Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 4,031,980 T→C N663D (AAC→GAC)  zntA ← zinc, cobalt and lead efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,031,9800TC100.0% 177.6 / NA 52N663D (AAC→GAC) zntAzinc, cobalt and lead efflux system
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/30);  total (22/30)

GTTCTGGCGGATATTGGCGTGAGTGGCGCGTGCCAGTTCAATCATTTGCACCAGGCCGCGCAGGTGGTTATGGGTTAATGCTGCGTCGGCGGTTTCCAGCGCCACGTCTGTGCCGCTACCCATTGCAATCCCGATG  >  W3110S.gb/4031912‑4032047
                                                                    |                                                                   
gTTCTGGCGGATATTGGCGTGAGTGGCGCGTGCCAGTTCAATCATTTGCACCAGGCCGCGCAGGTGGTCAt                                                                   >  1:3545026/1‑71 (MQ=255)
  tCTGGCGGATATTGGCGTGAGTGGCGCGTGCCAGTTCAATCATTTGCACCAGGCCGCGCAGGTGGTCATgg                                                                 >  1:3194602/1‑71 (MQ=255)
  tCTGGCGGATATTGGCGTGAGTGGCGCGTGCCAGTTCAATCATTTGCACCAGGCCGCGCAGGTGGTCATgg                                                                 >  1:1145622/1‑71 (MQ=255)
      gCGGATATTGGCGTGAGTGGCGCGTGCCAGTTCAATCATTTGCACCAGGCCGCGCAGGTGGTCATGTGtt                                                              <  1:2274143/70‑1 (MQ=255)
      gCGGATATTGGCGTGAGTGGCGCGTGCCAGTTCAATCATTTGCACCAGGCCGCGCAGGTGGTCATGGGtt                                                              <  1:961362/70‑1 (MQ=255)
      gCGGATATTGGCGTGAGTGGCGCGTGCCAGTTCAATCATTTGCACCAGGCCGCGCAGGTGGTCATGGGtt                                                              <  1:2759620/70‑1 (MQ=255)
      gCGGATATTGGCGTGAGTGGCGCGTGCCAGTTCAATCATTTGCACCAGGCCGCGCAGGTGGTCATGGGtt                                                              <  1:2733907/70‑1 (MQ=255)
        ggATATTGGCGTGAGTGGCGCGTGCCAGTTCAATCATTTGCACCAGGCCGCGCAGGTGGTCATGGGTTAAt                                                           <  1:3878172/71‑1 (MQ=255)
         gATATTGGCGTGAGTGGCGCGTGCCAGTTCAATCATTTGCACCAGGCCGCGCAGGTGGTCATGGGtt                                                              <  1:2491372/67‑1 (MQ=255)
             ttGGCGTGAGTGGCGCGTGCCAGTTCAATCATTTGCACCAGGCCGCGCAGGTGGTCATGGGTTaa                                                            >  1:2424722/1‑65 (MQ=255)
             ttGGCGTGAGTGGCGCGTGCCAGTTCAATCATTTGCACCAGGCCGCGCAGGTGGTCATGGGTTAAtgctgc                                                      >  1:3289018/1‑71 (MQ=255)
                     aGTGGCGCGTGCCAGTTCAATCATTTGCACCAGGCCGCGCAGGTGGTCATGGGTTAATGCTGCGTcggcgg                                              >  1:130864/1‑71 (MQ=255)
                         gcgcGTGCCAGTTCAATCATTTGCACCAGGCCGCGCAGGTGGTCATGGGTTAATGCTGCGTCGGCGGTTTc                                          >  1:590576/1‑71 (MQ=255)
                             gTGCCAGTTCAATCATTTGCACCAGGCCGCGCAGGTGGTCATGGGTTAATGCTGCGTCGGCGGTTTCCAgc                                      <  1:2347982/71‑1 (MQ=255)
                                ccAGTTCAATCATTTGCACCAGGCCGCGCAGGTGGTCATGGGtt                                                              <  1:292684/44‑1 (MQ=255)
                                  aGTTCAATCATTTGCACCAGGCCGCGCAGGTGGTCATGGGTTaa                                                            >  1:4266755/1‑44 (MQ=255)
                                   gTTCAATCATTTGCACCAGGCCGCGCAGGTGGTCATGGGTTAATGCTGCGTCGGCGGTTTCCAGCGCCACg                                <  1:4331940/71‑1 (MQ=255)
                                     tCAATCATTTGCACCAGGCCGCGCAGGTGGTCATGGGTTAATGCTGCGTCGGCGGTTTCCAGCGCCACGTc                              >  1:4469590/1‑71 (MQ=255)
                                     tCAATCATTTGCACCAGGCCGCGCAGGTGGTCATGGGTTAATGCTGCGTCGGCGGTTTCCAGCGCCACGTc                              >  1:84138/1‑71 (MQ=255)
                                     tCAATCATTTGCACCAGGCCGCGCAGGTGGTCATGGGTTAATGCTGCGTCGGCGGTTTCCAGCGCCACGTc                              >  1:3713362/1‑71 (MQ=255)
                                      cAATCATTTGCACCAGGCCGCGCAGGTGGTCATGGGTTAATGCTGCGTCGGCGGTTTCCAGCGCCACGTc                              <  1:2170969/70‑1 (MQ=255)
                                      cAATCATTTGCACCAGGCCGCGCAGGTGGTCATGGGTTAATGCTGCGTCGGCGGTTTCCAGCGCCACGTCt                             >  1:682428/1‑71 (MQ=255)
                                      cAATCATTTGCACCAGGCCGCGCAGGTGGTCATGGGTTAATGCTGCGTCGGCGGTTTCCAGCGCCACGTCt                             >  1:4229121/1‑71 (MQ=255)
                                       aaTCATTTGCACCAGGCCGCGCAGGTGGTCATGGGTTAATGCTGCGTCGGCGGTTTCCAGCGCCACGTc                              <  1:896458/69‑1 (MQ=255)
                                       aaTCATTTGCACCAGGCCGCGCAGGTGGTCATGGGTTAATGCTGCGTCGGCGGTTTCCAGCGCCACGTCtg                            <  1:2608932/71‑1 (MQ=255)
                                          cATTTGCACCAGGCCGCGCAGGTGGTCATGGGTTAATGCTGCGTCGGCGGTTTCCAGCGCCACGTCTGTGc                         >  1:3340471/1‑71 (MQ=255)
                                          cATTTGCACCAGGCCGCGCAGGTGGTCATGGGTTAATGCTGCGTCGGCGGTTTCCAGCGCCACGTCTGTGc                         >  1:2069704/1‑71 (MQ=255)
                                            tttGCACCAGGCCGCGCAGGTGGTCATGGGTTAATGCTGCGTCGGCGGTTTCCAGCGCCACGTc                              <  1:2102368/64‑1 (MQ=255)
                                              tGCACCAGGCCGCGCAGGTGGTCATGGGTTAATGCTGCGTCGGCGGTTTCCAGCGCCACGTCTGTGCCg                       >  1:3047918/1‑69 (MQ=255)
                                              tGCACCAGGCCGCGCAGGTGGTCATGGGTTAATGCTGCGTCGGCGGTTTCCAGCGCCACGTCTGTGCCGc                      >  1:1085316/1‑70 (MQ=255)
                                                 aCCAGGCCGCGCAGGTGGTCATGGGTTAATGCTGCGTCGGCGGTTTCCAGCGCCACGTCTGTGCCGCTAcc                  <  1:112347/71‑1 (MQ=255)
                                                 aCCAGGCCGCGCAGGTGGTCATGGGTTAATGCTGCGTCGGCGGTTTCCAGCGCCACGTCTGTGCCGCTAcc                  <  1:3761024/71‑1 (MQ=255)
                                                 aCCAGGCCGCGCAGGTGGTCATGGGTTAATGCTGCGTCGGCGGTTTCCAGCGCCACGTCTGTGCCGCTAcc                  <  1:620994/71‑1 (MQ=255)
                                                 aCCAGGCCGCGCAGGTGGTCATGGGTTAATGCTGCGGCGGCGGTTTCCAGCGCCACGTCTGTGCCGCTAcc                  <  1:713391/71‑1 (MQ=255)
                                                  ccAGGCCGCGCAGGTGGTCATGGGTTAATGCTGCGTCGGCGGTTTCCAGCGCCACGTCTGTGCCGCTAcc                  >  1:1863216/1‑70 (MQ=255)
                                                   cAGGCCGCGCAGGTGGTCATGGGTTAATGCTGCGTCGGCGGTTTCCAGCGCCACGTCTGTGCCGCTACCCa                <  1:1720463/71‑1 (MQ=255)
                                                     ggCCGCGCAGGTGGTCATGGGTTAATGCTGCGTCGGCGGTTTCCAGCGCCACGTCTGTGCCGCTACCCAtt              >  1:956862/1‑71 (MQ=255)
                                                       ccGCGCAGGTGGTCATGGGTTAATGCTGCGTCGGCGGTTTCCAGCGCCACGTCTGTGCCGCTACCCATTGc            <  1:1086693/71‑1 (MQ=255)
                                                       ccGCGCAGGTGGTCATGGGTTAATGCTGCGTCGGCGGTTTCCAGCGCCACGTCTGTGCCGCTACCCATTGc            <  1:3159848/71‑1 (MQ=255)
                                                       ccGCGCAGGTGGTCATGGGTTAATGCTGCGTCGGCGGTTTCCAGCGCCACGTCTGTGCCGCTACCCATTGc            <  1:2211728/71‑1 (MQ=255)
                                                            cAGGTGGTCATGGGTTAATGCTGCGTCGGCGGTTTCCAGCGCCACGTCTGTGCCGCTACCCa                >  1:2611052/1‑62 (MQ=255)
                                                             aGGTGGTCATGGGTTAATGCTGCGTCGGCGGTTTCCAGCGCCACGTCTGTGCCGCTACCCATTGc            <  1:2169931/65‑1 (MQ=255)
                                                             aGGTGGTCATGGGTTAATGCTGCGTCGGCGGTTTCCAGCGCCACGTCTGTGCCGCTACCCATTGc            <  1:197598/65‑1 (MQ=255)
                                                             aGGTGGTCATGGGTTAATGCTGCGTCGGCGGTTTCCAGCGCCACGTCTGTGCCGCTACCCATTGc            <  1:2590951/65‑1 (MQ=255)
                                                             aGGTGGTCATGGGTTAATGCTGCGTCGGCGGTTTCCAGCGCCACGTCTGTGCCGCTACCCATTGCAATccc      <  1:4116288/71‑1 (MQ=255)
                                                             aGGTGGTCATGGGTTAATGCTGCGTCGGCGGTTTCCAGCGCCACGTCTGTGCCGCTACCCATTGCAATccc      <  1:1389503/71‑1 (MQ=255)
                                                             aGGTGGTCATGGGTTAATGCTGCGTCGGCGGTTTCCAGCGCCACGTCTGTGCCGCTACCCATTGCAATccc      <  1:2636308/71‑1 (MQ=255)
                                                             aGGTGGTCATGGGTTAATGCTGCGTCGGCGGTTTCCAGCGCCACGTCTGTGCCGCTACCCATTGCAATccc      <  1:92172/71‑1 (MQ=255)
                                                             aGGTGGTCATGGGTTAATGCTGCGTCGGCGGTTTCCAGCGCCACGTCTGTGCCGCTACCCATTGCAATccc      <  1:3498521/71‑1 (MQ=255)
                                                             aGGTGGTCATGGGTTAATGCTGCGTCGGCGGTTTCCAGCGCCACGTCTGTGCCGCTACCCATTGCAATccc      <  1:3358407/71‑1 (MQ=255)
                                                                tggtCATGGGTTAATGCTGCGTCGGCGGTTTCCAGCGCCACGTCTGTGCCGCTACCCATTGCAATCCCGAt   >  1:3965122/1‑71 (MQ=255)
                                                                 ggtCATGGGTTAATGCTGCGTCGGCGGTTTCCAGCGCCACGTCTGTGCCGCTACCCATTGCAATCCCGATg  >  1:1154430/1‑71 (MQ=255)
                                                                    |                                                                   
GTTCTGGCGGATATTGGCGTGAGTGGCGCGTGCCAGTTCAATCATTTGCACCAGGCCGCGCAGGTGGTTATGGGTTAATGCTGCGTCGGCGGTTTCCAGCGCCACGTCTGTGCCGCTACCCATTGCAATCCCGATG  >  W3110S.gb/4031912‑4032047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑