Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,679,788	G→T	intergenic (+34/+18)	ilvY → / ← ilvA	DNA‑binding transcriptional dual regulator/threonine deaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,679,788	0	G	T	100.0%	98.2 / NA	34	intergenic (+34/+18)	ilvY/ilvA	DNA‑binding transcriptional dual regulator/threonine deaminase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (17/17);  total (17/17)

AGGCATTCTGGAAGATTTTGCCGAACCACAAATGACGTTGTCGCGCGGGTAGGCCTGATAAGCGAAGCGCTATCAGGCATTTTTCCCTAACCCGCCAAAAAGAACCTGAACGCCGGGTTATTGGTTTCGT  >  W3110S.gb/3679720‑3679849                                                                     |                                                              aGGCATTCTGGAAGATTTTGCCGAACCACAAATGACGTTGTCGCGCGGGTAGGCCTGATAAGCGAAGctct                                                             <  1:268471/71‑1 (MQ=255)  ggCATTCTGGAAGATTTTGCCGAACCACAAATGACGTTGTCGCGCGGGTAGGCCTGATAAGCGAAGCTCTa                                                            <  1:1764918/71‑1 (MQ=255)     aTTCTGGAAGATTTTGCCGAACCACAAATGACGTTGTCGCGCGGGTAGGCCTGATAAGCGAAGCTCTa                                                            >  1:880638/1‑68 (MQ=255)        cTGGAAGATTTTGCCGAACCACAAATGACGTTGTCGCGCGGGTAGGCCTGATAAGCGAAGCTCTAt                                                           >  1:1653269/1‑66 (MQ=255)        cTGGAAGATTTTGCCGAACCACAAATGACGTTGTCGCGCGGGTAGGCCTGATAAGCGAAGCTCTATCAgg                                                       >  1:489049/1‑70 (MQ=255)        cTGGAAGATTTTGCCGAACCACAAATGACGTTGTCGCGCGGGTAGGCCTGATAAGCGAAGCTCTATCAgg                                                       >  1:3479866/1‑70 (MQ=255)        cTGGAAGATTTTGCCGAACCACAAATGACGTTGTCGCGCGGGTAGGCCTGATAAGCGAAGCTCTATCAgg                                                       >  1:3716987/1‑70 (MQ=255)        cTGGAAGATTTTGCCGAACCACAAATGACGTTGTCGCGCGGGTAGGCCTGATAAGCGAAGCTCTATCAgg                                                       >  1:3816839/1‑70 (MQ=255)        cTGGAAGATTTTGCCGAACCACAAATGACGTTGTCGCGCGGGTAGGCCTGATAAGCGAAGCTCTATCAgg                                                       >  1:1200648/1‑70 (MQ=255)        cTGGAAGATTTTGCCGAACCACAAATGACGTTGTCGCGCGGGTAGGCCTGATAAGCGAAGCTCTATCAgg                                                       >  1:2798266/1‑70 (MQ=255)        cTGGAAGATTTTGCCGAACCACAAATGACGTTGTCGCGCGGGTAGGCCTGATAAGCGAAGCTCTATCAgg                                                       >  1:4562312/1‑70 (MQ=255)        cTGGAAGATTTTGCCGAACCACAAATGACGTTGTCGCGCGGGTAGGCCTGATAAGCGAAGCTCTATCAgg                                                       >  1:2361113/1‑70 (MQ=255)        cTGGAAGATTTTGCCGAACCACAAATGACGTTGTCGCGCGGGTAGGCCTGATAAGCGAAGCTCTATCAgg                                                       >  1:1515138/1‑70 (MQ=255)        cTGGAAGATTTTGCCGAACCACAAATGACGTTGTCGCGCGGGTAGGCCTGATAAGCGAAGCTCTATCAgg                                                       >  1:1321933/1‑70 (MQ=255)        cTGGAAGATTTTGCCGAACCACAAATGACGTTGTCGCGCGGGTAGGCCTGATAAGCGAAGCTCTATCAgg                                                       >  1:939214/1‑70 (MQ=255)        cTGGAAGATTTTGCCGAACCACAAATGACGTTGTCGCGCGGGTAGGCCTGATAAGCGAAGCTCTATCAgg                                                       >  1:1249799/1‑70 (MQ=255)        cTGGAAGATTTTGCCGAACCACAAATGACGTTGTCGCGCGGGTAGGCCTGATAAGCGAAGCTATATCAgg                                                       >  1:3915820/1‑70 (MQ=255)           gAAGATTTTGCCGAACCACAAATGACGTTGTCGCGCGGGTAGGCCTGATAAGCGAAGCTCTATCAGGCa                                                     <  1:2397522/69‑1 (MQ=255)              gATTTTGCCGAACCACAAATGACGTTGTCGCGCGGGTAGGCCTGATAAGCGAAGCTCTATCAGGCAttttt                                                >  1:2534639/1‑71 (MQ=255)              gATTTTGCCGAACCACAAATGACGTTGTCGCGCGGGTAGGCCTGATAAGCGAAGCTCTATCAGGCAttttt                                                <  1:84276/71‑1 (MQ=255)              gATTTTGCCGAACCACAAATGACGTTGTCGCGCGGGTAGGCCTGATAAGCGAAGCTCTATCAGGCAttttt                                                <  1:2949636/71‑1 (MQ=255)                   tGCCGAACCACAAATGACGTTGTCGCGCGGGTAGGCCTGATAAGCGAAGCTCTATCAGGCATTTTTCCCt                                            <  1:2981167/70‑1 (MQ=255)                   tGCCGAACCACAAATGACGTTGTCGCGCGGGTAGGCCTGATAAGCGAAGCTCTATCAGGCATTTTTCCCt                                            <  1:2654306/70‑1 (MQ=255)                    gCCGAACCACAAATGACGTTGTCGCTCGGGTAGGCCTGATAAGCGAAGCTCTATCAGGCATTTTTCCCTa                                           <  1:4244431/70‑1 (MQ=38)                    gCCGAACCACAAATGACGTTGTCGCGCGGGTAGGCCTGATAAGCGAAGCTCTATCAGGCATTTTTCCCTa                                           <  1:2410678/70‑1 (MQ=255)                                 tGACGTTGTCGCGCGGGTAGGCCTGATAAGCGAAGCTCTATCAGGCATTTTTCCCTAACCCGCCAAAAAGa                             <  1:163488/71‑1 (MQ=255)                                      ttGTCGCGCGGGTAGGCCTGATAAGCGAAGCTCTTTCAGGCATTTTTCCCTAACCCGCCAAAAAGAACCTg                        <  1:63595/71‑1 (MQ=255)                                      ttGTCGCGCGGGTAGGCCTGATAAGCGAAGCTCTATCAGGCAtttt                                                 <  1:585795/46‑1 (MQ=255)                                      ttGTCGCGCGGGTAGGCCTGATAAGCGAAGCTCTATCAGGCATTTTTCCCTAACCCGCCAAAAAGAACCTg                        <  1:3474788/71‑1 (MQ=255)                                      ttGTCGCGCGGGTAGGCCTGATAAGCGAAGCTCTATCAGGCATTTTTCCCTAACCCGCCAAAAAGAACCTg                        <  1:2667338/71‑1 (MQ=255)                                      ttGTCGCGCGGGTAGGCCTGATAAGCGAAGCTCTATCAGGCATTTTTCCCTAACCCGCCAAAAAGAACCTg                        <  1:150893/71‑1 (MQ=255)                                        gTCGCGCGGGTAGGCCTGATAAGCGAAGCTCTATCAGGCATTTTTCCCTAACCCGCCAAAAAGAACCTGaa                      >  1:128038/1‑71 (MQ=255)                                                ggTAGGCCTGATAAGCGAAGCTCTATCAGGCATTTTTCCCTAACCCGCCAAAAAGAACCTGAACGCCGGGt              <  1:4624972/71‑1 (MQ=255)                                                    ggCCTGATAAGCGAAGCTCTATCAGGCATTTTTCCCTAACCCGCCAAAAAGAACCTg                        <  1:941480/57‑1 (MQ=255)                                                         gATAAGCGAAGCTCTATCAGGCATTTTTCCCTAACCCGCCAAAAAGAACCTg                        <  1:2997825/52‑1 (MQ=255)                                                            aagcgaagctCTATCAGGCATTTTTCCCTAACCCGCCAAAAAGAACCTGAACGCCGGGTTATTGGTTtcgt  >  1:3943201/1‑71 (MQ=255)                                                                     |                                                              AGGCATTCTGGAAGATTTTGCCGAACCACAAATGACGTTGTCGCGCGGGTAGGCCTGATAAGCGAAGCGCTATCAGGCATTTTTCCCTAACCCGCCAAAAAGAACCTGAACGCCGGGTTATTGGTTTCGT  >  W3110S.gb/3679720‑3679849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑