BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,810,369	C→T	V368V (GTG→GTA)	yicE ←	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,810,369	0	C	T	100.0%	191.8 / NA	63	V368V (GTG→GTA)	yicE	predicted transporter
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (38/25); total (38/25)

GGTGCCAAACATTACAAGCGTTGCGCCGCCCAGAACCGGTTCTGGAATGTGTTGTACAAAACCGCTCACTGCCGGGAACAGACCCAGCACGATCAACATCAGCGCGACGACAAAACCGACATAGCGGCTGGCAA  >  W3110S.gb/3810303‑3810436

ggTGCCAAACATTACAAGCGTTGCGCCGCCCAGAACCGGTTCTGGAATGTGTTGTACAAAACCGCTTACTg                                                                 >  1:3021255/1‑71 (MQ=255)

ggTGCCAAACATTACAAGCGTTGCGCCGCCCAGAACCGGTTCTGGAATGTGTTGTACAAAACCGCTTACTg                                                                 >  1:2294382/1‑71 (MQ=255)

 gTGACAAACATTACAAGCGTTGCGCCGCCCAGAACCGGTTCTGGAATGTGTTGTACAAAACCGCTTACTg                                                                 <  1:560423/70‑1 (MQ=255)

     cAAACATTACAAGCGTTGCGCCGCCCAGAACCGGTTCTGGAATGTGTTGTACAAAACCGCTTACTGCCggg                                                            <  1:1748050/71‑1 (MQ=255)

      aaaCATTACAAGCGTTGCGCCGCCCAGAACCGGTTCTGGAATGTGTTGTACAAAACCGCTTACTGCCggg                                                            >  1:3943227/1‑70 (MQ=255)

         cATTACAAGCGTTGCGCCGCCCAGAACCCGTTCTGGAATGTGTTGTACAAAACCGCTTACTGCCGGGaa                                                          >  1:241445/1‑69 (MQ=255)

            tACAAGCGTTGCGCCGCCCAGAACCGGTTCTGGAATGTGTTGTACAAAACCGCTTACTGCCGGGAACAGa                                                      <  1:653529/70‑1 (MQ=255)

            tACAAGCGTTGCGCCGCCCAGAACCGGTTCTGGAATGTGTTGTACAAAACCGCTTACTGCCGGGAACAGAc                                                     >  1:1419375/1‑71 (MQ=255)

            tACAAGCGTTGCGCCGCCCAGAACCGGTTCTGGAATGTGTTGTACAAAACCGCTTACTGCCGGGAACAGAc                                                     >  1:2759395/1‑71 (MQ=255)

             aCAAGCGTTGCGCCGGCCAGAACCGGTTCTGGAATGTGTTGTACAAAACCGCTTACTGCCGGGAACAGAcc                                                    >  1:2625886/1‑71 (MQ=255)

             aCAAGCGTTGCGCCGCCCAGAACCGGTTCTGGAATGTGTTGTACAAAACCGCTTACTGCCGGGAACAGccc                                                    >  1:2912069/1‑71 (MQ=255)

             aCAAGCGTTGCGCCGCCCAGAACCGGTTCTGGAATGTGTTGTACAAAACCGCTTACTGCCGGGAACAGAcc                                                    >  1:4303768/1‑71 (MQ=255)

             aCAAGCGTTGCGCCGCCCAGAACCGGTTCTGGAATGTGTTGTACAAAACCGCTTACTGCCGGGAACAGAcc                                                    >  1:1923616/1‑71 (MQ=255)

             aCAAGCGTTGCGCCGCCCAGAACCGGTTCTGGAATGTGTTGTACAAAACCGCTTACTGCCGGGAACAGAcc                                                    >  1:4210329/1‑71 (MQ=255)

             aCAAGCGTTGCGCCGCCCAGAACCGGTTCTGGAATGTGTTGTACAAAACCGCTTACTGCCGGGAACAGAcc                                                    >  1:2062454/1‑71 (MQ=255)

             aCAAGCGTTGCGCCGCCCAGAACCGGTTCTGGAATGTGTTGTACAAAACCGCTTACTGCCGGGAACAGAcc                                                    >  1:3659768/1‑71 (MQ=255)

             aCAAGCCTTGCGCCGCCCAGAACCGGTTCTGGAATGTGTTGTACAAAACCGCTTACTGCCGGGAACAGAcc                                                    >  1:593672/1‑71 (MQ=255)

                aGCGTTGCGCCGCCCAGAACCGGTTCTGGAATGTGTTGTACAAAACCGCTTACTGCCGGGAACAGACCCAg                                                 >  1:109068/1‑71 (MQ=255)

                      gcgcCGCCCAGAACCGGTTCTGGAATGTGTTGTACAAAACCGCTTACTGCCGGGAACAGACCCAGCACGa                                            >  1:3074866/1‑70 (MQ=255)

                       cgccgcCCAGAACCGGTTCTGGAATGTGTTGTACAAAACCGCTTACTGCCGGGAACAGACCCAGCACGATc                                          <  1:621361/71‑1 (MQ=255)

                       cgccgcCCAGAACCGGTTCTGGAATGTGTTGTACAAAACCGCTTACTGCCGGGAACAGACCCAGCACGATc                                          <  1:3291594/71‑1 (MQ=255)

                         ccgccCAGAACCGGTTCTGGAATGTGTTGTACAAAACCGCTTACTGCCGGGAACAGACCCAGCACGATCaa                                        <  1:839209/71‑1 (MQ=255)

                            cccAGAACCGGTTCTGGAATGTGTTGTACAAAACCGCTTACTGCCGGGaa                                                          <  1:2946298/50‑1 (MQ=255)

                              cAGAACCGGTTCTGGAATGTGTTGTACAAAACCGCTTACTGCCGGGAACAGACCCAGCACGATCAACAt                                     >  1:1485875/1‑69 (MQ=255)

                                 aaCCGGTTCTGGAATGTGTTGTACAAAACCGCTTACTGCCGGGAACAGACCCAGCACGATCAACATCAgcg                                >  1:2448663/1‑71 (MQ=255)

                                  aCCGGTTCTGGAATGTGTTGTACAAAACCGCTTACTGc                                                                >  1:251806/1‑38 (MQ=255)

                                  aCCGGTTCTGGAATGTGTTGTACAAAACCGCTTACTGCCGGGaa                                                          >  1:3366955/1‑44 (MQ=255)

                                  aCCGGTTCTGGAATGTGTTGTACAAAACCGCTTACTGCCGGGaa                                                          >  1:3036304/1‑44 (MQ=255)

                                  aCCGGTTCTGGAATGTGTTGTACAAAACCGCTTACTGCCGGGAACAGACCCAGCACGAt                                           >  1:4662961/1‑59 (MQ=255)

                                  aCCGGTTCTGGAATGTGTTGTACAAAACCGCTTACTGCCGGGAACAGACCCAGCACGATCAACATCAgcgc                               >  1:4462579/1‑71 (MQ=255)

                                   ccGGTTCTGGAATGTGTTGTACAAAACCGCTTACTGCCGGGAACAGACCCAGCACGATCAACATCAgcgc                               >  1:2315807/1‑70 (MQ=255)

                                         cTGGAATGTGTTGTACAAAACCGCTTACTGCCGGGAACAGACCCAGCACGATCAACATCAGCGCgacgac                         >  1:3228325/1‑70 (MQ=255)

                                            gAACGTGTTGTACAAAACCGCTTACTGCCGGGAACAGACCCAGCACGATCAACATCAGCGCGACGACa                        <  1:1086063/68‑1 (MQ=255)

                                             aaTGTGTTGTACAAAACCGCTTACTGCCGGGAACAGACCCAGCACGATCAACATCAGCGCGACGACAAAAc                    >  1:4441930/1‑71 (MQ=255)

                                              aTGTGTTGTACAAAACCGCTTACTGCCGGGAACAGACCCAGCACGATCAACATCAGCGCGACGACAAAAcc                   <  1:2929306/71‑1 (MQ=255)

                                               tgtgTTGTACAAAACCGCTTACTGCCGGGAACAGACCCAGCACGATCAACATCAGCGCGACGACAAAACCg                  <  1:1763587/71‑1 (MQ=255)

                                                 tgttgtACAAAACCGCTTACTGCCGGGAACAGACCCAGCACGATCAACAt                                     >  1:1827250/1‑50 (MQ=255)

                                                 tgttgtACAAAACCGCTTACTGCCGGGAACAGACCCAGCACGATCAACATCa                                   >  1:3154067/1‑52 (MQ=255)

                                                 tgttgtACAAAACCGCTTACTGCCGGGAACAGACCCAGCACGATCAACATCAg                                  >  1:393386/1‑53 (MQ=255)

                                                 tgttgtACAAAACCGCTTACTGCCGGGAACAGACCCAGCACGATCAACATCAGCGCGACGACAAAACCGa                 <  1:2998567/70‑1 (MQ=255)

                                                 tgttgtACAAAACCGCTTACTGCCGGGAACAGACCCAGCACGATCAACATCAGCGCGACGACAAAACCGa                 <  1:1172429/70‑1 (MQ=255)

                                                 tgttgtACAAAACCGCTTACTGCCGGGAACAGACCCAGCACGATCAACATCAGCGCGACGACAAAACCGa                 >  1:592329/1‑70 (MQ=255)

                                                 tgttgtACAAAACCGCTTACTGCCGGGAACAGACCCAGCACGATCAACATCAGCGCGACGACAAAACCGa                 >  1:566235/1‑70 (MQ=255)

                                                 tgttgtACAAAACCGCTTACTGCCGGGAACAGACCCAGCACGATCAACATCAGCGCGACGACAAAACCGa                 <  1:485856/70‑1 (MQ=255)

                                                 tgttgtACAAAACCGCTTACTGCCGGGAACAGACCCAGCACGATCAACATCAGCGCGACGACAAAACCGa                 >  1:4475076/1‑70 (MQ=255)

                                                 tgttgtACAAAACCGCTTACTGCCGGGAACAGACCCAGCACGATCAACATCAGCGCGACGACAAAACCGa                 >  1:4068388/1‑70 (MQ=255)

                                                 tgttgtACAAAACCGCTTACTGCCGGGAACAGACCCAGCACGATCAACATCAGCGCGACGACAAAACCGa                 <  1:2152574/70‑1 (MQ=255)

                                                 tgttgtACAAAACCGCTTACTGCCGGGAACAGACCCAGCACGATCAACATCAGCGCGACGACAAAACCGa                 <  1:2300401/70‑1 (MQ=255)

                                                 tgttgtACAAAACCGCTTACTGCCGGGAACAGACCCAGCACGATCAACATCAGCGCGACGACAAAACCGa                 <  1:3613647/70‑1 (MQ=255)

                                                 tgttgtACAAAACCGCTTACTGCCGGGAACAGACCCAGCACGATCAACATCAGCGCGACGACAAAACCGa                 >  1:3429629/1‑70 (MQ=255)

                                                   ttgtACAAAACCGCTTACTGCCGGGAACAGACCCAGCACGATCAACATCAGCGCGACGACAAAACCGa                 <  1:2025868/68‑1 (MQ=255)

                                                   ttgtACAAAACCGCTTACTGCCGGGAACAGACCCAGCACGATCAACATCAGCGCGACGACAAAACCGa                 <  1:2848896/68‑1 (MQ=255)

                                                        cAAAACCGCTTACTGCCGGGAACAGACCCAGCACGATCAACATCAGCGCGACGACAAAAcc                   >  1:291655/1‑61 (MQ=255)

                                                        cAAAACCGCTTACTGCCGGGAACAGACCCAGCACGATCAACATCAGCGCGACGACAAAACCGACATAGCgg         >  1:2379880/1‑71 (MQ=255)

                                                        cAAAACCGCTTACTGCCGGGAACAGACCCAGCACGATCAACATCAGCGCGACGACAAAACCGACATAGCgg         >  1:1037657/1‑71 (MQ=255)

                                                        cAAAACCGCTTACTGCCGGGAACAGACCCAGCACGATCAACATCAGCGCGACGACAAAACCGACATAGCg          <  1:2473716/70‑1 (MQ=255)

                                                          aaaCCGCTTACTGCCGGGAACAGACCCAGCACGATCAACATCAGCGCGACGACAAAAcc                   <  1:2818665/59‑1 (MQ=255)

                                                            aCCGCTTACTGCCGGGAACAGACCCAGCACGATCAACATCAGCGCGACGACAAAACCGACATAGCGGCTgg     >  1:3232082/1‑71 (MQ=255)

                                                             ccGCTTACTGCCGGGAACAGACCCAGCACGATCAACATCAGCGCGACGACAAAACCGACATAGCGGCTGGc    <  1:2658771/71‑1 (MQ=255)

                                                             ccGCTTACTGCCGGGAACAGACCCAGCACGATCAACATCAGCGCGACGACAAAACCGACATAGCGGCTGGc    <  1:1610472/71‑1 (MQ=255)

                                                             ccGCTTACTGCCGGGAACAGACCCAGCACGATCAACATCAGCGCGACGACAAAACCGACATAGCGGCTGGc    <  1:1532713/71‑1 (MQ=255)

                                                              cGCTTACTGCCGGGAACAGACCCAGCACGATCAACATCAGCGCGACGACAAAACCGACATAGCGGCTGGc    <  1:1567107/70‑1 (MQ=255)

                                                               gCTTACTGCCGGGAACAGACCCAGCACGATCAACATCAGCGCGACGACAAAACCGACATAGCGGCTGGCaa  <  1:1678483/71‑1 (MQ=255)

GGTGCCAAACATTACAAGCGTTGCGCCGCCCAGAACCGGTTCTGGAATGTGTTGTACAAAACCGCTCACTGCCGGGAACAGACCCAGCACGATCAACATCAGCGCGACGACAAAACCGACATAGCGGCTGGCAA  >  W3110S.gb/3810303‑3810436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`