Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	505,179	C→T	L348L (CTG→TTG)	ushA →	bifunctional UDP‑sugar hydrolase and 5'‑nucleotidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	505,179	0	C	T	100.0%	212.0 / NA	70	L348L (CTG→TTG)	ushA	bifunctional UDP‑sugar hydrolase and 5'‑nucleotidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (38/32);  total (38/32)

GAAGACGGGAAAAGCGAGCGCGTGCTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGCTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATAGGCGAAACCAATGGTC  >  W3110S.gb/505113‑505245                                                                   |                                                                   gAAGACGGGAAAAGCGAGCGCGTGCTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCgttgtt                                                                <  1:2573379/71‑1 (MQ=255) gAAGACGGGAAAAGCGAGCGCGTGCTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCgttgtt                                                                <  1:187379/71‑1 (MQ=255)     aCGGGAAAAGCGAGCGCGTGCTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCa                                                            >  1:2488252/1‑71 (MQ=255)     aCGGGAAAAGCGAGAGCGTGCTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTAt                                                              >  1:3282480/1‑69 (MQ=255)      cGGGAAAAGCGAGCGCGTGCTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCAc                                                           <  1:86158/71‑1 (MQ=255)           aaaGCGAGCGCGTGCTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTc                                                      >  1:1947783/1‑71 (MQ=255)                gagcgCGTGCTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGaa                                                 <  1:1658637/71‑1 (MQ=255)                  gcgcGTGCTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACa                                               <  1:612266/71‑1 (MQ=255)                    gcgTGCTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGt                                                        <  1:371229/60‑1 (MQ=255)                         cTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCa                                         >  1:1423851/1‑70 (MQ=255)                         cTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCa                                         >  1:3924463/1‑70 (MQ=255)                         cTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCa                                         >  1:4234196/1‑70 (MQ=255)                         cTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCa                                         >  1:447706/1‑70 (MQ=255)                         cTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCa                                         >  1:4285346/1‑70 (MQ=255)                          tttACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCa                                         <  1:442992/69‑1 (MQ=255)                           ttACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAg                                      >  1:1301789/1‑71 (MQ=255)                            tACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTa                                                               >  1:3888145/1‑45 (MQ=255)                            tACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTa                                                               >  1:2290884/1‑45 (MQ=255)                            tACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTa                                                               >  1:1120096/1‑45 (MQ=255)                            tACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAgc                                     >  1:966463/1‑71 (MQ=255)                                cTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCAc                                                           <  1:2155771/45‑1 (MQ=255)                                cTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCAc                                                           <  1:3738066/45‑1 (MQ=255)                                cTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAg                                 >  1:879178/1‑71 (MQ=255)                                  ccTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTAt                                                              >  1:1632378/1‑40 (MQ=255)                                  ccTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTAt                                                              >  1:4150682/1‑40 (MQ=255)                                  ccTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCaa                                        >  1:4431806/1‑62 (MQ=255)                                  ccTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCaa                                        >  1:4656383/1‑62 (MQ=255)                                  ccTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAg                                      >  1:1646738/1‑64 (MQ=255)                                  ccTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCt                               >  1:1126427/1‑71 (MQ=255)                                  ccTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCt                               >  1:753443/1‑71 (MQ=255)                                  ccTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCt                               >  1:3300584/1‑71 (MQ=255)                                   cTGAAATCGCTGAAAACCAGCAAATGATCTCgttgtt                                                                <  1:4155832/37‑1 (MQ=255)                                   cTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTg                              >  1:3176167/1‑71 (MQ=255)                                   cTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTg                              >  1:4162792/1‑71 (MQ=255)                                     gAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTAt                                                              >  1:3224492/1‑37 (MQ=255)                                     gAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTAt                                                              >  1:2410471/1‑37 (MQ=255)                                     gAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTAt                                                              >  1:3356312/1‑37 (MQ=255)                                      aaaTCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTgg                             <  1:3026874/69‑1 (MQ=255)                                      aaaTCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTgg                             <  1:3212680/69‑1 (MQ=255)                                       aaTCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGt                                                        <  1:1523859/41‑1 (MQ=255)                                        aTCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGt                         >  1:1753219/1‑71 (MQ=255)                                         tCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGt                         >  1:2196764/1‑70 (MQ=255)                                         tCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGt                         >  1:4362094/1‑70 (MQ=255)                                          cGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTg                        >  1:147045/1‑70 (MQ=255)                                          cGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTg                        >  1:3800750/1‑70 (MQ=255)                                          cGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTg                        >  1:2373091/1‑70 (MQ=255)                                           gCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCTCAGCTGGAAGTGaa                      <  1:3394397/71‑1 (MQ=255)                                           gCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaa                      <  1:425684/71‑1 (MQ=255)                                           gCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaa                      <  1:1868419/71‑1 (MQ=255)                                           gCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaa                      <  1:537071/71‑1 (MQ=255)                                           gCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaa                      <  1:2102062/71‑1 (MQ=255)                                           gCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaa                      <  1:2222414/71‑1 (MQ=255)                                           gCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaa                      <  1:3630540/71‑1 (MQ=255)                                           gCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaa                      <  1:320887/71‑1 (MQ=255)                                               aaaaCCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaaaa                    <  1:829536/69‑1 (MQ=255)                                               aaaaCCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaaaa                    <  1:4437473/69‑1 (MQ=255)                                               aaaaCCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaaaa                    <  1:4553659/69‑1 (MQ=255)                                                aaaCCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATAg                 <  1:3820088/71‑1 (MQ=255)                                                   ccAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATAGGc               >  1:4044649/1‑70 (MQ=255)                                                      gCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaa                      <  1:2347711/60‑1 (MQ=255)                                                        aaaTGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATAGGCGAAAcc         <  1:1649933/71‑1 (MQ=255)                                                        aaaTGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATAGGCGAAAcc         <  1:3124614/71‑1 (MQ=255)                                                         aaTGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTgg                             <  1:1309237/50‑1 (MQ=255)                                                         aaTGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTgg                             <  1:1785470/50‑1 (MQ=255)                                                         aaTGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTgg                             <  1:1780153/50‑1 (MQ=255)                                                         aaTGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaa                      <  1:2048480/57‑1 (MQ=255)                                                          aTGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATAGGCGAAAcc         <  1:2615868/69‑1 (MQ=255)                                                             aTCTCGTTGTTATCACCTTTCCAGAACAAAGGCAAAGCGCAg                                 >  1:2997328/1‑42 (MQ=38)                                                             aTCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaa                      <  1:1180729/53‑1 (MQ=255)                                                             aTCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaa                      <  1:2419454/53‑1 (MQ=255)                                                             aTCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTAAAAATAGGCGAAACCAATgg    >  1:3298260/1‑71 (MQ=255)                                                             aTCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGATGGAAGTGAAAATAGGCGAAACCAATgg    >  1:2672497/1‑71 (MQ=255)                                                               ctcGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATAGGCGAAACCAATGgtc  >  1:1275701/1‑71 (MQ=255)                                                                   |                                                                   GAAGACGGGAAAAGCGAGCGCGTGCTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGCTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATAGGCGAAACCAATGGTC  >  W3110S.gb/505113‑505245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑