Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,803,370	A→C	L32R (CTT→CGT)	setC ←	predicted sugar efflux system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,803,370	0	A	C	100.0%	122.4 / NA	37	L32R (CTT→CGT)	setC	predicted sugar efflux system
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (17/20);  total (17/20)

TACCATTATAGGACGGGCTTTCAGTTCATCTGCGAGGAATATACTTAGGGTAGGAGTCTGAAGAGCGCCCGCAATACCCGTCAGAAAGGCGACAAGTAAAAATGCCGCGGCAGTGAGATCAAGTATTTT  >  W3110S.gb/3803309‑3803437                                                              |                                                                    tACCATTATAGGACGGGCTTTCAGTTCATCTGCGAGGAATATACTTAGGGTAGGAGTCTGACGAGCGCCCg                                                            >  1:3490331/1‑71 (MQ=255) tACCATTATAGGACGGGCTTTCAGTTCATCTGCGAGGAATATACTTAGGGTAGGAGTCTGACGAGCGCCCg                                                            >  1:1420695/1‑71 (MQ=255)   ccATTATAGGACGGGCTTTCAGTTCATCTGCGAGGAATATACTTAGGGTAGGAGTCTGACGAGCGCCCGc                                                           <  1:928465/70‑1 (MQ=255)      ttATAGGACGGGCTTTCAGTTCATCTGCGAGGAATATACTTAGGGTAGGAGTCTGACGAGCGCCCGCAATa                                                       <  1:1297088/71‑1 (MQ=255)      ttATAGGACGGGCTTTCAGTTCATCTGCGAGGAATATACTTAGGGTAGGAGTCTGACGAGCGCCCGCAATa                                                       <  1:2326448/71‑1 (MQ=255)               gggCTTTCAGTTCATCTGCGAGGAATATACTTAGGGTAGGAGTCTGACGAGCGCCCGCAATACCCGTCAGa                                              <  1:56097/71‑1 (MQ=255)                     tCAGTTCATCTGCGAGGAATATACTTAGGGTAGGAGTCTGACGAGCGCCCGCAATACCCGTCAGAAAGGc                                         <  1:1591631/70‑1 (MQ=255)                     tCAGTTCATCTGCGAGGAATATACTTAGGGTAGGAGTCTGACGAGCGCCCGCAATACCCGTCAGAAAGGc                                         >  1:3572149/1‑70 (MQ=255)                          tCATCTGCGAGGAATATACTTAGGGTAGGAGTCTGACGAGCGCCCGCAATACCCGTCAGAAAGGCGAc                                      >  1:1196571/1‑68 (MQ=255)                           cATCTGCGAGGAATATACTTAGGGTAGGAGTCTGACGAGCGCCCGCAATACCCGTCAGAAAGGCGACAAg                                   >  1:2121089/1‑70 (MQ=255)                               tGCGAGGAATATACTTAGGGTAGGAGTCTGACGAGCGCCCGCAATACCCGTCAGAAAGGCGACa                                     >  1:840333/1‑64 (MQ=255)                                 cGAGGAATATACTTAGGGTAGGAGTCTGACGAGCGCCCGCAATACCCGTCAGAAAGGCGACAAGTaaa                               <  1:3376838/68‑1 (MQ=255)                                 cGAGGAATATACTTAGGGTAGGAGTCTGACGAGCGCCCGCAATACCCGTCAGAAAGGCGACAAGTaaa                               <  1:200328/68‑1 (MQ=255)                                 cGAGGAATATACTTAGGGTAGGAGTCTGACGAGCGCCCGCAATACCCGTCAGAAAGGCGACAAGTaaa                               <  1:1993019/68‑1 (MQ=255)                                 cGAGGAATATACTTAGGGTAGGAGTCTGACGAGCGCCCGCAATACCCGTCAGAAAGGCGACAAGTaaa                               <  1:5121/68‑1 (MQ=255)                                 cGAGGAATATACTTAGGGTAGGAGTCTGACGAGCGCCCGCAATACCCGTCAGAAAGGCGACAAGTaaa                               <  1:1423778/68‑1 (MQ=255)                                 cGAGGAATATACTTAGGGTAGGAGTCTGACGAGCGCCCGCAATACCCGTCAGAAAGGCGACAAGTaa                                <  1:3578421/67‑1 (MQ=255)                                   aGGAATATACTTAGGGTAGGAGTCTGACGAGCGCCCGCAATACCCGTCAGAAAGGCGACAAGTAAAAATGc                          >  1:1890890/1‑71 (MQ=255)                                        tataCTTAGGGTAGGAGTCTGACGAGCGCCCGCAATACCCGTCAGAAAGGCGAc                                      <  1:915252/54‑1 (MQ=255)                                        tataCTTAGGGTAGGAGTCTGACGAGCGCCCGCAATACCCGTCAGAAAGGCGAc                                      <  1:107307/54‑1 (MQ=255)                                          taCTTAGGGTAGGAGTCTGACGAGCGCCCGCAATACCCGTCAGAAAGGCGACAAGTAAAAATGCCGCgg                     >  1:3689968/1‑69 (MQ=255)                                          taCTTAGGGTAGGAGTCTGACGAGCGCCCGCAATACCCGTCAGAAAGGCGACAAGTAAAAATGCCGCgg                     >  1:1833854/1‑69 (MQ=255)                                              tAGGGTAGGAGTCTGACGAGCGCCCGCAATACCCGTCAGAAAGGCGACAAGTAAAAATGCCGCGGCAGt                 >  1:3513713/1‑69 (MQ=255)                                              tAGGGTAGGAGTCTGACGAGCGCCCGCAATACCCGTCAGAAAGGCGACAAGTAAAAATGCCGCGGCAGt                 >  1:4002077/1‑69 (MQ=255)                                               aGGGTAGGAGTCTGACGAGCGCCCGCAATACCCGTCAGAAAGGCGACAAGTaaaaa                             >  1:2788621/1‑56 (MQ=255)                                               aGGGTAGGAGTCTGACGAGCGCCCGCAATACCCGTCAGAAAGGCGACAAGTAAAAATGCCGCGGCAGTg                <  1:1966854/69‑1 (MQ=255)                                                   tAGGAGTCTGACGAGCGCCCGCAATACCCGTCAGAAAGGCGACAAGTaaa                               <  1:2288112/50‑1 (MQ=255)                                                   tAGGAGTCTGACGAGCGCCCGCAATACCCGTCAGAAAGGCGACAAGTaaa                               <  1:4544140/50‑1 (MQ=255)                                                       aGTCTGACGAGCGCCCGCAATACCCGTCAGAAAGGCGACAAGTAAAAATGCCGCGGCAGTGAGATCAAGTa      >  1:1628544/1‑71 (MQ=255)                                                       aGTCTGACGAGCGCCCGCAATACCCGTCAGAAAGGCGACAAGTAAAAATGCCGCGGCAGTGAGATCAAGTa      <  1:2157201/71‑1 (MQ=255)                                                         tCTGACGAGCGCCCGCAATACCCGTCAGAAAGGCGACAAGTaaa                               <  1:1050758/44‑1 (MQ=255)                                                         tCTGACGAGCGCCCGCAATACCCGTCAGAAAGGCGACAAGTaaa                               <  1:579720/44‑1 (MQ=255)                                                         tCTGACGAGCGCCCGCAATACCCGTCAGAAAGGCGACAAGTaaa                               <  1:3660118/44‑1 (MQ=255)                                                           tGACGAGCGCCCGCAATACCCGTCAGAAAGGCGACAAGTAAAAATGCCGCGGCAGTga               >  1:136854/1‑58 (MQ=255)                                                           tGACGAGCGCCCGCAATACCCGTCAGAAAGGCGACAAGTAAAAATGCCGCGGCAGTGAGATCAAGTAtttt  >  1:4380714/1‑71 (MQ=255)                                                           tGACGAGCGCCCGCAATACCCGTCAGAAAGGCGACAAGTAAAAATGCCGCGGCAGTGAGATCAAGTAtttt  >  1:2381577/1‑71 (MQ=255)                                                           tGACGAGCGCCCGCAATACCCGTCAGAAAGGCGACAAGTAAAAATGCCGCGGCAGTGAGATCAAGTAtttt  >  1:344057/1‑71 (MQ=255)                                                              |                                                                    TACCATTATAGGACGGGCTTTCAGTTCATCTGCGAGGAATATACTTAGGGTAGGAGTCTGAAGAGCGCCCGCAATACCCGTCAGAAAGGCGACAAGTAAAAATGCCGCGGCAGTGAGATCAAGTATTTT  >  W3110S.gb/3803309‑3803437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑