Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,634,018	T→C	V46A (GTA→GCA)	yigF →	conserved inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,634,018	0	T	C	100.0%	266.4 / NA	78	V46A (GTA→GCA)	yigF	conserved inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/49);  total (29/49)

TCAACATGGTTTTCCTGGATTCTGGGGGGCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGTAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGATATATTTATTTG  >  W3110S.gb/3633950‑3634083                                                                     |                                                                  tCAACATGGTTTTCCTGGATTCTGGGGGGCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCaa                                                                 <  1:4645651/71‑1 (MQ=255)    aCATGGTTTTCCTGGATTCTGGGGGGCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGAc                                                              <  1:2039924/71‑1 (MQ=255)    aCATGGTTTTCCTGGATTCTGGGGGGCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGAc                                                              <  1:1935742/71‑1 (MQ=255)    aCATGGTTTTCCTGGATTCTGGGGGGCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGAc                                                              <  1:3774461/71‑1 (MQ=255)    aCATGGTTTTCCTGGATTCTGGGGGGCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGAc                                                              <  1:625222/71‑1 (MQ=255)            ttCCTGGATTCTGGGGGGCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCtt                                                       >  1:1102292/1‑70 (MQ=255)            ttCCTGGATTCTGGGGGGCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCtt                                                       <  1:3076721/70‑1 (MQ=255)            ttCCTGGATTCTGGGGGGCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCtt                                                       <  1:1561183/70‑1 (MQ=255)            ttCCTGGATTCTGGGGGGCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCtt                                                       <  1:429986/70‑1 (MQ=255)             tCCTGGATTCTGGGGGGCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCTTa                                                      <  1:2563417/70‑1 (MQ=255)             tCCTGGATTCTGGGGGGCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCTTa                                                      <  1:2795363/70‑1 (MQ=255)                    ttCTGGGGGGCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCTTAACTATTCa                                              <  1:794783/71‑1 (MQ=255)                    ttCTGGGGGGCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCTTAACTATTCa                                              <  1:1118822/71‑1 (MQ=255)                      cTGGGGGGCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCTTAACTa                                                  >  1:3420490/1‑65 (MQ=255)                         gggggCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATg                                          <  1:3225294/70‑1 (MQ=255)                         gggggCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATg                                          <  1:1759737/70‑1 (MQ=255)                         gggggCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATg                                          <  1:2256575/70‑1 (MQ=255)                         gggggCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATg                                          <  1:4665158/70‑1 (MQ=255)                         gggggCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATg                                          <  1:2455603/70‑1 (MQ=255)                             gCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATg                                          <  1:678295/66‑1 (MQ=255)                             gCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAAttt                                     <  1:124189/71‑1 (MQ=255)                             gCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAAttt                                     <  1:3352719/71‑1 (MQ=255)                              cAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGTATTTc                                    <  1:4621043/71‑1 (MQ=255)                                acacCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGAc                                                              <  1:2835274/43‑1 (MQ=255)                                acacCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCtt                                                       <  1:2897920/50‑1 (MQ=255)                                 cacCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCtt                                                       <  1:225526/49‑1 (MQ=255)                                 cacCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCtt                                                       <  1:615888/49‑1 (MQ=255)                                 cacCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCtt                                                       <  1:1667775/49‑1 (MQ=255)                                 cacCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCtt                                                       <  1:1613805/49‑1 (MQ=255)                                  acCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTg                                >  1:92069/1‑71 (MQ=255)                                  acCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTg                                >  1:3025607/1‑71 (MQ=255)                                   ccAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCTTAACTTTTCAAATGAATTTCATTGc                               <  1:35990/71‑1 (MQ=255)                                   ccAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGc                               >  1:1707753/1‑71 (MQ=255)                                     aGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCtt                             >  1:2211476/1‑71 (MQ=255)                                        atatAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTttct                          >  1:840479/1‑71 (MQ=255)                                        atatAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTttct                          >  1:699389/1‑71 (MQ=255)                                         tataAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCTTAGCTATTCAAATGAATTTCATTGCTttctt                         >  1:4239292/1‑71 (MQ=255)                                          ataAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTttctt                         <  1:4111303/70‑1 (MQ=255)                                           taAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCAtt                                 >  1:4224003/1‑61 (MQ=255)                                           taAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTtcttct                       >  1:897052/1‑71 (MQ=255)                                           taAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTtcttct                       >  1:696977/1‑71 (MQ=255)                                             aaGCAGCATTTAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGt                     >  1:1130207/1‑71 (MQ=255)                                             aaGCAGCATTTAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGt                     <  1:780272/71‑1 (MQ=255)                                              agcagcATTTAAAGCATTAAAAGCAAGACAACGCtt                                                       <  1:625975/36‑1 (MQ=255)                                              agcagcATTTAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGt                     <  1:1479288/70‑1 (MQ=255)                                              agcagcATTTAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGt                     <  1:3914722/70‑1 (MQ=255)                                              agcagcATTTAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGt                     <  1:3970418/70‑1 (MQ=255)                                                    aTTTAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGa              <  1:4059673/71‑1 (MQ=255)                                                     tttAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGt                     <  1:548926/63‑1 (MQ=255)                                                     tttAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGt                     <  1:1825760/63‑1 (MQ=255)                                                       tAAAGCATTAAACGCAAGACACCGCTTAACTATTCAAATGAATTTCATTGCt                              >  1:815092/1‑52 (MQ=37)                                                       tAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCAtt                                 >  1:1596682/1‑49 (MQ=255)                                                       tAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTtcttct                       >  1:284416/1‑59 (MQ=255)                                                       tAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGata            >  1:1708758/1‑70 (MQ=255)                                                       tAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGata            >  1:1952202/1‑70 (MQ=255)                                                       tAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGata            >  1:4169427/1‑70 (MQ=255)                                                       tAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGata            >  1:3013413/1‑70 (MQ=255)                                                       tAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGata            >  1:379255/1‑70 (MQ=255)                                                       tAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGata            >  1:3127909/1‑70 (MQ=255)                                                       tAAAGCATTAAAACCAAGACAACGCTTAACTATTCAAATGAATTTCAATGCTTTCTACTGTTCCt                 >  1:3558088/1‑65 (MQ=255)                                                        aaaGCATTAAAGGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGatata          >  1:1800831/1‑71 (MQ=255)                                                        aaaGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGatat           <  1:663978/70‑1 (MQ=255)                                                            cATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGATAtattta      >  1:3358953/1‑71 (MQ=255)                                                              ttAAAAGCAAGACAACTCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGATAtattta      <  1:2368863/69‑1 (MQ=255)                                                              ttAAAAGCAAGACAACTCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGATAtattta      <  1:2363580/69‑1 (MQ=255)                                                              ttAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGt                     <  1:2340053/54‑1 (MQ=255)                                                                aaaaGCAAGACAACGCTTAACTATTCAAATGAATTTCa                                   >  1:3476330/1‑38 (MQ=255)                                                                aaaaGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTttctt                         >  1:2951621/1‑48 (MQ=255)                                                                aaaaGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGATATATTTATTTg  >  1:55223/1‑71 (MQ=255)                                                                aaaaGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGATATATTTATTTg  >  1:2799910/1‑71 (MQ=255)                                                                 aaaGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGATATATTTATTTg  <  1:3298332/70‑1 (MQ=255)                                                                 aaaGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGATATATTTATTTg  <  1:2368277/70‑1 (MQ=255)                                                                 aaaGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGATATATTTATTTg  <  1:3518432/70‑1 (MQ=255)                                                                 aaaGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGATATATTTATTTg  <  1:541957/70‑1 (MQ=255)                                                                 aaaGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGATATATTTATTTg  <  1:2171433/70‑1 (MQ=255)                                                                 aaaGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGATATATTTATTTg  <  1:891246/70‑1 (MQ=255)                                                                 aaaGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGATATATTTATTTg  <  1:2969043/70‑1 (MQ=255)                                                                 aaaGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGATATATTTATTTg  <  1:3897962/70‑1 (MQ=255)                                                                     |                                                                  TCAACATGGTTTTCCTGGATTCTGGGGGGCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGTAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGATATATTTATTTG  >  W3110S.gb/3633950‑3634083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑