Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,918,430 G→A L264L (CTG→TTG)  insK ← IS150 conserved protein InsB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,918,4300GA100.0% 316.0 / NA 105L264L (CTG→TTG) insKIS150 conserved protein InsB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (59/46);  total (59/46)

Only 100 of 105 total aligned reads displayed.

GTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCATCCTTCAGTTCGCTTATATT  >  W3110S.gb/3918367‑3918496
                                                               |                                                                  
gTTAAACACGGGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTAAt                                                              >  1:4224429/1‑70 (MQ=255)
 ttAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCt                                                                 <  1:1510251/66‑1 (MQ=255)
 ttAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCt                                                                 <  1:2841314/66‑1 (MQ=255)
 ttAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTAAttc                                                            <  1:4357434/71‑1 (MQ=255)
  tAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTAAttct                                                           >  1:4070078/1‑71 (MQ=255)
  tAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTAAttct                                                           >  1:4447575/1‑71 (MQ=255)
  tAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTAAttc                                                            <  1:1607745/70‑1 (MQ=255)
   aaaCACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTaa                                                               >  1:3856699/1‑66 (MQ=255)
   aaaCACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTAAttct                                                           >  1:2655580/1‑70 (MQ=255)
     acacGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTAATtcttct                                                        <  1:1863339/71‑1 (MQ=255)
      cacGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTAAtt                                                             >  1:3647267/1‑65 (MQ=255)
      cacGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTAATtcttct                                                        >  1:134948/1‑70 (MQ=255)
            gCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTAATTCTTCTGCtgttg                                                 <  1:3020025/71‑1 (MQ=255)
               tataGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCt                                                                 <  1:629344/52‑1 (MQ=255)
               tataGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTaa                                                               <  1:1826551/54‑1 (MQ=255)
               tataGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTAAttc                                                            <  1:4605668/57‑1 (MQ=255)
               tataGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTgtag                                              <  1:4466989/71‑1 (MQ=255)
               tataGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTgtag                                              <  1:3080150/71‑1 (MQ=255)
                 taGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTaa                                                               >  1:954344/1‑52 (MQ=255)
                 taGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTaa                                                               >  1:1489858/1‑52 (MQ=255)
                 taGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTAAtt                                                             >  1:2135776/1‑54 (MQ=255)
                 taGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTgtagta                                            >  1:372465/1‑71 (MQ=255)
                 taGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTgtagta                                            >  1:4014608/1‑71 (MQ=255)
                 taGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTgtagta                                            >  1:3099650/1‑71 (MQ=255)
                 taGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTgtagta                                            >  1:4052744/1‑71 (MQ=255)
                 taGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTgtagta                                            >  1:538614/1‑71 (MQ=255)
                 taGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTgtagta                                            >  1:58763/1‑71 (MQ=255)
                 taGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTgtagta                                            >  1:952161/1‑71 (MQ=255)
                 taGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTgtagta                                            >  1:2902731/1‑71 (MQ=255)
                 taGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTgtagta                                            >  1:2618957/1‑71 (MQ=255)
                 taGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTgtagta                                            >  1:2500986/1‑71 (MQ=255)
                 taGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTgtagta                                            >  1:230144/1‑71 (MQ=255)
                 taGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTgtagta                                            >  1:2067164/1‑71 (MQ=255)
                 taGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTgtagta                                            >  1:206527/1‑71 (MQ=255)
                 taGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTgtagta                                            >  1:1959516/1‑71 (MQ=255)
                 taGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTgtagta                                            >  1:1577775/1‑71 (MQ=255)
                 taGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTgtagta                                            >  1:1169449/1‑71 (MQ=255)
                 taGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTgtagta                                            >  1:1362396/1‑71 (MQ=255)
                 taGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGATAATTCTTCTGCTGTTgtagta                                            >  1:1561152/1‑71 (MQ=255)
                 taGGTCTGATTCCGATATTAAATTGGAGTCAGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTgtagta                                            >  1:1712897/1‑71 (MQ=255)
                   ggTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTGTAGTAtt                                          >  1:1691298/1‑71 (MQ=255)
                    gTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTGTAGTATTc                                         <  1:1121400/71‑1 (MQ=255)
                      cTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTGTAGTATTCaa                                       >  1:2947890/1‑71 (MQ=255)
                       tGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTGTAGTATTCaa                                       >  1:3077908/1‑70 (MQ=255)
                         aTTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTaa                                                               >  1:3994593/1‑44 (MQ=255)
                         aTTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTaa                                                               >  1:1733745/1‑44 (MQ=255)
                         aTTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTgtagta                                            >  1:1949611/1‑63 (MQ=255)
                         aTTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTGTAGTATTCaa                                       <  1:2546111/68‑1 (MQ=255)
                         aTTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTGTAGTATTCAata                                     <  1:580203/70‑1 (MQ=255)
                         aTTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTGTAGTATTCAata                                     <  1:4124964/70‑1 (MQ=255)
                         aTTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTGTAGTATTCAata                                     <  1:1619790/70‑1 (MQ=255)
                         aTTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTGTAGTATTCAata                                     >  1:1895806/1‑70 (MQ=255)
                         aTTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTGTAGTATTCAata                                     <  1:1823630/70‑1 (MQ=255)
                          ttCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTGTAGTATTCAatata                                   <  1:197192/71‑1 (MQ=255)
                          ttCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTGTAGTATTCAatata                                   <  1:2235508/71‑1 (MQ=255)
                            ccGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTGTAGTAtt                                          >  1:1374651/1‑62 (MQ=255)
                            ccGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTGTAGTATTCAatatat                                  <  1:1909449/70‑1 (MQ=255)
                            ccGATATTCAATTGGAGTCAGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTGTAGTATTCAATATAtt                                 >  1:2546325/1‑71 (MQ=255)
                                tatTCAATTGGAGTCAGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGt                             <  1:2947039/71‑1 (MQ=255)
                                 atTCAATTGGAGTCAGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGt                             >  1:785260/1‑70 (MQ=255)
                                  ttCAATTGGAGTCAGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGt                             <  1:4675653/69‑1 (MQ=255)
                                   tCAATTGGAGTCTGACCTTTTAATTTCAAGCTAATTCTTCTGCtgt                                                   <  1:3581615/46‑1 (MQ=39)
                                    cAATTGGAGTCAGACCTTTTAATTTCAAGCTAATTCTTctg                                                       >  1:2585207/1‑41 (MQ=255)
                                    cAATTGGAGTCAGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTaa                           >  1:3661281/1‑69 (MQ=255)
                                    cAATTGGAGTCAGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAAc                          <  1:4602089/70‑1 (MQ=255)
                                       ttGGAGTCAGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCa                      <  1:3416242/71‑1 (MQ=255)
                                       ttGGAGTCAGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCa                      >  1:2397585/1‑71 (MQ=255)
                                         ggAGTCAGACCTTTTAATTTCAAGCTAATTCTTctgct                                                     >  1:85341/1‑38 (MQ=255)
                                         ggAGTCAGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTaa                           >  1:2954661/1‑64 (MQ=255)
                                         ggAGTCAGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCAt                     <  1:2804933/70‑1 (MQ=255)
                                             tCAGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTGTAGTATTCAata                                     <  1:4151789/50‑1 (MQ=255)
                                             tCAGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTGTAGTATTCAata                                     <  1:3993972/50‑1 (MQ=255)
                                             tCAGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTGTAGTATTCAata                                     <  1:3154881/50‑1 (MQ=255)
                                             tCAGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCATCCTTc                <  1:944153/71‑1 (MQ=255)
                                               aGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTgtag                                              >  1:4550033/1‑39 (MQ=255)
                                               aGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCATcc                   >  1:924014/1‑66 (MQ=255)
                                               aGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCATCCTTCa               >  1:4515554/1‑70 (MQ=255)
                                               aGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCATCCTTCAg              >  1:3880225/1‑71 (MQ=255)
                                               aGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCATCCTTCAg              >  1:222925/1‑71 (MQ=255)
                                               aGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCATCCTTCAg              >  1:1203689/1‑71 (MQ=255)
                                               aGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCATCCTTCAg              >  1:1331677/1‑71 (MQ=255)
                                               aGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCATCCTTCAg              >  1:3185105/1‑71 (MQ=255)
                                               aGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCATCCTTCAg              >  1:2978778/1‑71 (MQ=255)
                                               aGACCTTTTAATTTCAAGCTAATTCTTCTGCTGTTCTAGTATTCAATATATTCCGTAACAGCATCCTTCAg              >  1:1653720/1‑71 (MQ=255)
                                               aGAACTTTTAATTTCAAGATAATTCTTCTGCTGTTgtag                                              >  1:4630680/1‑39 (MQ=25)
                                                 aCCTTTTAATTTCAAGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCATCCTTCAGtt            >  1:3591908/1‑71 (MQ=255)
                                                   cTTTTAATTTCAAGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCATCCTTCAGTTc           >  1:1720745/1‑70 (MQ=255)
                                                      ttAATTTCAAGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCATCCTTCAGTTCGCt        <  1:1490348/70‑1 (MQ=255)
                                                      ttAATTTCAAGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCATCCTTCAGTTCGCt        <  1:1258732/70‑1 (MQ=255)
                                                        aaTTTCAAGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCATCCTTCAGTTCGCTta      <  1:3605097/70‑1 (MQ=255)
                                                        aaTTTCAAGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCATCCTTCAGTTCGCTta      >  1:1901506/1‑70 (MQ=255)
                                                        aaTTTCAAGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCATCCTTCAGTTCGCTta      <  1:1853814/70‑1 (MQ=255)
                                                        aaTTTCAAGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCATCCTTCAGTTCGCTta      <  1:1025247/70‑1 (MQ=255)
                                                        aaTTTCAAGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCATCCTTCAGTTCGCTta      <  1:861322/70‑1 (MQ=255)
                                                         aTTTCAAGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTTACAGCATCCTTCAGTTCGCTtat     <  1:2218670/70‑1 (MQ=255)
                                                         aTTTCAAGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCATCCTTCAGTTCGCTta      <  1:1112923/69‑1 (MQ=255)
                                                         aTTTCAAGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCATCCTTCAGTTCGCTta      <  1:3090642/69‑1 (MQ=255)
                                                         aTTTCAAGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCATCCTTCAGTTCGCTta      <  1:2872553/69‑1 (MQ=255)
                                                            tCAAGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCATCCTTCAGTTCGCTTATAtt  <  1:4675208/70‑1 (MQ=255)
                                                            tCAAGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCATCCTTCAGTTCGCTTATAtt  <  1:1383085/70‑1 (MQ=255)
                                                               |                                                                  
GTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCATCCTTCAGTTCGCTTATATT  >  W3110S.gb/3918367‑3918496

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑