Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,025,948	A→G	I268T (ATC→ACC)	nikA ←	nickel transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,025,948	0	A	G	100.0%	148.1 / NA	43	I268T (ATC→ACC)	nikA	nickel transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/21);  total (22/21)

CTTCACGTACTGCCAGCTCGTTGGTGGGGGCTTTGGCGGTATTGAGCGCCAGCATCACGGTTTCGATCGGCTGTGACAGTTGGGTGTGGTAAGCCGGATTCTGGCTAAAGCGGGCGAAGGTATCGAGCGGTAA  >  W3110S.gb/4025883‑4026015                                                                  |                                                                    cTTCACGTACTGCCAGCTCGTTGGTGGGGGCTTTGGCGGTATTGAGCGCCAGCATCACGGTTTCGGTCGGc                                                                <  1:1066748/71‑1 (MQ=255) cTTCACGTACTGCCAGCTCGTTGGTGGGGGCTTTGGCGGTATTGAGCGCCAGCATCACGGTTTCGGTCGGc                                                                <  1:2242017/71‑1 (MQ=255) cTTCACGTACTGCCAGCTCGTTGGTGGGGGCTTTGGCGGTATTGAGCGCCAGCATCACGGTTTCGGTCGGc                                                                <  1:675061/71‑1 (MQ=255)    cACGTACTGCCAGCTCGTTGGTGGGGGCTTTGGCGGTATTGAGCGCCAGCATCACGGTTTCGGTCGGCtgt                                                             >  1:4349196/1‑71 (MQ=255)    cACGTACTGCCAGCTCGTTGGTGGGGGCTTTGGCGGTATTGAGCGCCAGCATCACGGTTTCGGTCGGCtgt                                                             >  1:1615401/1‑71 (MQ=255)    cACGTACTGCCAGCTCGTTGGTGGGGGCTTTGGCGGTATTGAGCGCCAGCATCACGGTTTCGGTCGGCtg                                                              >  1:654470/1‑70 (MQ=255)       gTACTGCCAGCTCGTTGGTGGGGGCTTTGGCGGTATTGAGCGCCAGCATCACGGTTTCGGTCGGCTGTGAc                                                          <  1:2029475/71‑1 (MQ=255)        tACTGCCAGCTCGTTGGTGGGGGCTTTGGCGGTATTGAGCGCCAGCATCACGGTTTCGGTCGGCTGTGACa                                                         <  1:942831/71‑1 (MQ=255)        tACTGCCAGCTCGTTGGTGGGGGCTTTGGCGGTATTGAGCGCCAGCATCACGGTTTCGGTCGGCTGTGACa                                                         <  1:420493/71‑1 (MQ=255)        tACTGCCAGCTCGTTGGTGGGGGCTTTGGCGGTATTGAGCGCCAGCATCACGGTTTCGGTCGGCTGTGACa                                                         <  1:1905224/71‑1 (MQ=255)              cAGCTCGTTGGTGGGGGCTTTGGCGGTATTGAGCGCCAGCATCACGGTTTCGGTCGGCTGTGACAGTTggg                                                   <  1:3843269/71‑1 (MQ=255)              cAGCTCGTTGGTGGGGGCTTTGGCGGTATTGAGCGCCAGCATCACGGTTTCGGTCGGCTGTGACAGTTggg                                                   <  1:3659021/71‑1 (MQ=255)               aGCTCGTTGGTGGGGGCTTTGGCGGTATTGAGCGCCAGCATCACGGTTTCGGTCGGCTGTGACAGTTGGgt                                                  >  1:4657681/1‑71 (MQ=255)                 cTCGTTGGTGGGGGCTTTGGCGGTATTGAGCGCCAGCATCACGGTTTCGGTCGGCTGTGACAGTTGGgtg                                                 >  1:3353777/1‑70 (MQ=255)                    gTTGGTGGGGGCTTTGGCGGTATTGAGCGCCAGCATCACGGTTTCGGTCGGCTGTGACAGTTGGGTGTGGt                                             <  1:207580/71‑1 (MQ=255)                    gTTGGTGGGGGCTTTGGCGGTATTGAGCGCCAGCATCACGGTTTCGGTCGGCTGTGACAGTTGGGTGTGGt                                             <  1:2608426/71‑1 (MQ=255)                     ttGGTGGGGGCTTTGGCGGTATTGAGCGCCAGCATCACGGTTTCGGTCGGCtgt                                                             <  1:584227/54‑1 (MQ=255)                       ggtggGGGCTTTGGCGGTATTGAGCGCCAGCATCACGGTTTCGGTCGGCTGTGACAGTTGGGTGTGGTAAg                                          >  1:4545423/1‑71 (MQ=255)                         tggGGGCTTTGGCGGTATTGAGCGCCAGCATCACGGTTTCGGTCGGCtgt                                                             <  1:1862647/50‑1 (MQ=255)                                  tGGCGGTATTGAGCGCCAGCATCACGGTTTCGGTCGGCTGTGACAGTTGGGTGTGGTAAGCCGGATTCt                                 <  1:4336653/69‑1 (MQ=255)                                       gTATTGAGCGCCAGCATCACGGTTTCGGTCGGCTGTGACAGTTGGGTGTGGTAAGCCGGATTCTGGCTaaa                          <  1:348224/71‑1 (MQ=255)                                         aTTGAGCGCCAGCATCACGGTTTCGGTCGGCTGTGACAGTTGGGTGTGGTAAGCCGGATTCTGGCTAAAGc                        >  1:2876008/1‑71 (MQ=255)                                            gAGCGCCAGCATCACGGTTTCGGTCGGCTGTGACAGTTGGGTGTGGTAAGCCGGATTCTGGCTAAAGCggg                     >  1:4167826/1‑71 (MQ=255)                                            gAGCGCCAGCATCACGGTTTCGGTCGGCTGTGACAGTTGGGTGTGGTAAGCCGGATTCTGGCTAAAGCggg                     >  1:783264/1‑71 (MQ=255)                                            gAGCGCCAGCATCACGGTTTCGGTCGGCTGTGACAGTTGGGTGTGGTAAGCCGGATTCTGGCTAAAGCgg                      >  1:1208286/1‑70 (MQ=255)                                                  cAGCATCACGGTTTCGGTCGGCTGTGACAGTTGGGTGTGGTAAGCCGGATTCTGGCTAAAGCGGGCGAAg                <  1:2027423/70‑1 (MQ=255)                                                      aTCACGGTTTCGGTCGGCTGTGACAGTTGGGTGTGGTAAGCCGGATTCTGGCTAAAGCGGGCGAAGGTa             >  1:1063102/1‑69 (MQ=255)                                                       tCACGGTTTCGGTCGGCTGTGACAGTTGGGTGTGGTaa                                           <  1:869348/38‑1 (MQ=255)                                                       tCACGGTTTCGGTCGGCTGTGACAGTTGGGTGTGGTAAGCCGGAt                                    <  1:917920/45‑1 (MQ=255)                                                         aCGGTTTCGGTCGGCTGTGAGAGTTGGGTGTGGTAAGCCGGATTCTGGCTAAAGCGGGCGAAGGTATCGAg        >  1:3039455/1‑71 (MQ=255)                                                         aCGGTTTCGGTCGGCTGTGACAGTTGGGTGTGGTAAGc                                         >  1:3661107/1‑38 (MQ=255)                                                         aCGGTTTCGGTCGGCTGTGACAGTTGGGTGTGGTAAGc                                         >  1:2697684/1‑38 (MQ=255)                                                         aCGGTTTCGGTCGGCTGTGACAGTTGGGTGTGGTAAGc                                         >  1:1711566/1‑38 (MQ=255)                                                         aCGGTTTCGGTCGGCTGTGACAGTTGGGTGTGGTAAGCCGGATTCTGGCTAAAGCGGGCGAAGGTAt            >  1:3167294/1‑67 (MQ=255)                                                         aCGGTTTCGGTCGGCTGTGACAGTTGGGTGTGGTAAGCCGGATTCTGGCTAAAGCGGGCGAAGGTATCGAg        >  1:1748414/1‑71 (MQ=255)                                                         aCGGTTTCGGTCGGCTGTGACAGTTGGGTGTGGTAAGCCGGATTCTGGCTAAAGCGGGCGAAGGTATCGAg        >  1:1571695/1‑71 (MQ=255)                                                         aCGGTTTCGGTCGGCTGTGACAGTTGGGTGTGGTAAGCCGGATTCTGGCTAAAGCGGGCGAAGGTATCGAg        >  1:1600493/1‑71 (MQ=255)                                                         aCGGTTTCGGTCGGCTGTGACAGTTGGGTGTGGTAAGCCGGATTCTGGCTAAAGCGGGCGAAGGTATCGAg        >  1:1698006/1‑71 (MQ=255)                                                         aCGGTTTCGGTCGGCTGTGACAGTTGGGTGTGGTAAGCCGGATTCTGGCTAAAGCGGGCGAAGGTATCGAg        >  1:2552234/1‑71 (MQ=255)                                                         aCGGTTTCGGTCGGCTGTGACAGTTGGGTGTGGTAAGCCGGATTCTGGCTAAAGCGGGCGAAGGTATCGAg        >  1:2899486/1‑71 (MQ=255)                                                             tttCGGTCGGCTGTGACAGTTGGGTGTGGTAAGCCGGATTCTGGCTAAAGCGGGCg                   <  1:1046903/56‑1 (MQ=255)                                                             tttCGGTCGGCTGTGACAGTTGGGTGTGGTAAGCCGGATTCTGGCTAAAGCGGGCGAAGGTATCGAGCGGt    <  1:4207066/71‑1 (MQ=255)                                                             tttCGGTCGGCTGTGACAGTTGGGTGTGGTAAGCCGGATTCTGGCTAAAGCGGGCGAAGGTATCGAGCGGt    <  1:134339/71‑1 (MQ=255)                                                               tcggtcggCTGTGACAGTTGGGTGTGGTAAGCCGGATTCTGGCTAAAGCGGGCGAAgg               >  1:3245538/1‑58 (MQ=255)                                                               tcggtcggCTGTGACAGTTGGGTGTGGTAAGCCGGATTCTGGCTAAAGCGGGCGAAGGTATCGAGCGGtaa  >  1:3819948/1‑71 (MQ=255)                                                                  |                                                                    CTTCACGTACTGCCAGCTCGTTGGTGGGGGCTTTGGCGGTATTGAGCGCCAGCATCACGGTTTCGATCGGCTGTGACAGTTGGGTGTGGTAAGCCGGATTCTGGCTAAAGCGGGCGAAGGTATCGAGCGGTAA  >  W3110S.gb/4025883‑4026015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑