Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,714,423	C→T	H61Y (CAC→TAC)	atpI →	ATP synthase, membrane‑bound accesory subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,714,423	0	C	T	100.0%	185.4 / NA	61	H61Y (CAC→TAC)	atpI	ATP synthase, membrane‑bound accesory subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/27);  total (34/27)

TCTCTGCAATAAGCGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCACCAGGCGCATACACCAGCGAAAGGCCGGGTGGCCTGGACATTCGCATTTGGCGAAGCTTTCAAAGT  >  W3110S.gb/3714355‑3714490                                                                     |                                                                    tctcTGCAATAAGCGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTAc                                                                   <  1:1940117/71‑1 (MQ=255)   tctGCACTAAGCGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTAc                                                                   >  1:592975/1‑69 (MQ=255)   tctGCAATAAGCGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTAc                                                                   >  1:1148355/1‑69 (MQ=255)   tctGCAATAAGCGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTAc                                                                   >  1:3591400/1‑69 (MQ=255)   tctGCAATAAGCGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTAc                                                                   >  1:3766316/1‑69 (MQ=255)   tctGCAATAAGCGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTAc                                                                   >  1:3199404/1‑69 (MQ=255)   tctGCAATAAGCGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTAc                                                                   >  1:3813512/1‑69 (MQ=255)   tctGCAATAAGCGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTAc                                                                   >  1:476574/1‑69 (MQ=255)       cAATAAGCGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGgcg                                                             <  1:1366979/71‑1 (MQ=255)         aTAAGCGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCa                                                           <  1:147284/71‑1 (MQ=255)          tAAGCGGGGGCCTGGGAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCa                                                           <  1:3021274/70‑1 (MQ=255)          tAAGCGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCa                                                           <  1:2479751/70‑1 (MQ=255)             gCGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATaca                                                       <  1:232304/71‑1 (MQ=255)                        gCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAgg                                            >  1:4338156/1‑71 (MQ=255)                         cAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGc                                           <  1:3224967/71‑1 (MQ=255)                           gTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCa                                                           <  1:4672320/53‑1 (MQ=255)                            tCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTTGCGTTAc                                                                   <  1:1277608/44‑1 (MQ=38)                            tCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTAc                                                                   <  1:3127039/44‑1 (MQ=255)                             cTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCa                                                           >  1:3361849/1‑51 (MQ=255)                              tttCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGt                                      >  1:2428871/1‑71 (MQ=255)                              tttCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGt                                      >  1:3563028/1‑71 (MQ=255)                              tttCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGt                                      >  1:3535542/1‑71 (MQ=255)                              tttCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGt                                      >  1:1426789/1‑71 (MQ=255)                              tttCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGt                                      >  1:2228510/1‑71 (MQ=255)                              tttCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGt                                      >  1:4257381/1‑71 (MQ=255)                              tttCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGt                                      >  1:4293617/1‑71 (MQ=255)                                tCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTAc                                                                   >  1:3845048/1‑40 (MQ=255)                                tCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTAc                                                                   >  1:3094101/1‑40 (MQ=255)                                tCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATa                                                         >  1:4219769/1‑50 (MQ=255)                                tCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGTgg                                    >  1:1837594/1‑71 (MQ=255)                                tCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGTgg                                    >  1:1804915/1‑71 (MQ=255)                                tCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGTgg                                    >  1:1781775/1‑71 (MQ=255)                                tCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGTgg                                    >  1:4588593/1‑71 (MQ=255)                                tCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGTg                                     <  1:4086787/70‑1 (MQ=255)                                    ccTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGTGGc                                   <  1:1186937/68‑1 (MQ=255)                                        aagTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGTGGCCTGGa                              <  1:4549468/67‑1 (MQ=255)                                        aCGTTTTGTTTATGATATTTGCCTGGCGTTACCAg                                                                >  1:783631/1‑35 (MQ=255)                                        aCGTTTTGTTTATGATATTTGCCTGGCGTTACCAg                                                                >  1:162194/1‑35 (MQ=255)                                        aCGTTTTGTTTATGATATTTGCCTGGCGTTACCAg                                                                >  1:1837276/1‑35 (MQ=255)                                        aCGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGTGGCCTGGa                              <  1:1339035/69‑1 (MQ=255)                                        aCGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGTGGCCTGGa                              <  1:4407065/69‑1 (MQ=255)                                        aCGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGTGGCCTGGACa                            >  1:884585/1‑71 (MQ=255)                                        aCGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGTGGCCTGGACa                            <  1:620075/71‑1 (MQ=255)                                        aCGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGTGGCCTGGACa                            >  1:4020885/1‑71 (MQ=255)                                        aCGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCACATACACCAGCGAAAGGCCGGGTGGCCTGGa                              <  1:3753276/69‑1 (MQ=255)                                          gTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGTGGCCTGGACAtt                          <  1:2921824/71‑1 (MQ=255)                                             ttGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGTGGCCTGGACATTCg                        <  1:3847166/70‑1 (MQ=255)                                             ttGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGTGGCCTGGACATTCg                        >  1:214660/1‑70 (MQ=255)                                               gTTTATGATATTTGCCTGGCGTTACCAGGCCCATACACCAGCGAAAGGCCGGGTGGCCTGGACATTCGCa                      <  1:4608166/70‑1 (MQ=255)                                                       tatTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGTGGCCTGGACATTCGCa                      <  1:1670958/62‑1 (MQ=255)                                                         tttGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGTGGCCTg                                >  1:1385362/1‑50 (MQ=255)                                                         tttGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGTGGCCTGGACATTCGCAtt                    >  1:3685869/1‑62 (MQ=255)                                                           tGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGTGGCCTGGACATTCGCATTTGGCGAAGCtt         >  1:4200201/1‑71 (MQ=255)                                                           tGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGTGGCCTGGACATTCGCATTTGGCGAAGCtt         >  1:1224252/1‑71 (MQ=255)                                                           tGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGTGGCCTGGACATTCGCATTTGGCGAAGCtt         >  1:3526649/1‑71 (MQ=255)                                                              cTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGTGGCCTGGACATTCGCATTTGGCGAAGCTTTCa      <  1:363317/71‑1 (MQ=255)                                                              cTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGTGGCCTGGACATTCGCATTTGGCGAAGCTTTCa      <  1:4633398/71‑1 (MQ=255)                                                              cTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGTGGCCTGGACATTCGCATTTGGCGAAGCTTTCa      <  1:2236426/71‑1 (MQ=255)                                                                 gCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGTGGCCTGGACATTCGCATTTGGCGAAGCTTTCaa     <  1:577449/69‑1 (MQ=255)                                                                  cGTTACCAGGCGCATACACCAGCGAAAGGCCGGGTGGCCTGGACATTCGCATTTGGCGAAGCTTTCAAAGt  <  1:1853118/71‑1 (MQ=255)                                                                  cGTTACCAGGCGCATACACCAGCGAAAGGCCGGGTGGCCTGGACATTCGCATTTGGCGAAGCTTTCAAAGt  <  1:2783948/71‑1 (MQ=255)                                                                     |                                                                    TCTCTGCAATAAGCGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCACCAGGCGCATACACCAGCGAAAGGCCGGGTGGCCTGGACATTCGCATTTGGCGAAGCTTTCAAAGT  >  W3110S.gb/3714355‑3714490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑