Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,712,785	T→C	V579A (GTA→GCA)	gidA →	glucose‑inhibited cell‑division protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,712,785	0	T	C	100.0%	122.7 / NA	37	V579A (GTA→GCA)	gidA	glucose‑inhibited cell‑division protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/25);  total (12/25)

GATGAGATCGAAAAGCAGCTGCGTAACGAGAACACCCTGCTACCCGCGACACTGGATTACCGCCAGGTATCCGGTCTTTCTAACGAAGTGATCGCCAAACTTAACGATCACAAACCAGCCTCTATCGGC  >  W3110S.gb/3712718‑3712846                                                                    |                                                              gATGAGATCGAAAAGCAGCTGCGTAACGAGAACACCCTGCTACCCGCGACACTGGATTACCGCCAGGCATc                                                            >  1:3098869/1‑71 (MQ=255)   tGAGATCGAAAAGCAGCTGCGTAACGAGAACACCCTGCTACCCGCGACACTGGATTACCGCCAGGCATCCg                                                          >  1:2372664/1‑71 (MQ=255)     agaTCGAAAAGCAGCTGCGTAACGAGAACACCCTGCTACCCGCGACACTGGATTACCGCCAGGCATCCGGt                                                        <  1:754784/71‑1 (MQ=255)     agaTCGAAAAGCAGCTGCGTAACGAGAACACCCTGCTACCCGCGACACTGGATTACCGCCAGGCATCCGGt                                                        <  1:546382/71‑1 (MQ=255)        tCGAAAAGCAGCTGCGTAACGAGAACACCCTGCTACCCGCGACACTGGATTACCGCCAGTCATCCGGTCtt                                                     <  1:3567485/71‑1 (MQ=255)                cagcTGCGTAACGAGAACACCCTGCTACCCGCGACACTGGATTACCGCCAGGCATCCGGTCTTTCTAACGa                                             <  1:657203/71‑1 (MQ=255)                 agcTGCGTAACGAGAACACCCTGCTACCCGCGACACTGGATTACCGCCAGGCATCCGGTCTTTCTAACGaa                                            <  1:3478839/71‑1 (MQ=255)                     gCGTAACGAGAACACCCTGCTACCCGCGACACTGGATTACCGCCAGGCATCCGGTCTTTCTAACGAAGTGa                                        >  1:4529232/1‑71 (MQ=255)                      cGTAACGAGAACACCCTGCTACCCGCGACACTGGATTACCGCCAGGCATCCGGTCTTTCTAACGAAGTg                                         <  1:3823796/69‑1 (MQ=255)                             agaACACCCTGCTACCCGCGACACTGGATTACCGCCAGGCATCCGGTCTTTCTAACGAAGTg                                         <  1:2892713/62‑1 (MQ=255)                                acacCCTGCTACCCGCGACACTGGATTACCGCCAGGCATCCGGTCTTTCTAACGAAGTGATCGCCAAACtt                             <  1:4229919/71‑1 (MQ=255)                                acacCCTGCTACCCGCGACACTGGATTACCGCCAGGCATCCGGTCTTTCTAACGAAGTGATCGCCAAACtt                             <  1:997135/71‑1 (MQ=255)                                 cacCCTGCTACCCGCGACACTGGATTACCGCCAGGCATCCGGTCTTTCTAACGAAGTGATCGCCAAACtt                             >  1:4030953/1‑70 (MQ=255)                                   cccTGCTACCCGCGACACTGGATTACCGCCAGGCATCCGGTCTTTCTAACGAAGTGATCGCCAAACTTAAc                          >  1:937976/1‑71 (MQ=255)                                    ccTGCTACCCGGGAGTCTGTATTACCGCAAGGCATCCGGTCTTTCTAActa                                             <  1:4623472/51‑3 (MQ=255)                                     atGCTACCCGCGACACTGGATTACCGCCAGGCATCCGGTCTTTCTAACGAAGTGATCGCCAAACTTAACGa                        >  1:950320/2‑71 (MQ=255)                                       gCTACCCGCGACACTGGATTACCGCCAGGCATCCGGTCTTTCTAACGAAGTg                                         <  1:3129500/52‑1 (MQ=255)                                           cccGCGACACTGGATTACCGCCAGGCATCCGGTCTTTCTAACGAAGTGATCGCCAAACTTAACGATcaca                   <  1:2026226/70‑1 (MQ=255)                                               cgACACTGGATTACCGCCAGGCATCCGGTCTTTCTAACGAAGTGATCGCCAAACTTAACGATCACAAACCa              <  1:3075191/71‑1 (MQ=255)                                                gACACTGGATTACCGCCAGGCATCCGGTCTTTCTAACGAAGTGATCGCCAAACTTAACGATCACAAACCAg             <  1:4373640/71‑1 (MQ=255)                                                 acacTGGATTACCGCCAGGCATCCGGTCTTTCTAACGAAGTGATCGCCAAACTTAACGATCACAAACCAGc            <  1:4644163/71‑1 (MQ=255)                                                 acacTGGATTACCGCCAGGCATCCGGTCTTTCTAACGAAGTGATCGCCAAACTTAACGATCACAAACCAGc            <  1:1792166/71‑1 (MQ=255)                                                  cacTGGATTACCGCCAGGCATCCGGTCTTTCTAACGAAGTGATCGCCAAACTTAACGATCACAAACCAGc            <  1:546895/70‑1 (MQ=255)                                                   acTGGATTACCGCCAGGCATCCGGTCTTTCTAACGAAGTGATCGCCAAACTTAACGATcaca                   <  1:3328354/62‑1 (MQ=255)                                                      ggATTACCGCCAGGCATCCGGTCTTTCTAACGAAg                                           >  1:2038115/1‑35 (MQ=255)                                                      ggATTACCGCCAGGCATCCGGTCTTTCTAACGAAg                                           >  1:1965369/1‑35 (MQ=255)                                                       gATTACCGCCAGGCATCCGGTCTTTCTAACGAAGTGATCGCCAAACTTAACGATCACAAACCAGCctct        <  1:1436718/69‑1 (MQ=255)                                                       gATTACCGCCAGGCATCCGGTCTTTCTAACGAAGTGATCGCCAAACTTAACGATCACAAACCAGCctct        <  1:3195391/69‑1 (MQ=255)                                                       gATTACCGCCAGGCATCCGGTCTTTCTAACGAAGTGATCGCCAAACTTAACGATCACAAACCAGCctct        <  1:3131863/69‑1 (MQ=255)                                                       gATTACCGCCAGGCATCCGGTCTTTCTAACGAAGTGATCGCCAAACTTAACGATCACAAACCAGCctct        <  1:200303/69‑1 (MQ=255)                                                       gATTACCGCCAGGCATCCGGTCTTTCTAACGAAGTGATCGCCAAACTTAACGATCACAAACCAGCctct        <  1:1685023/69‑1 (MQ=255)                                                       gATTACCGCCAGGCATCCGGTCTTTCTAACGAAGTGATCGCCAAACTTAACGATCACAAACCAGCCTCTa       <  1:4245353/70‑1 (MQ=255)                                                        aTTACCGCCAGGCATCCGGTCTTTCTAACGAAGTGATCGCCAAACTTAACGATCACAAACCAGCctct        <  1:2014446/68‑1 (MQ=255)                                                          tACCGCCAGGCATCCGGTCTTTCTAACGAAGTGATCGCCAAACTTAACGATCACAAACCAGcc           >  1:212888/1‑63 (MQ=255)                                                          tACCGCCAGGCATCCGGTCTTTCTAACGAAGTGATCGCCAAACTTAACGATCACAAACCAGCCTCTATCgg   >  1:893237/1‑71 (MQ=255)                                                          tACCGCCAGGCATCCGGTCTTTCTAACGAAGTGATCGCCAAACTTAACGATCACAAACCAGCCTATATCgg   >  1:1971792/1‑71 (MQ=255)                                                           aCCGCCAGGCATCCGGTCTTTCTAACGAAGTGATCGCCAAACTTAACGATCACAAACCAGCCTCTATCGGc  >  1:2682339/1‑71 (MQ=255)                                                                    |                                                              GATGAGATCGAAAAGCAGCTGCGTAACGAGAACACCCTGCTACCCGCGACACTGGATTACCGCCAGGTATCCGGTCTTTCTAACGAAGTGATCGCCAAACTTAACGATCACAAACCAGCCTCTATCGGC  >  W3110S.gb/3712718‑3712846

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑