACACGTACCt        >  1:1643083/1‑71 (MQ=255)                                                       gCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTCCACACGTACCt        >  1:103451/1‑71 (MQ=255)                                                          aTTTTCAAAAAAATGGTAGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTCCACACGTACCTGAt     >  1:1999809/1‑71 (MQ=255)                                                           ttttCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTc                   <  1:2829768/56‑1 (MQ=255)                                                             ttCGAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTCCACACGTACCTGATCCg  >  1:2883823/1‑71 (MQ=255)                                                             ttCAAAAAAATGGTCGATGTTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTCCACACGTACCTGATCCg  >  1:1206538/1‑71 (MQ=255)                                                             ttCAAAAAAATGGTCGATGCTGGCGCCTTCGCAATGAGCAGGCACCAGCGATTCca                 >  1:3368348/1‑56 (MQ=255)                                                             ttCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGc                                  >  1:4559648/1‑39 (MQ=255)                                                             ttCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCa                             >  1:1800960/1‑44 (MQ=255)                                                             ttCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGCTTCCACACGTACCTGATCCg  >  1:874161/1‑71 (MQ=255)                                                             ttCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGAtt                    >  1:3785706/1‑53 (MQ=255)                                                             ttCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTCCACACGTACCTGATCCg  >  1:4276541/1‑71 (MQ=255)                                                             ttCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTCCACACGTACCTGATCCg  >  1:1698868/1‑71 (MQ=255)                                                             ttCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTCCACACGTACCTGATCCg  >  1:171266/1‑71 (MQ=255)                                                             ttCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTCCACACGTACCTGATCCg  >  1:513580/1‑71 (MQ=255)                                                             ttCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTCCACACGTACCTGATCCg  >  1:1931950/1‑71 (MQ=255)                                                             ttCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTCCACACGTACCTGATCCg  >  1:780669/1‑71 (MQ=255)                                                                   |                                                                 CCCCATTTCGCGGGCCGCTTCCAGCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAGAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTCCACACGTACCTGATCCG  >  W3110S.gb/3504648‑3504778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑