Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,567,710	T→C	R102R (CGT→CGC)	yihQ →	alpha‑glucosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,567,710	0	T	C	100.0%	264.1 / NA	75	R102R (CGT→CGC)	yihQ	alpha‑glucosidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (54/21);  total (54/21)

TAATTCATTTCAGCCGTGGTTCTGACATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGTTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTGGCTGCGCCTTGCCGCTCAACCAG  >  W3110S.gb/3567643‑3567780                                                                    |                                                                       tAATTCATTTCAGCCGTGGTTCTGACATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTa                                                                     >  1:1397025/1‑71 (MQ=255) tAATTCATTTCAGCCGTGGTTCTGACATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTa                                                                     >  1:1934341/1‑71 (MQ=255) tAATTCATTTCAGCCGTGGTTCTGACATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTa                                                                     >  1:985118/1‑71 (MQ=255)  aaTTCATTTCAGCCGTGGTTCTGACATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTAt                                                                    >  1:4145968/1‑71 (MQ=255)   aTTCATTTCAGCCGTGGTTCTGACATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTAtt                                                                   <  1:3824418/71‑1 (MQ=255)   aTTCATTTCAGCCGTGGTTCTGACATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTAt                                                                    <  1:3471993/70‑1 (MQ=255)      cATTTCAGCCGTGGTTCTGACATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCt                                                                >  1:3542012/1‑71 (MQ=255)      cATTTCAGCCGTGGTTCTGACATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCt                                                                >  1:45497/1‑71 (MQ=255)      cATTTCAGCCGTGGTTCTGACATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCt                                                                >  1:2582942/1‑71 (MQ=255)      cATTTCAGCCGTGGTTCTGACATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCt                                                                >  1:3227921/1‑71 (MQ=255)      cATTTCAGCCGTGGTTCTGACATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCt                                                                >  1:4328029/1‑71 (MQ=255)        tttCAGCCGTGGTTCTGACATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCttg                                                              <  1:4338529/71‑3 (MQ=255)          tCAGCCGTGGTTCTGACATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGaa                                                            >  1:326034/1‑71 (MQ=255)          tCAGCCGTGGTTCTGACATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGa                                                             <  1:1356553/70‑1 (MQ=255)                   gTTCTGACATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACaaaa                                                    >  1:3232411/1‑70 (MQ=255)                   gTTCTGACATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAAc                                                   >  1:4301113/1‑71 (MQ=255)                     tCTGACATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGa                                                 >  1:3161309/1‑71 (MQ=255)                      cTGACATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGa                                                 >  1:4305845/1‑70 (MQ=255)                      cTGACATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGa                                                 >  1:3955054/1‑70 (MQ=255)                          cATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAAc                                             <  1:512348/70‑1 (MQ=255)                            ttAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTgg                                                              <  1:2312521/51‑1 (MQ=255)                            ttAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAATACGACAACCtt                                          <  1:2309832/71‑1 (MQ=255)                              aGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACaa                                              >  1:241989/1‑65 (MQ=255)                              aGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACaa                                              >  1:3702486/1‑65 (MQ=255)                              aGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCtt                                          >  1:2642601/1‑69 (MQ=255)                              aGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCt                                           >  1:1374640/1‑68 (MQ=255)                              aGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTaa                                        >  1:1863357/1‑71 (MQ=255)                              aGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTaa                                        >  1:2476652/1‑71 (MQ=255)                              aGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTaa                                        >  1:3205460/1‑71 (MQ=255)                              aGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTaa                                        >  1:3539956/1‑71 (MQ=255)                              aGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTaa                                        >  1:2580662/1‑71 (MQ=255)                              aGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTaa                                        >  1:284753/1‑71 (MQ=255)                              aGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTaa                                        >  1:2772504/1‑71 (MQ=255)                                       cTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCg                               >  1:4431736/1‑71 (MQ=255)                                       cTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCg                               >  1:2711217/1‑71 (MQ=255)                                       cTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCg                               <  1:1397788/71‑1 (MQ=255)                                       cTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCg                               >  1:4359588/1‑71 (MQ=255)                                       cTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCg                               >  1:1688586/1‑71 (MQ=255)                                       cTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCg                               <  1:1811467/71‑1 (MQ=255)                                       cTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAA‑CACAACCg                               >  1:2667485/1‑70 (MQ=255)                                          aaTATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTa                             <  1:2481000/70‑1 (MQ=255)                                           atatCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACcac                                    <  1:692505/62‑1 (MQ=255)                                            tatCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCtt                                          <  1:3107182/55‑1 (MQ=255)                                            tatCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCtt                                          <  1:1028285/55‑1 (MQ=255)                                            tatCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAAcc                                >  1:2544461/1‑65 (MQ=255)                                            tatCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCt                          >  1:1734455/1‑71 (MQ=255)                                            tatCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCt                          >  1:1211828/1‑71 (MQ=255)                                              tctcTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTac                                        >  1:1085991/1‑54 (MQ=255)                                              tctcTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTAt                            >  1:4659539/1‑67 (MQ=255)                                              tctcTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTgg                        >  1:3928976/1‑71 (MQ=255)                                              tctcTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTgg                        >  1:2776745/1‑71 (MQ=255)                                              tctcTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTgg                        >  1:4676352/1‑71 (MQ=255)                                              tctcTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTg                         <  1:1202464/70‑1 (MQ=255)                                              tctcTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACATTAACCACAACCGTATCTgg                        >  1:2675092/1‑71 (MQ=255)                                               ctctGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTGGc                       >  1:2477289/1‑71 (MQ=255)                                                tctGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGAc                                                >  1:3304406/1‑45 (MQ=255)                                                tctGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTGGCt                      >  1:138317/1‑71 (MQ=255)                                                tctGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTGGCt                      >  1:3177217/1‑71 (MQ=255)                                                    ccGACGATCAGGTGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTGGCTgcgc                  <  1:2511382/71‑1 (MQ=255)                                                    ccGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGa                                                 >  1:1596849/1‑40 (MQ=255)                                                    ccGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTGGCTgcg                   <  1:372291/70‑1 (MQ=255)                                                    ccGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTGGCTgcg                   <  1:3285967/70‑1 (MQ=255)                                                      gacgaTCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTGGCTGCGCCt                <  1:2723551/71‑1 (MQ=255)                                                      gacgaTCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTGGCTGCGCCt                <  1:4425350/71‑1 (MQ=255)                                                          aTCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTa                             <  1:1914594/54‑1 (MQ=255)                                                          aTCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTa                             <  1:1197476/54‑1 (MQ=255)                                                            cAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTg                         >  1:908563/1‑56 (MQ=255)                                                            cAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTGTCTGCGCCTTGCCGc          >  1:3520912/1‑71 (MQ=255)                                                            cAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTGGCTGCGCCTTGCCg           >  1:3994861/1‑70 (MQ=255)                                                            cAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTGGCTGCGCCTTGCCGc          >  1:2444380/1‑71 (MQ=255)                                                            cAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTGGCTGCGCCTTGCCGc          >  1:2535678/1‑71 (MQ=255)                                                            cAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTGGCTGCGCCTTGCCGc          >  1:4208855/1‑71 (MQ=255)                                                            cAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTGGCTGCGCCTTGCCGc          >  1:2914754/1‑71 (MQ=255)                                                            cAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCCGGCTGCGCCTTGCCGc          >  1:3781014/1‑71 (MQ=255)                                                                ggCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTGGCTGCGCCTTGCCGCTCaa      >  1:3898501/1‑71 (MQ=255)                                                                    tttATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTGGCTGCGCCTTGCCGCTCAACCag  <  1:15160/71‑1 (MQ=255)                                                                    tttATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTGGCTGCGCCTTGCCGCTCAACCag  <  1:2989212/71‑1 (MQ=255)                                                                    |                                                                       TAATTCATTTCAGCCGTGGTTCTGACATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGTTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTGGCTGCGCCTTGCCGCTCAACCAG  >  W3110S.gb/3567643‑3567780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑