Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,042,971	G→A	A39T (GCC→ACC)	livK →	leucine transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,042,971	0	G	A	100.0%	167.7 / NA	56	A39T (GCC→ACC)	livK	leucine transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/36);  total (20/36)

CAATTTCACACACCGCTATGGCTGACGATATTAAAGTCGCCGTTGTCGGCGCGATGTCCGGCCCGATTGCCCAGT‑GGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGACATTAATGCCAAAGGGGG  >  W3110S.gb/4042903‑4043038                                                                     |                                                                     cAATTTCACACACCGCTATGGCTGACGATATCAAAGTCGCCGTTGTCGGCGCGATGTCCGGCCCGATTAcc                                                                    <  1:203908/71‑1 (MQ=255)  aaTTTCACACACCGCTATGGCTGACGATATTAAAGTCGCCGTTGTCGGCGCGATGTCCGGCCCGATTAcc                                                                    >  1:3230951/1‑70 (MQ=255)     ttCACACACCGCTATGGCTGACGATATTAAAGTCGCCGTTGTCGGCGCGATGTCCGGCCCGATTACCCAGt                                                                >  1:4103775/1‑71 (MQ=255)     ttCACACACCGCTATGGCTGACGATATTAAAGTCGCCGTTGTCGGCGCGATGTCCGGCCCGATTACCCAGt                                                                >  1:332868/1‑71 (MQ=255)     ttCACACACCGCTATGGCTGAAGATATTAAAGTCGCCGTTGTCGGCGCGATGTCCGGCCCGATTACCCAGt                                                                >  1:2578787/1‑71 (MQ=255)      tCACACACCGCTATGGCTGACGATATTAAAGTCGCCGTTGTCGGCGCGATGTCCGGCCCGATTACCCAGT‑g                                                              >  1:1639838/1‑71 (MQ=255)            acCGCTATGGCTGACGATATTAAAGTCGCCGTTGTCGGCGCGATGTCCGGCCCGATTACCCAGT‑GGGGCGa                                                        <  1:963746/71‑1 (MQ=255)            acCGCTATGGCTGACGATATTAAAGTCGCCGTTGTCGGCGCGATGTCCGGCCCGATTACCCAGT‑GGGGCGa                                                        <  1:1349388/71‑1 (MQ=255)               gCTATGGGTGACGATATTAAAGTCGCCGTTGTCGGCGCGATGTCCGGCCCGATTACCCAGT‑GGGGCGatat                                                     >  1:3132201/1‑71 (MQ=255)               gCTATGGCTGACGATATTAAAGTCGCCGTTGTCGGCGCGATGTCCGGCCCGATTACCCAGT‑GGGGCGatat                                                     >  1:4636213/1‑71 (MQ=255)               gCTATGGCTGACGATATTAAAGTCGCCGTTGTCGGCGCGATGTCCGGCCCGATTACCCAGT‑GGGGCGatat                                                     >  1:420192/1‑71 (MQ=255)                   tGGCTGACGATATTAAAGTCGCCGTTGTCGGCGCGATGTCCGGCCCGATTACCCAGT‑GGGGCGATATgg                                                   <  1:672278/69‑1 (MQ=255)                      cTGACGATATTAAAGTCGCCGTTGTCGGCGCGATGTCCGGCCCGATTACCCAGT‑GGGGCGATATGGAAttt                                              <  1:1165608/71‑1 (MQ=255)                      cTGACGATATTAAAGTCGCCGTTGTCGGCGCGATGTCCGGCCCGATTACCCAGT‑GGGGCGATATGGAAttt                                              <  1:3740231/71‑1 (MQ=255)                      cTGACGATATTAAAGTCGCCGTTGTCGGCGCGATGTCCGGCCCGATTACCCAGT‑GGGGCGATATGGAAttt                                              <  1:3250637/71‑1 (MQ=255)                      cTGACGATATTAAAGTCGCCGTTGTCGGCGCGATGTCCGGCCCGATTACCCAGT‑GGGGCGATATGGAAttt                                              <  1:2794201/71‑1 (MQ=255)                         aCGATATTAAAGTCGCCGTTGTCGGCGCGATGTCCGGCCCGATTACCCAGT‑GGGGCGa                                                        <  1:2985939/58‑1 (MQ=255)                           gATATTAAAGTCGCCGTTGTCGGCGCGATGTCCGGCCCGATTACCCAGT‑GGGGCGATATGGAATTTAACgg                                         >  1:681991/1‑71 (MQ=255)                               ttAAAGTCGCCGTTGTCGGCGCGATGTCCGGCCCGATTACCCa                                                                  >  1:484390/1‑43 (MQ=255)                               ttAAAGTCGCCGTTGTCGGCGCGATGTCCGGCCCGATTACCCa                                                                  >  1:387904/1‑43 (MQ=255)                               ttAAAGTCGCCGTTGTCGGCGCGATGTCCGGCCCGATTACCCa                                                                  >  1:4267753/1‑43 (MQ=255)                               ttAAAGTCGCCGTTGTCGGCGCGATGTCCGGCCCGATTACCCAGT‑GGGGCGATATGGAAttt                                              <  1:2816265/62‑1 (MQ=255)                               ttAAAGTCGCCGTTGTCGGCGCGATGTCCGGCCCGATTACCCAGT‑GGGGCGATATGGAAttt                                              <  1:2755764/62‑1 (MQ=255)                                 aaaGTCGCCGTTGTCGGCGCGATGTCCGGCCCGATTACCCAGT‑GGGGCGATATGGAATTTAACGgcgcgc                                    >  1:589047/1‑70 (MQ=255)                                 aaaGTCGCCGTTGTCGGCGCGATGTCCGGCCCGATTACCCAGT‑GGGGCGATATGGAATTTAACGGcgcgcg                                   >  1:3926589/1‑71 (MQ=255)                                     tCGCCGTTGTCGGCGCGATGTCCGGCCCGATTACCCAGTGGGGGCGATATGGAATTTAACGGCGCGCGTCa                                <  1:1511785/71‑1 (MQ=255)                                      cGCCGTTGTCGGCGCGATGTCCGGCCCGATTACCCAGT‑GGGGCGa                                                        <  1:1202791/45‑1 (MQ=255)                                             gTCGGCGCGATGTCCGGCCCGATTACCCAGT‑GGGGCGata                                                      >  1:3299360/1‑40 (MQ=255)                                             gTCGGCGCGATGTCCGGCCCGATTACCCAGT‑GGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTa                        <  1:3458962/70‑1 (MQ=255)                                             gTCGGCGCGATGTCCGGCCCGATTACCCAGT‑GGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTa                        <  1:842843/70‑1 (MQ=255)                                             gTCGGCGCGATGTCCGGCCCGATTACCCAGT‑GGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTa                        <  1:65385/70‑1 (MQ=255)                                             gTCGGCGCGATGTCCGGCCCGATTACCCAGT‑GGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTa                        <  1:4601905/70‑1 (MQ=255)                                              tCGGCGCGATGTCCGGCCCGATTACCCAGT‑GGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTaaa                      <  1:3635904/71‑1 (MQ=255)                                                ggCGCGATGTCCGGCCCGATTACCCAGT‑GGGGCGATATGGAATTTaa                                            >  1:3905366/1‑47 (MQ=255)                                                ggCGCGATGTCCGGCCCGATTACCCAGT‑GGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGa                    <  1:3915564/71‑1 (MQ=255)                                                ggCGCGATGTCCGGCCCGATTACCCAGT‑GGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGa                    <  1:3800433/71‑1 (MQ=255)                                                ggCGCGATGTCCGGCCCGATTACCCAGT‑GGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGa                    <  1:946424/71‑1 (MQ=255)                                                ggCGCGATGTCCGGCCCGATTACCCAGT‑GGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGa                    >  1:3273538/1‑71 (MQ=255)                                                ggCGCGATGTCCGGCCCGATTACCCAGT‑GGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGa                    <  1:1385935/71‑1 (MQ=255)                                                ggCGCGATGTCCGGCCCGATTACCCAGT‑GGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGa                    <  1:3099448/71‑1 (MQ=255)                                                   gcgATGTCCGGCCCGATTACCCAGT‑GGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGAc                   >  1:4267065/1‑69 (MQ=255)                                                    cgATGTCCGGCCCGATTACCCAGT‑GGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGACAtt                <  1:2295641/71‑1 (MQ=255)                                                    cgATGTCCGGCCCGATTACCCAGT‑GGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGACAtt                <  1:4177642/71‑1 (MQ=255)                                                    cgATGTCCGGCCCGATTACCCAGT‑GGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGACAtt                <  1:450631/71‑1 (MQ=255)                                                    cgATGTCCGGCCCGATTACCCAGT‑GGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGACAtt                <  1:4660280/71‑1 (MQ=255)                                                    cgATGTCCGGCCCGATTACCCAGT‑GGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGACAtt                <  1:4668983/71‑1 (MQ=255)                                                    cgATGTCCGGCCCGATTACCCAGT‑GGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGACAtt                <  1:2938254/71‑1 (MQ=255)                                                    cgATGTCCGGCCCGATTACCCAGT‑GGGGCGATATGGAATTTAACGGCGCGCGTCAGACAATTAAAGACAtt                <  1:2795700/71‑1 (MQ=255)                                                    cgATGTCCGGCCCGATTACCCAGT‑GGGGCGATATGCAATTTAACGGCGCGCGTCAGGCAATTAAAGACAtt                <  1:3410666/71‑1 (MQ=255)                                                     gATGTCCGGCCCGATTACCCAGT‑GGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGACAtt                <  1:1120463/70‑1 (MQ=255)                                                     gATGTCCGGCCCGATTACCCAGT‑GGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGACAtt                <  1:4346151/70‑1 (MQ=255)                                                     gATGTCCGGCCCGATTACCCAGT‑GGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGACAtt                <  1:4282597/70‑1 (MQ=255)                                                      aTGTCCGGCCCGATTACCCAGT‑GGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGACATTaa              >  1:4080478/1‑71 (MQ=255)                                                      aTGTCCGGCCCGATTACCCAGT‑GGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGACATTaa              <  1:3645003/71‑1 (MQ=255)                                                        gTCCGGCCCGATTACCCAGT‑GGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGACATTAATg            >  1:3278404/1‑71 (MQ=255)                                                                  aTTACCCAGT‑GGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGACATTAATGCCAAAggggg  <  1:3357704/71‑1 (MQ=255)                                                                     |                                                                     CAATTTCACACACCGCTATGGCTGACGATATTAAAGTCGCCGTTGTCGGCGCGATGTCCGGCCCGATTGCCCAGT‑GGGGCGATATGGAATTTAACGGCGCGCGTCAGGCAATTAAAGACATTAATGCCAAAGGGGG  >  W3110S.gb/4042903‑4043038

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑